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Agricultura Tropica et Subtropica

The Journal of Mendel University in Brno, Czech Republic

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Saliva Crystallization in Cattle: New Possibility for Early Pregnancy Diagnosis?

Iva Skalova
  • Department of Animal Science and Food Processing, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Czech Republic
/ Tamara Fedorova
  • Corresponding author
  • Department of Animal Science and Food Processing, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Czech Republic
  • Email:
/ Karolina Brandlova
  • Department of Animal Science and Food Processing, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Czech Republic
Published Online: 2013-11-16 | DOI: https://doi.org/10.2478/ats-2013-0018


Saliva sampling is a non-invasive, simple and low-cost procedure. The aim of this study was to confirm the presence and changes of saliva crystallization in domestic cattle during synchronized oestrous cycle and early pregnancy. We verified saliva crystallization as a method for early pregnancy diagnosis. Eight Holstein cows were included into the research. The samples were collected daily from 16th day before to 34th day after artificial insemination (in total 51 days). We observed the following types of crystallization: none, dotted, branch-like, fir-like, fern-like and combinations of them and an atypical pattern. We confirmed the presence of saliva crystallization in cattle and its changes during oestrus synchronization process, insemination and post-insemination periods. We found significant differences in pregnant and non-pregnant animals between 20th and 29th day after insemination. We concluded that pregnancy diagnosis by saliva crystallization might be possible but the practical application of this method is currently unfeasible.

Keywords : crystalline patterns; arborisation; ferning; non-invasive pregnancy diagnosis; reproductive cycle

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About the article

Published Online: 2013-11-16

Published in Print: 2013-09-01

Citation Information: Agricultura tropica et subtropica, ISSN (Online) 1801-0571, DOI: https://doi.org/10.2478/ats-2013-0018.

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