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Licensed Unlicensed Requires Authentication Published by De Gruyter October 18, 2018

Pathological and molecular identification of porcine cysticercosis in Maharashtra, India

  • Kaushik Satyaprakash EMAIL logo , Waqar A. Khan , Sandeep P. Chaudhari , Shilpshree V. Shinde , Nitin V. Kurkure and Sunil W. Kolte
From the journal Acta Parasitologica

Abstract

Porcine cysticercosis, caused by metacestodes of Taenia solium is an important emerging zoonotic disease with public health and economic significance. Pigs acquire the disease through consumption of Taenia solium eggs excreted by human tapeworm carriers. The present study was conducted to investigate the prevalence of porcine cysticercosis in Nagpur and Mumbai region of Maharashtra, India by P/M examination of carcasses followed by histopathology of affected organs in infected animals and molecular identification of cysts for confirmation. Out of 1000 pigs examined during slaughter, three pigs were found to be heavily affected with T. solium cysts giving a prevalence of 0.3%. Histological section of brain in infected animals revealed marked vascular congestion of meninges, mild neuronal degeneration, perivascular cuffing and gliosis while the liver showed the infiltration of mononuclear cell, predominantly eosinophils throughout the parenchyma. Some degree of calcification was observed in the cysts lodged in liver while calcification was not evident in case of cysts lodged in brain, tongue, diaphragm and skeletal muscle. Molecular identification by PCR using two sets of oligonucleotide primers against LSU rRNA gene and Mt-Cox1 gene of T. solium confirms the cysts to be that of T. solium. The molecular diagnostics methods have been considered for validation in conjunction with P/M inspections, parasitological and histopathological examinations. The study confirms the presence of porcine cysticercosis in the two regions and demands proper sanitary measures to minimize the risk of infection from zoonoses and food safety point of view.

  1. Interest competing.We do declare that there is no conflict of interest for the current research.

Acknowledgement

The authors would like to acknowledge Indian Council of Agricultural Research (ICAR), New Delhi for providing financial support in conducting the study.

References

Bhadrige V.V., Zende R.J., Paturkar A.M., Vaidya V.M., Deshmukh A.V. 2014. Studies on prevalence of cysticercosis and its economic impact on cattle, buffalo and pig meat production. Journal of Veterinary Public Health, 12, 22–25. 10.2016/jvph.v12i1.79Search in Google Scholar

Boa M.E., Bøgh H.O., Kassuku A.A., Nansen P. 1995. The prevalence of Taenia solium metacestodes in pigs in northern Tanzania. Journal of Helminthology, 69, 113–117. 10.1017/S0022149X00013997Search in Google Scholar

Boa M.E., Mahundi E.A., Kassuku A.A., Willingham A.L., Kyvsgaard N.C. 2006. Epidemiological survey of swine cysticercosis using ante-mortem and post-mortem examination tests in the southern highlands of Tanzania. Veterinary Parasitology, 139, 249–255. 10.1016/j.vetpar.2006.02.012Search in Google Scholar

Borkataki S., Islam S., Borkakati M.R., Goswami P., Deka D.K. 2012. Prevalence of porcine cysticercosis in Nagaon, Morigaon and Karbianglong district of Assam, India. Veterinary World, 5, 86–90. 10.5455/vetworld.2012.86-90Search in Google Scholar

Chawhan P., Singh B.B., Sharma R., Gill J.P.S. 2016. Morphological characterization of Cysticercus cellulosae in naturally infected pigs in Punjab (India). Journal of Parasitic Diseases, 40, 237– 239. 10.1007/s12639-014-0482-ySearch in Google Scholar

D’Souza P.E., Hafeez M. 1998. Incidence of Cysticercus cellulosae infection in pigs in Andhra Pradesh. Mysore Journal of Agricultural Sciences, 32, 75–78Search in Google Scholar

Garcia H.H., Del Brutto O.H. 2000. Taenia solium cysticercosis. Infectious Disease Clinics of North America, 14, 97–119. 10.1016/S0891-5520(05)70220-8Search in Google Scholar

Ito A., Wandra T., Yamasaki H., Nakao M., Sako Y., Nakaya et al. 2004. Cysticercosis/ taeniasis in Asia and the Pacific. Vectorborne Zoonotic Diseases, 4, 95–107. 10.1089/1530366041210756Search in Google Scholar

Jardim E.A.G.V., Linhares G.F.C., Torres F.A.G., Araújo J.L.B., Barbosa S.M. 2006. Specific discrimination between Taenia saginata and Taenia solium by one step PCR assay and duplex-PCR, Ciência Rural, Santa Maria, 36, 166–17210.1590/S0103-84782006000100025Search in Google Scholar

Khaing T.A., Bawm S., Wai S.S., Htut Y., Htun L.L. 2015. Epidemiological Survey on Porcine Cysticercosis in Nay Pyi Taw Area, Myanmar. Journal of Veterinary Medicine, Article ID 340828, Epub 2015 Feb 26, 10.1155/2015/340828Search in Google Scholar

Kumar S., Sinha S., Sinha S.R.P., Samantaray S., Ali I., Kumari S. 2013. Gross and histopathological changes in heart and brain in spontaneous cases of porcine cysticercosis. Journal of Veterinary Parasitology, 27, 123–125Search in Google Scholar

Le T.H., Blair D., McManus D.P. 2000. Mitochondrial genomes of human helminths and their use as markers in population genetics and phylogeny. Acta Tropica, 77, 243–256. 10.1016/S0001-706X(00)00157-1Search in Google Scholar

Mandakhalikar K.D., Jangir D., Waskar K.S. 2009. Prevalence of cysticercosis in pigs and economic losses due to condemnation of pork. Journal of Bombay Veterinary College, 17, 49– 51Search in Google Scholar

Mayta H., Gilman, R.., Prendergast, E. et al. 2008. Nested PCR for specific diagnosis of Taenia solium taeniasis. Journal of Clinical Microbiology, 46, 286–289. 10.1128/JCM.01172-07Search in Google Scholar

Mohan V.R., Jayaraman T., Babu P., Dorny P., Vercruysse J., Rajshekhar V. 2014 Prevalence and risk factors for Taenia solium taeniasis in Kaniyambadi block, Tamil Nadu, South India. Indian Journal of Public Health, 58, 202–203. 10.4103/0019-557X.138638Search in Google Scholar

Nunes C.M., Dias A.K., Dias F.E. et al. 2005. Taenia saginata: differential diagnosis of human taeniasis by polymerase chain reaction restriction fragment length polymorphism assay. Experimentl Parasitology, 110, 412–415. 10.1016/j.exppara.2005.03.008Search in Google Scholar

OIE (2018) http://www.oie.int/animal-health-in-the-world/oie-listeddiseases-2018/Search in Google Scholar

Palampalle H.Y. 2012. Studies on antigenic profile of Cysticercus cellulosae in pig to develop specific immunodiagnostic test. PhD Thesis, Maharashtra Animal and Fishery Sciences University, Nagpur, Maharashtra, IndiaSearch in Google Scholar

Prasad K.N., Chawla S., Prasad A., Tripathi M., Husain N. 2006. Clinical signs for identification of neurocysticercosis in swine naturally infected with Taenia solium. International Journal for Parasitology, 55, 51–54. 10.1016/j.parint.2006.01.002Search in Google Scholar

Prasad K.N., Prasad A., Gupta R.K., Pandey C.M., Singh U. 2007. Prevalence and associated risk factors of Taenia solium taeniasis in a rural pig farming community of north India. Transactions of the Royal Society of Tropical Medicine and Hygiene, 101, 1241–1247. 0.1016/j.trstmh.2007.04.019Search in Google Scholar

Prasad A., Gupta R.K., Pradhan S., Tripathi M., Pandey C.M., Prasad K.N. 2008. What triggers seizures in neurocysticercosis? A MRI based study in pig farming community from a district of North India. Parasitology International, 57, 166–171. 10.1016/j.parint.2007.12.001Search in Google Scholar

Rajshekhar V., Chandy M.J. 2000. Incidence of solitary cysticercus granulomas. In: Rajshekhar V, Chandy MJ, eds. Solitary cysticercus granuloma: the disappearing lesion. Chennai: Orient Longman pp 12–28Search in Google Scholar

Rajshekhar V., Joshi D.D., Doanh N.Q., De N.V., Zhou X.N. 2003. Taenia solium taeniosis/cysticercosis in Asia: epidemiology, impact and issues. Acta Tropica, 87, 53–60. 10.1016/S0001-706X(03)00055-XSearch in Google Scholar

Ramahefarisoa R.M., Rakotondrazaka M., Jambou R., Carod J.F. 2010. Comparison of ELISA and PCR assays for the diagnosis of porcine cysticercosis. Veterinary Parasitology, 173, 362–363. 10.1016/j.vetpar.2010.05.002Search in Google Scholar PubMed

Sahoo M., Singh R., Singh K.P., Sharma A.K., Saikumar G., Sylvester L. et al. 2016. Prevalence and pathology of cysticercosis in slaughtered pigs of Rohilkhand region. Indian Journal of Veterinary Pathology, 40, 69–71. 10.5958/0973-970X.2016.00014.6Search in Google Scholar

Singh A.K., Singh S.K., Prasad K.N., Singh A., Bajpai A., Rahman M. et al. 2013. Evaluation of ELISA, neck muscle, tongue and eyelid examinations for the diagnosis of swine cysticercosis in a highly endemic area of North India, Experimental Parasitology, 134, 313–317. 10.1016/j.exppara.2013.03.033Search in Google Scholar PubMed

Sreedevi C., Hafeez M., Putcha A.K., Vukka R.C., Kothapalli S.V., Krovvidi, S. 2011. PCR test for detecting Taenia solium cysticercosis in pig carcasses. Tropical Animal Health and Production, 44, 95–99. 10.1007/s11250-011-9893-2Search in Google Scholar PubMed

Sreedevi C. 2013. Diagnosis of Taenia Solium metacestode infection in pigs: A Review. International Journal of Health Sciences and Research, 1, 9–15. 10.19070/2332-2748-130003Search in Google Scholar

Torre P. de la, Merchant M. T., Willims K., Laclette J.P. 1998. Host cells in the spinal column of Taenia solium (cestoda) cysticerci. Journal of Parasitology, 84, 167–17110.2307/3284551Search in Google Scholar

Trevisan C., Mkupasi E.M., Ngowi H.A., Forkman B., Johansen M.V. 2016. Severe seizures in pigs naturally infected with Taenia solium in Tanzania. Veterinary Parasitology, 220, 67– 71. 10.1016/j.vetpar.2016.02.025Search in Google Scholar PubMed PubMed Central

Vora S.H., Motghare D.D., Ferreira A.M., Kulkarni M.S., Vaz F.S. 2008. Prevalence of human cysticercosis and taeniasis in rural Goa, India. The Journal of communicable diseases. 40, 147– 150Search in Google Scholar

Yamasaki H., Allan J.C., Sato M.O., Nakao M., Sako Y., Nakaya K. et al. 2004a. DNA differential diagnosis of taeniasis and cysticercosis by multiplex PCR. Journal of Clinical Microbiology, 42, 548–553. 10.1128/JCM.42.2.548-553.2004Search in Google Scholar PubMed PubMed Central

Yamasaki H., Matsunaga S., Yamamura K., Chang C., Kawamura S., Sako Y. et al. 2004b. Solitary Neurocysticercosis case caused by Asian genotype of Taenia solium confirmed by mitochondrial DNA analysis. Journal of Clinical Microbiology, 42, 3891–3893. 10.1128/JCM.42.8.3891-3893.2004Search in Google Scholar PubMed PubMed Central

Yera H., Dupont D., Houze S., Ben M’Rad M., Pilleux F., Sulahian A. et al. 2011. Confirmation and follow-up of neurocysticercosis by Real-Time PCR in cerebrospinal fluid samples of patients living in France. Journal of Clinical Microbiology, 49, 4338–4340. 10.1128/JCM.05839-11Search in Google Scholar PubMed PubMed Central

Received: 2018-01-16
Revised: 2018-07-25
Accepted: 2018-08-07
Published Online: 2018-10-18
Published in Print: 2018-12-19

© 2018 W. Stefański Institute of Parasitology, PAS

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