Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Acta Universitatis Cibiniensis. Series E: Food Technology

The Journal of „Lucian Blaga“ University of Sibiu

2 Issues per year

Open Access
Online
ISSN
2344-150X
See all formats and pricing
More options …

Enzyme-Assisted Extraction of Polyphenols From Rose (Rosa Damascena Mill.) Petals

Krasimira Kalcheva-Karadzhova / Vasil Shikov / Kiril Mihalev / Georgi Dobrev / Danka Ludneva / Nikolai Penov
Published Online: 2014-12-30 | DOI: https://doi.org/10.2478/aucft-2014-0015

Abstract

: The efficiency of enzyme-assisted extraction for the recovery of polyphenols from rose (Rosa damascena Mill.) petals was evaluated performing a simplex centroid experimental design for mixture with three components (pectinolytic, cellulolytic and hemicellulolytic preparation). The ternary enzyme combinations leaded to the highest contents of total polyphenols, reaching 43% higher average value as compared to the control (without enzymatic treatment) sample. Enzymatic treatments also enhanced (9−25%) the extractability of total anthocyanins. The results obtained demonstrate that enzyme-assisted extraction improves the recovery of polyphenolic antioxidants from rose petals, especially using ternary enzyme combinations, comprising pectinolytic, cellulolytic and hemicellulolytic preparation.

Keywords : polyphenols; enzyme-assisted extraction; Rosa damascena

References

  • 1. Anesini C. & Perez C. (1993). Screening of plants used in Argentine folk medicine for antimicrobial activity. J. Ethnopharmacol., 39, 119−128. DOI: 10.1016/0378-8741(93)90027-3.CrossrefGoogle Scholar

  • 2. Benzie I.F.F. & Strain J.J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Analytical Biochemistry, 239(1), 70−76. DOI: 10.1006/abio.1996.0292.CrossrefGoogle Scholar

  • 3. Brand-Williams W., Cuvelier M.E. & Berset C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT - Food Science and Technology, 28(1), 25−30. DOI: 10.1016/S0023-6438(95)80008-5.CrossrefGoogle Scholar

  • 4. Cho E.J., Yokozawa T., Rhyu D.Y., Kim S.C., Shibahara N. & Park J.C. (2003). Study on the inhibitory effects of Korean medicinal plants and their main compounds on the 1,1-diphenyl-2-picrylhydrazyl radical, Phytomedicine, 10(6-7), 544−551. DOI: 10.1078/094471103322331520.Google Scholar

  • 5. Choi E.M. & Hwang J.K. (2003). Investigations of anti-inflammatory and antinociceptiveactivities of Piper cubeba, Physalis angulata and Rosa hybrida. J. Ethnopharmacol., 89(1), 171−175. DOI: 10.1016/S0378-8741(03)00280-0.CrossrefGoogle Scholar

  • 6. Dinkova R., Shikov, V., Mihalev K., Velchev Z., Dinkov H. & Mollov P. (2012). Changes in the total anthocyanins and polyphenols during processing of wild berries into freshly pressed juices. Journal of EcoAgriTourism, 8(1), 254−259.Google Scholar

  • 7. Dixit N., Tripathi C. & Upadhyay R. (1976). The antifungal substance of rose flowers (Rosa indica). Econ. Bot., 30, 371−374. DOI:10.1007/BF02904658.CrossrefGoogle Scholar

  • 8. Dobrev G. & Zhekova B. (2012). Biosynthesis, purification and characterization of endodlucanase from xylanase, produsing strain Aspergilus niger B03. Brazilian Journal of Microbiology, 43(1), 70−77. DOI: 10.1590/S1517-83822012000100008.CrossrefGoogle Scholar

  • 9. Dobrev G., Pishtiyski I., Stanchev V. & Mircheva R. (2007), Optimization of nutrient medium containing agricultural wastes for xylanase production by Aspergillus niger B03 using optimal composite design. Bioresource Technology, 98(14), 2671−2678. DOI:10.1016/j.biortech.2006.09.022.Web of ScienceCrossrefGoogle Scholar

  • 10. Fenglin H., Ruili L., Bao H. & Liang M. (2004). Free radical scavenging activity of extracts prepared from fresh leaves of selected Chinese medicinal plants. Fitoterapia, 75(1), 14−23. DOI: 10.1016/j.fitote.2003.07.003.CrossrefGoogle Scholar

  • 11. Ginova A., Mihalev K. & Kondakova V. (2013). Antioxidant capacity of petals and leaves from different rose (Rosa damascena Mill.) plantations in Bulgaria. International Journal of Pure Applied Bioscience, 1, 38−43.Google Scholar

  • 12. Giusti M.M. & Wrolstad R.E. (2001). Characterization and measurement of anthocyanins by UV-visible spectroscopy. In R.E. Wrolstad (Ed.), Current Protocols in Food Analytical Chemistry (pp. F1.2.1−F1.2.13), New York: Wiley.Google Scholar

  • 13. Kaverzneva E.D. (1971). Assay for determination of proteolytic activity of complex enzymes and proteinases. Prikladnaja biochimia i Mikrobiologia, 7, 225−228 (in Rusian). Google Scholar

  • 14. Magalhães L.M., Segundo M.A., Reis S. & Lima J.L.F.C. (2008). Methodological aspects about in vitro evaluation of antioxidant properties. Analytica Chimica Acta, 613(1), 1−19. DOI: 10.1016/j.aca.2008.02.047CrossrefWeb of ScienceGoogle Scholar

  • 15. Mahmood N., Piacente S., Pizza C., Burke A., Khan A.I. & Hay A.J. (1996). The anti-HIVactivity and mechanisms of action of pure compounds isolated from Rosa damascena. Biochem. Biophys. Res. Comm., 229(1), 73−79. DOI: 10.1006/bbrc.1996.1759.CrossrefGoogle Scholar

  • 16. Miller G.L. (1959). Use of dinitrosalicyclic acid reagent for determination of reducing sugar. J. Anal. Chem., 31(3), 426−428. DOI: 10.1021/ac60147a030.CrossrefGoogle Scholar

  • 17. Miron T.L., Herrero M. & Ibáñez E. (2013). Enrichment of antioxidant compounds from lemon balm (Melissa officinalis) by pressurized liquid extraction and enzyme-assisted extraction. Journal of Chromatography A, 1288(3), 1−9. DOI: 10.1016/j.chroma.2013.02.075.CrossrefWeb of ScienceGoogle Scholar

  • 18. Ng T.B., He J.S., Niu S.M., Zhao L., Pi Z.F., Shao W.& Liu F. (2004). A gallic acid derivative and polysaccharides with antioxidative activity from rose (Rosa rugosa) flowers. J. Pharm. Pharmacol., 56, 537−545. DOI: 10.1211/0022357022944.CrossrefGoogle Scholar

  • 19. Perez C. & Anesini C. (1994). In vitro antibacterial activity of Argentine folk medicinal plants against Salmonella typhi. J. Ethnopharmacol., 44(1), 41−46. DOI: 10.1016/0378-8741(94)90097-3.CrossrefGoogle Scholar

  • 20. Pinelo-Jiménez M. & Meyer A.S. (2008). Enzyme-assisted extraction of antioxidants: Release of phenols from vegetal matrixes. Electronic Journal of Environmental, Agricultural and Food Chemistry, 7(8), 3217−3220.Google Scholar

  • 21. Ponpium P., Ratanakhanokchai K. & Kyu K. (2000). Isolation and properties of a cellulosome-type multienzyme complex of the thermophilic Bacteroides sp. strain P-1. Enzyme Microbial. Technol., 26(5-6), 459−465. DOI: 10.1016/S0141-0229(99)00195-7.Google Scholar

  • 22. Singleton V.L. & Rossi J.A. Jr. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16, 144−158.Google Scholar

  • 23. VanderJagt T.J., Ghattas R., VanderJagt D.J., Crossey M. & Glew R.H. (2002). Comparison of the total antioxidant content of 30 widely used medicinal plants of New Mexico. Life Sci., 70(9), 1035−1040 DOI: 10.1016/S0024-3205(01)01481-3.CrossrefGoogle Scholar

  • 24. Vinokur Y., Rodov V., Reznick N., Goldman G., Horev B., Umiel N. & Friedman H. (2006). Rose petal tea as an antioxidant-rich beverage: cultivar effects, J. Food Sci., 71, S42−S47. DOI: 10.1111/j.1365-2621.2006.tb12404.x. CrossrefGoogle Scholar

About the article

Published Online: 2014-12-30

Published in Print: 2014-12-01


Citation Information: Acta Universitatis Cibiniensis. Series E: Food Technology, Volume 18, Issue 2, Pages 65–72, ISSN (Online) 2344-150X, DOI: https://doi.org/10.2478/aucft-2014-0015.

Export Citation

© Lucian Blaga University. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Anton Slavov, Ivelina Vasileva, Lyubomir Stefanov, and Albena Stoyanova
Reviews in Environmental Science and Bio/Technology, 2017, Volume 16, Number 2, Page 309

Comments (0)

Please log in or register to comment.
Log in