Jump to ContentJump to Main Navigation
Show Summary Details

Agricultura Tropica et Subtropica

The Journal of Czech University of Life Sciences Prague

4 Issues per year

Open Access
Online
ISSN
1801-0571
See all formats and pricing




In vitro Conservation of Smallanthus sonchifolius under Slow-Growth Conditions

Iva Skalova
  • Institute of Tropics and Subtropics, Czech University of Life Sciences Prague, Czech Republic
  • :
/ Iva Viehmannova
  • Institute of Tropics and Subtropics, Czech University of Life Sciences Prague, Czech Republic
/ Jan Vitamvas
  • Faculty of Forestry and Wood Sciences, Czech University of Life Sciences Prague, Czech Republic
Published Online: 2012-11-01 | DOI: https://doi.org/10.2478/v10295-012-0024-5

Abstract

The aim of this work was to optimize in vitro storage of yacon (Smallanthus sonchifolius) under slow-growth conditions. Mediumterm conservation was tested on ½ MS supplemented with mannitol and sorbitol (1-3%), BAP (5 mg l-1), or MS and ½ MS medium free of plant growth regulators. Within 60 days, height of plants, number of nodes, number of roots and their length were measured in all of these treatments. The results revealed that there was a decline in plant growth along with the increase in the concentrations of mannitol (1.88, 1.49 and 1.07 cm for 10, 20 and 30 g l-1 mannitol) and sorbitol (3.66, 3.19 and 1.74 cm for 10, 20 and 30 g l-1 sorbitol), and along with the decrease of nutrient concentration in the cultivation medium (4.74 and 2.24 cm for MS and ½ MS medium). The survival of explants was found to be minimal at the highest concentrations of osmotic regulators (70% in mannitol and 50% in sorbitol) and on the medium supplemented with BAP (60%), while maximal on MS medium, ½ MS medium and on ½ MS medium with addition of 10 g l-1 mannitol (in all cases 100%). On full-strength MS medium and medium with addition of BAP plant growth was rapid, suggesting that these media could be rather used for multiplication of yacon. On the basis of our results it can be concluded, that for medium-term conservation of yacon can be satisfactory used ½ MS medium and media supplemented by 10 or 20 g l-1 mannitol or sorbitol.

Keywords: Germplasm; medium-term preservation; yacon; cultivation medium; 6-benzylaminopurine; mannitol; sorbitol.

  • Charrier A., Dereuddre J., Engelmann F. (1991): The implications of biotechnology in germplasm conservation and utilization. Crop Genetic Resources of Africa. In: Ng N.Q., Perrino P., Attere F., Zedan H., editors. Proceedings of an International Conference on Crop Genetic Resources of Africa. Ibadan, Nigeria, IITA/IBPGR/CNR, pp. 279-286.

  • Chicata N. (1998): Variability of botanical seeds and comparison of progenies and clones of germplasm of yacon (Polymnia sonchifolia). Master thesis, Universidad nacional de San Antonio Abad del Cusco, Peru. (in Spanish)

  • da Silva T.L., Scherwinski-Pereira J.E. (2011): In vitro conservation of Piper aduncum and Piper hispidinervum under slow-growth conditions. Pesquisa Agropecuária Brasileira, 46(4): 383-389.

  • Engelmann F. (1998): In vitro conservation of horticultural genetic resources. Review of the state of the art. World Conference on Horticultural Research, Rome, Italy, June 17-20.

  • Engelmann F. (1991): In vitro conservation of tropical plant germplasm - a review. Euphytica, 57(3): 227-243. [Crossref]

  • Fernández C.E., Kučera L. (1997): Determination of number of chromosomes in select ecotypes of yacon (Polymnia sonchifolia Poeppig and Endlicher) cultivated in vitro. Agricultura Tropica et Subtropica, 30: 89-93.

  • Grau A., Rea J. (1997): Yacon. Smallanthus sonchifolius (Poep.&Endl.) H. Robinson. In: Hermann M., Heller J., editors. Andean roots and tubers: Ahipa, arracacha, maca and yacon. Rome: IPGRI, pp. 199-242.

  • Lata H., Chandra S., Mehmedic Z., Khan I.A., ElSohly M.A. (2012): In vitro germplasm conservation of high Δ9-tetrahydrocannabinol yielding elite clones of Cannabis sativa L. under slow growth conditions. Acta Physiologiae Plantarum 34(2): 743-750.

  • Malaurie B., Trouslot M.F., Bethaud J., Bousalem M., Pinel A., Dubern J. (1998): Medium-term and long-term in vitro conservation and safe international exchange of yam (Dioscorea spp.) germplasm. Electronic Journal of Biotechnology 1(3): 26-27.

  • Murashige T., Skoog F. (1962): A revised medium for rapid growth and bioassays with tabacco tissue cultures. Physiologia Plantarum 15: 473-497. [Crossref]

  • Renau-Morata B., Arrillaga I., Segura J. (2006): In vitro storage of cedar shoot cultures under minimal growth conditions. Plant Cell Reports 25(7): 636-642. [Crossref]

  • Sarkar D., Naik P.S. (1998): Factors affecting minimal growth conservation of potato microplants in vitro. Euphytica 102(2): 275-280. [Crossref]

  • Tehrim S., Sajid G.M. (2011): In vitro Establishment, Conservation and its Implications for Grape Germplasm Biodiversity. Romanian Biotechnological Letters 16(6): 6781-6789.

  • Tyagi R.K., Goswani R., Sanayaima R., Singh R., Tandon R., Agrawal A. (2009): Micropropagation and slow growth conservation of cardamom (Elettaria cardamomum Maton). In Vitro Cellular & Developmental Biology - Plant 45(6): 721-729.

  • Viehmannová I. (2009): In vitro polyploidization and protoplast cultures in yacon [Smallanthus sonchifolius (Poeppig & Endlicher) H. Robinson]. Ph.D. Thesis, Czech University of Life Sciences Prague, Institute of Tropics and Subtropics, 111 p. (in Czech).

  • Withers L.A. (1991): Crop strategies for roots and tubers: Potato - a model for refinement, Yam - a problem for development. In: Becker B., editor. Workshop on Conservation of Plant Genetic Resources. Bonn. ATSAF/IBPGR, pp. 11-14.


Received:

Accepted:

Published Online: 2012-11-01

Published in Print: 2012-10-01


Citation Information: . Volume 45, Issue 3, Pages 147–150, ISSN (Online) 1801-0571, DOI: https://doi.org/10.2478/v10295-012-0024-5, November 2012

This content is open access.

Comments (0)

Please log in or register to comment.