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Agricultura Tropica et Subtropica

The Journal of Czech University of Life Sciences Prague

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1801-0571
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Genetic diversity of Xanthomonas oryzae pv. oryzae in rice fields of Guilan province (Iran) using RAPD markers

Azam Taheri Shahrestani
  • Department of Plant Pathology, Faculty of Agricultural Sciences, University of Guilan, P.O. Box 41635-1314, Rasht – Iran
/ Mostafa Niknejad Kazempour
  • Department of Plant Pathology, Faculty of Agricultural Sciences, University of Guilan, P.O. Box 41635-1314, Rasht – Iran
  • :
/ Ali Akbar Ebadie
  • Rice Research Institute of Iran
/ Seyed Ali Elahinia
  • Department of Plant Pathology, Faculty of Agricultural Sciences, University of Guilan, P.O. Box 41635-1314, Rasht – Iran
Published Online: 2012-10-31 | DOI: https://doi.org/10.2478/v10295-012-0010-y

Abstract

Genetic variation in 60 isolates of Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of rice bacterial blight disease, investigated using random amplifi ed polymorphic DNA (RAPD) markers. All of 60 strains isolated and characterized through 2006-2008 in Guilan province. Three populations: a) isolates from nursery, b) isolates from fi eld, and c) isolates from panicle studied. Eight decamer primers of RAPD amplifi ed 187 polymorphic bands. The largest and smallest fragments generated by 80.7 and OPA10 primers, respectively. The minimum amount of polymorphic information content was related to OPA12 primer (0.36) and the maximum amount of that was related to OPA10 primer (0.44). Analysis of molecular variance (AMOVA) of data from RAPD analysis showed that the genetic variation within Xoo populations was greater than between populations. The similarity matrix conducted by Simple Matching coeffi cient and cluster analysis made by unweighted pair group method (UPGMA) with NTSYS-pc soft ware. At a similarity index of 0.6 the isolates were grouped into 3 clusters. These results revealed that the RAPD markers used in this study could differentiate nursery and fi eld isolates from each other.

Keywords: Xanthomonas oryzae pv. oryzae; bacterial blight of rice; RAPD markers; genetic diversity.

  • ADHIKARI T.B., MEW T.W., LEACH J. E. (1999): Genotypic and pathotypic diversity in Xanthomonas oryzae pv. oryzae in Nepal. Phytopathology 89: 687-694 [PubMed]

  • ARDALES E.Y., LEUNG H., VERACRUZ C.M., MEW T.W., LEACH J. E., NELSON R.J. (1996): Hierarchical analysis of spatial variation of the rice bacterial blight pathogene across diverse agro ecosystem in the Philippines. Phytopathology 86: 241-252

  • GANG L., CONG- FENG S., XIAO- MIA P., YUE Y., JIN-SHENG W. (2009): Analysis of pathotypic and genotypic diversity of Xanthomonas oryzae pv. oryzae in China. Journal of Phytopathology 157: 208- 218 [Web of Science]

  • GONZALEZ C., SZUREK B., MANCEAU C., MATHIEU T., SERE Y., VERDIER V. (2007): Molecular and pathotypic characterization of new Xanthomonas oryzae strains from West Africa. Molecular Plant-Microbe Interactions 20(5): 534-546 [Crossref]

  • GUPTA V. S., RAJEBHOSALE M.D., SODHI M., SUKHWINDER SIGHT, GNANAMANICKAM S. S., DHALIWAL H. S., RANJEKAR P. K. (2001): Assessment of genetic variability of Xanthomonas oryzae pv. oryzae using RAPD-PCR and IS1112-based PCR. Current Science 80(8): 1043-1049

  • HU J., ZHANG Y., QIAN W., HE C. (2007): Avirulence gene and insertion element-based RFLP as well as RAPD markers reveal high levels of genomic polymorphism in the rice pathogen Xanthomonas oryzae pv. oryzae. Systematic and Applied Microbiology 30: 587-600 [Web of Science]

  • JYUFUKU S., FURUYA N., GOTO T., TSUCHIYA K., YOSHIMURA A. (2009): Pathogenic and genetic diversity in Asian strains of Xanthomonas oryzae pv. oryzae. Faculty of Agriculture, Kyushu University 54(1): 19-23

  • KOSAWANG C., SMITAMANA P., TOOJINDA T., NILPANIT N., SIRITHUNYA P. (2006): Amplifi ed fragment length polymorphism fi ngerprinting differentiates genetic diversity of Xanthomonas oryzae pv. oryzae from northern Thailand. Phytopathology 154: 550-555

  • LEACH J. E., RHOADS M. L., VERA CRUZ C. M., WHITE F. F., MEW T. W. (1992): Assessment of genetic diversity and population structure of Xanthomonas oryzae pv. oryzae with a repetitive DNA element. Applied and Environtal Microbiology 58: 2188-2195

  • LEACH J.E., WHITE F. F., RHOADS M.L., LEUNG H. (1990): A repetitive DNA sequence differentiates Xanthomonas campesteris pv. oryzae from other pathovars of Xanthomonas campesteris. Molecular Plant-Microbe Interactions 3: 238-246

  • LEUNG H., NELSON R. J., LEACH J. E. (1993): Population structure of plant pathogenic fungi and bacteria. Advances in Plant Pathology 10: 157-205

  • MEW T. W., ALVAREZ A. M., LEACH J. E., SWINGS J. (1993): Focus on bacterial blight of rice. Plant Disease 77: 5-12

  • NEI M. (1987): Molecular phylogeny and genetic diversity analysis. Pennsylvania State University. University Park. PA 16802. USA

  • OCHIAI H., HORIONO O., MIYAJIMA K., KAKU H. (2000): Genetic diversity of Xanthomonas oryzae pv. oryzae strains from Sri Lanka. Phytopathology 90: 415-421

Mostafa Niknejad Kazempour. Department of Plant Pathology Faculty of Agricultural Sciences, University of Guilan P.O.Box. 41635-1314, Rasht - Iran Email: mniknejad34@gmail.com


Received:

Accepted:

Published Online: 2012-10-31

Published in Print: 2012-10-01


Citation Information: Agricultura tropica et subtropica. Volume 45, Issue 2, Pages 60–65, ISSN (Online) 1801-0571, DOI: https://doi.org/10.2478/v10295-012-0010-y, October 2012

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