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Acta Parasitologica

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Volume 63, Issue 4

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First molecular detection and phylogenetic analysis of Neospora caninum DNA from naturally infected goats in Northwest Tunisia

Yosra Amdouni
  • Corresponding author
  • Laboratoire de Parasitologie, Univ. Manouba, Institution de la Recherche et de l’Enseignement Supérieur Agricoles, École Nationale de Médecine Vétérinaire de Sidi Thabet, 2020 Sidi Thabet, Tunisia
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/ Safa Amairia
  • Laboratoire de Parasitologie, Univ. Manouba, Institution de la Recherche et de l’Enseignement Supérieur Agricoles, École Nationale de Médecine Vétérinaire de Sidi Thabet, 2020 Sidi Thabet, Tunisia
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Yousra Said
  • Laboratoire de Parasitologie, Univ. Manouba, Institution de la Recherche et de l’Enseignement Supérieur Agricoles, École Nationale de Médecine Vétérinaire de Sidi Thabet, 2020 Sidi Thabet, Tunisia
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Sofia Awadi / Mohamed Gharbi
  • Laboratoire de Parasitologie, Univ. Manouba, Institution de la Recherche et de l’Enseignement Supérieur Agricoles, École Nationale de Médecine Vétérinaire de Sidi Thabet, 2020 Sidi Thabet, Tunisia
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2018-10-18 | DOI: https://doi.org/10.1515/ap-2018-0083

Abstract

Neospora caninum is an intracellular protozoan parasite from the phylum Apicomplexa, mainly associated with abortions and causing enormous economic losses. We aimed, by the present study, to estimate the molecular prevalence and phylogenetic analyses of natural infection with N. caninum in Tunisian goats. A total number of 121 meat samples were collected from slaughtered goats in the regional slaughterhouse of Béja (Northwest Tunisia) and tested from N. caninum ITS1 gene using PCR followed by sequencing of PCR products. Phylogenetic analyses were used to identify this parasite infecting goats in Nortwest Tunisia. The overall molecular prevalence was 19% (23/121). The highest molecular prevalence of N. caninum was observed in goats aged between 2 and 4 years (31.9 ± 13.27%) (P = 0.004). There was no difference in the overall molecular prevalence of N. caninum according to both localities and animal breeds. Comparison of the partial sequences of the ITS1 gene revealed 99–100% similarity with GenBank sequences. A high similarity with all the blasted genotypes was reported for N. caninum sequences. This is the first molecular study and genetic characterisation of N. caninum in North African goats.

Keywords: Neospora caninum; Tunisian goats’; meat samples; molecular identification; PCRs

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About the article

Received: 2017-10-24

Revised: 2018-05-08

Accepted: 2018-06-28

Published Online: 2018-10-18

Published in Print: 2018-12-19


Conflict of interest statement. The authors declare that they have no conflict of interests.


Citation Information: Acta Parasitologica, Volume 63, Issue 4, Pages 709–714, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2018-0083.

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