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Acta Parasitologica

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Volume 63, Issue 4


Molecular characterization and phylogenetic analysis of Taenia multiceps from China

L. Tan
  • College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province 410128, PR China
  • Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Changsha, Hunan Province 410128, PR China
  • R & D Center for Animal Reverse Vaccinology of Hunan Province, Hunan Province, PR China
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ A.B. Wang
  • College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province 410128, PR China
  • Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Changsha, Hunan Province 410128, PR China
  • R & D Center for Animal Reverse Vaccinology of Hunan Province, Hunan Province, PR China
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ S.Q. Zheng / X.L. Zhang / C.J. Huang / W. Liu
  • Corresponding author
  • College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province 410128, PR China
  • Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Changsha, Hunan Province 410128, PR China
  • R & D Center for Animal Reverse Vaccinology of Hunan Province, Hunan Province, PR China
  • College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province 510642, PR China
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2018-10-18 | DOI: https://doi.org/10.1515/ap-2018-0085


Taenia multiceps, one of the most widely distributed zoonotic tapeworm parasites, is able to parasitize the small intestine of canids. The metacestode of T.multiceps is fatal to ruminants and causes important economic losses in livestock. However, molecular characteristics of T.multiceps and coenurus in China are still unclear. In this study, 36 goat isolates of the coenurus stage and 18 dog isolates of the adult stage of T.multiceps were obtained from three geographical areas in China and the isolated parasite above were analyzed by amplifying the partial of cytochrome coxidase subunit 1(pcox1), 12S ribosomal RNA (12S rRNA) from mitochondrial DNA (mtDNA) regions and an internal transcribed spacer (ITS) of ribosomal DNA (rDNA). These DNA sequences obtained from T.multiceps and coenurus were employed to evaluate the nucleotide diversity and confirm the relationship between T.multiceps and coenurus. Sequences variation were 0–1.4%, 0–1.5%, 0–4.2% for pcox1, 12S rRNA and ITS, respectively, among T.multiceps and coenurus isolates obtained in this study. In Sichuan province, sequence variations for Coenurus cerebralis isolated from Yaan city were 0–1.4% for pcox1, 0–1.0% for 12S rRNA and 0–2.1% for ITS. In Hunan province, variations were 0–1.0%, 0–1.5% and 0–3.3% for corresponding genes for non-coenurus cerebralis isolated from Changsha city, while variations of T.multiceps isolates from Xiangxi autonomous prefecture were 0–1.0%, 0–1.1% and 0–3.4% for pcox1, 12S rRNA and ITS, respectively. Phylogenetic analysis based on pcox1 sequences indicated that all cerebral and noncerebral metacestodes belong to T.multiceps. These results provide reference values for future molecular epidemiological and biological study on T.multiceps in dogs and intermediate hosts.

Keywords: Genetic variation; mitochondrial DNA (mtDNA); internal transcribed spacer (ITS); phylogenetic analysis; Taenia multiceps; China


  • Akbari M., Moazeni M., Oryan A., Sharifiyazdi H., Amrabadi O. 2015. Experimental cerebral and non-cerebral coenurosis in goats: acomparative study on the morphological and molecular characteristics of the parasite. Veterina"ry Parasitology, 211, 201–207. CrossrefGoogle Scholar

  • Al-Riyami S., Ioannidou E., Koehler A.V., Hussain M.H., Al-Rawahi A.H., Giadinis N.D., et al. 2016. Genetic characterisation of Taenia multiceps cysts from ruminants in Greece. Infection Genetics & Evolution, 38, 110–116. CrossrefWeb of ScienceGoogle Scholar

  • Amer S., ElKhatam A., Fukuda Y., Bakr L.I., Zidan S., Elsify A., et al. 2017. Prevalence and identity of Taenia multiceps cysts "Coenurus cerebralis" in sheep in egypt. Acta Tropica, 176, 270–276. CrossrefPubMedWeb of ScienceGoogle Scholar

  • Avcioglu H., Yildirim A., Duzlu O., Inci A., Terim K.A., Balkaya I. 2011. Prevalence and molecular characterization of bovine Coenurosis from Eastern Anatolian region of Turkey. Veterinary Parasitology, 176, 59–64. DOI: /10.1016/j.vetpar.2010.10.033CrossrefWeb of SciencePubMedGoogle Scholar

  • Benifla M., Barrelly R., Shelef I., El-On J., Cohen A., Cagnano E. 2007. Huge hemispheric intraparenchymal cyst caused by Taenia multiceps in a child. Journal of Neurosurgery, 107, 511–514Web of ScienceGoogle Scholar

  • Burland T.G. 2000. DNASTAR’s Lasergene sequence analysis software. Methods in Molecular Biology, 132, 71–91Google Scholar

  • Cheng T., Gao D.Z., Zhu W.N., Fang S.F., Chen N., Zhu X.Q., et al. 2016. Genetic variability among Hymenolepis nana isolates from different geographical regions in China revealed by sequence analysis of three mitochondrial genes. Mitochondrial DNA, 27, 4646–4650. CrossrefWeb of ScienceGoogle Scholar

  • Christodoulopoulos G., Kassab A., Theodoropoulos G. 2015. Characteristics of non-cerebral coenurosis in tropical goats. Veterinary Parasitology, 211, 216–222. CrossrefPubMedWeb of ScienceGoogle Scholar

  • Christodoulopoulos G., Dinkel A., Romig T., Ebi D., Mackensted U., Loos-Frank B. 2016. Cerebral and non-cerebral coenurosis: on the genotypic and phenotypic diversity of Taenia multiceps. Veterinary Parasitology, 115, 4543–4558. CrossrefGoogle Scholar

  • Collomb J., Machouart M., Biava M.F., Brizion M., Montagne K., Plénat F., Fortier B. 2007. Contribution of NADH dehydrogenase subunit I and cytochrome C oxidase subunit I sequences toward identifying a case of human Coenuriasis in France. Journal of Parasitology, 93, 934–937Web of ScienceCrossrefGoogle Scholar

  • Dai R.S., Liu G.H., Song H.Q., Lin R.Q., Yuan Z.G., Li M.W., et al. 2012. Sequence variability -in two mitochondrial DNA regions and internal transcribed spacer among three cestodes infecting animals and humans from China. Journal of Helminthology, 86, 245–251. CrossrefPubMedWeb of ScienceGoogle Scholar

  • El-On J., Shelef I., Cagnano E., Benifla M. 2008. Taenia multiceps: a rare human cestode infection in Israel. Veterinaria Italiana, 44, 621–631PubMedGoogle Scholar

  • Gasser R.B., Zhu X., McManus D.P. 1999. NADH dehydrogenase subunit 1 and cytochrome c oxidase subunit I sequences compared for members of the genus Taenia (Cestoda). International Journal for Parasitology, 29, 1965–1970PubMedCrossrefGoogle Scholar

  • Gasser R.B. 2006. Molecular tools-advances, opportunities and prospects. Veterinary Parasitology, 136, 69–89. CrossrefPubMedGoogle Scholar

  • Jia W.Z., Yan H.B., Guo A.J., Zhu X.Q., Wang Y.C., Shi W.G., et al. 2010. Complete mitochondrial genomes of Taenia multiceps, T.hydatigena and T.pisiformis: additional molecular markers for a tapeworm genus of human and animal health significance. BMC Genomics, 11, 447. CrossrefPubMedWeb of ScienceGoogle Scholar

  • Librado P., Rozas J. 2009. DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics, 25, 1451–1452Web of ScienceCrossrefPubMedGoogle Scholar

  • Liu G.H., Lin R.Q., Li M.W., Liu W., Liu Y., Yuan Z.G, et al. 2011.The complete mitochondrial genomes of three cestode species of Taenia infecting animals and humans. Molecular Biology Reports, 38, 2249–2256. CrossrefPubMedWeb of ScienceGoogle Scholar

  • Li W.H., Jia W.Z., Qu Z.G., Xie Z.Z., Luo J.X., Yin H., et al. 2013. Molecular Characterization of Taenia multiceps Isolates from Gansu Province, China by Sequencing of Mitochondrial Cytochrome C Oxidase Subunit 1. Korean Journal of Parasitology, 51, 197–201. CrossrefWeb of ScienceGoogle Scholar

  • Nakao M., Lavikainen A., Iwaki T., Haukisalmi V., Konyaev S., Oku Y., et al. 2013. Molecular phylogeny of the genus Taenia (Cestoda: Taeniidae): proposals for the resurrection of Hydatigera Lamarck, 1816 and the creation of a new genus Versteria. International Journal of Parasitology, 2013, 427–437. CrossrefWeb of ScienceGoogle Scholar

  • Oryan A., Nazifi S., Sharifiyazdi H., Ahmadnia S. 2010. Pathological, molecular, and biochemical characterization of Coenurus gaigeri in Iranian native goats. Journal of Parasitology, 96, 961–967CrossrefWeb of ScienceGoogle Scholar

  • Oryan A., Akbari M., Moazeni M., Amrabadi O. R. 2015. Cerebral and non-cerebral Coenurosis in small ruminants. Tropical Biomedicine, 31, 1–16Google Scholar

  • Oryan A., Moazeni M., Amrabadi O., Akbari M., Sharifiyazdi H. 2015. Comparison of distribution pattern, pathogenesis and molecular characteristics of larval stages of taenia multiceps, in sheep and goats. Small Ruminant Research, 132, 44–49CrossrefWeb of ScienceGoogle Scholar

  • Rostami S., Salavati R., Beech R.N., Sharbatkhori M., Babaei Z., Saedi S., Harandi M.F. 2013. Cytochrome c oxidase subunit 1 and 12S ribosomal RNA characterization of Coenurus cerebralis from sheep in Iran. Veterinary Parasitology. 197, 141– 151. CrossrefGoogle Scholar

  • Schuster R.K., Sivakumar S., Wieckowsky T. 2010. Non-cerebral coenurosis in goats. Parasitology Research, 107, 721–726Web of SciencePubMedCrossrefGoogle Scholar

  • Sonmez B., Koroglu E., Simsek S. 2017. Molecular characterization and detection of variants of Taenia multiceps in sheep in Turkey. Parasitology, 144, 220–225. CrossrefWeb of SciencePubMedGoogle Scholar

  • Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28, 2731–2739. CrossrefWeb of SciencePubMedGoogle Scholar

  • Tappe D., Berkholz J., Mahlke U., Lobeck H., Nagel T., Haeupler A., et al. 2016. Molecular Identification of Zoonotic TirrnSe-Invasive Tapeworm Larvae Other than Taenia solium in Suspected Human. Cysticercosis Cases. Journal of Clinical Microbiology, 54, 172–174. CrossrefPubMedGoogle Scholar

  • Thompson J.D., Gibson T.J., Plewniak F.D.R., Jeanmougin F.O., Higgins D.G. 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25, 4876–4882CrossrefPubMedGoogle Scholar

  • Varcasia A., Pipia A.P., Arru D., Pes A.M., Tamponi C., Dore, et al. 2013. Morphological and molecular characterization of bovine coenurosis in sardinia, italy. Parasitology Research, 112, 2079–2082. CrossrefWeb of SciencePubMedGoogle Scholar

  • Yan H., Lou Z., Li L., Ni X., Guo A., Li H., et al. 2013. The nuclear 18S ribosomal RNA gene as a source of phylogenetic information in the genus Taenia. Parasitology Research, 112, 1343–1347. CrossrefPubMedWeb of ScienceGoogle Scholar

  • Zhang L., Hu M., Jones A., Allsopp B.A., Beveridge I., Schindler A.R., Gasser R.B. 2007. Characterization of Taenia madoquae and Taenia regis from carnivores in Kenya using genetic markers in nuclear and mitochondrial DNA, and their relationships with other selected taeniids. Molecular and Cellular Probes, 21, 379-385. CrossrefWeb of SciencePubMedGoogle Scholar

  • Zhang Y., Zhao W., Yang D., Tian Y., Zhang W., Liu A. 2018. Genetic characterization of three mitochondrial gene sequences of goat/sheep-derived coenurus cerebralis and cysticercus tenuicollis isolates in inner Mongolia, China. Parasite, 25, 1. CrossrefPubMedGoogle Scholar

  • Zhu X., Chilton N.B., Jacobs D.E., Boes J., Gasser R.B. 1999. Characterisation of Ascaris from human and pig hosts by nuclear ribosomal DNA sequences. International Journal for Parasitology, 29, 469–478PubMedGoogle Scholar

About the article

Received: 2017-12-12

Revised: 2018-05-25

Accepted: 2018-07-06

Published Online: 2018-10-18

Published in Print: 2018-12-19

Consent for publication. All animal studies/procedures of this project were approved by Hunan Agricultural University Research Ethics Committee as well as the Guide for the Care and Use of Laboratory Animals of the Ministry of Health, China.

Conflict of interest None

Citation Information: Acta Parasitologica, Volume 63, Issue 4, Pages 721–727, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2018-0085.

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