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Agricultura Tropica et Subtropica

The Journal of Mendel University in Brno, Czech Republic

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1801-0571
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Preliminary Study of Diversity of Plukenetia volubilis Based on the Morphological and Genetic Characteristics

Blanka Krivankova
  • Corresponding author
  • Department of Crop Sciences and Agroforestry, Institute of Tropics and Subtropics, Czech University of Life Sciences Prague, Czech Republic
  • Email:
/ Petra Hlasna Cepkova
  • Department of Crop Sciences and Agroforestry, Institute of Tropics and Subtropics, Czech University of Life Sciences Prague, Czech Republic
/ Martin Ocelak
  • Department of Crop Sciences and Agroforestry, Institute of Tropics and Subtropics, Czech University of Life Sciences Prague, Czech Republic
/ Gaelle Juton
  • Agrocampus Ouest Centre d’Angers, Angers, France
/ Miroslav Bechyne
  • Department of Crop Sciences and Agroforestry, Institute of Tropics and Subtropics, Czech University of Life Sciences Prague, Czech Republic
/ Bohdan Lojka
  • Department of Crop Sciences and Agroforestry, Institute of Tropics and Subtropics, Czech University of Life Sciences Prague, Czech Republic
Published Online: 2012-11-01 | DOI: https://doi.org/10.2478/v10295-012-0023-6

Abstract

The aim of this study was to make an initial step in clarification of morphological diversity of sacha inchi (Plukenetia volubilis L.), a traditional oilseed crop, together with use of ISSR primers for a preliminary assessment of genetic variability in the species. Quantitave and qualitative morphological data together with leaf samples for twenty plant individuals of sacha inchi from five distinct populations were gathered in three different communities neighbouring the city of Pucallpa in Peru. Morphological data were processed into PCA analysis graph while for DNA analysis the method of fingerprinting processed into PCO graph and dendrogram was used. Visible morphological dissimilarities in the plant appearance were confirmed and observable differences in phenotypical characteristics among different types of associated cultivations were recorded. For genetic variability twelve from eighteen ISSR primers used and tested in the study could be used directly for further molecular pursuit on this species. Dendrogram with six main clusters was created and thus confirmed polymorphism as allogamy plants shows. Obtained results contributed to the identification of suitable method for further assessment of genetic variability within this species. New horizons for the focus of further research and improvements in onward development and genetic conservation of sacha inchi cultivation were identified, thus linking the observation of morphological features with DNA analysis proved as effective.

Keywords: diversity; ISSR markers; morphological characters; Peruvian Amazon; Plukenetia volubilis; sacha inchi.

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About the article

Received:

Accepted:

Published Online: 2012-11-01

Published in Print: 2012-10-01


Citation Information: , ISSN (Online) 1801-0571, DOI: https://doi.org/10.2478/v10295-012-0023-6.

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