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Acta Universitatis Cibiniensis. Series E: Food Technology

The Journal of „Lucian Blaga“ University of Sibiu

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2344-150X
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Identification of Probiotic Strains from Human Milk in Breastfed Infants with Respiratory Infections

Bogdan Neamtu
  • Corresponding author
  • “Lucian Blaga” University of Sibiu, Faculty of Medicine “Victor Papilian”, str. Lucian Blaga , nr. 2A, Sibiu 550169, Romania
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/ Ovidiu Tita
  • “Lucian Blaga” University of Sibiu, Faculty of Agricultural Sciences, Food Industry and Environment Protection, Romania
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/ Mihai Neamtu
  • “Lucian Blaga” University of Sibiu, Faculty of Medicine “Victor Papilian”, str. Lucian Blaga , nr. 2A, Sibiu 550169, Romania
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/ Mihaela Tita
  • “Lucian Blaga” University of Sibiu, Faculty of Agricultural Sciences, Food Industry and Environment Protection, Romania
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/ Mirela Hila
  • Pediatric Clinical Hospital from Sibiu, Pompeiu Onofrei Street, no: 2-4, 550166 Sibiu, Romania
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/ Ionela Maniu
  • “Lucian Blaga” University of Sibiu, Faculty of Sciences, Department of Mathematics and Informatics, Romania
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Published Online: 2014-12-30 | DOI: https://doi.org/10.2478/aucft-2014-0016

Abstract

Isolation and industrial exploitation of probiotics from human milk is a goal for worldwide milk biotechnology centres because of their modulation effect on the immune system in infants and adults. In the proposed study we have analysed fermentation patterns of Lactobacilli isolated from human milk, the reliability of API 50 CH carbohydrate fermentation system and a possible link between lactose concentrations and fermentation profiles on carbohydrates. We had succesfully identified three species of Lactobacillus (paracasei ssp paracasei, fermentum, acidophilus) and one unsatisfactory identification of Lactoccocus lactis ssp lactis. These strains had different carbohydrate fermentation patterns but with common characteristics and showed no statistically significant correlations between their carbohydrate metabolic trends and lactose concentrations in the milk samples.

Keywords: human milk; lactobacilli; API 50CH identification; lactose concentrat

References

  • 1. Agrawal R. (2005) Probiotic: an emerging food supplement with health benefits. Food Biotechnology, 19(3), 227-246; DOI: 10.1080/08905430500316474CrossrefGoogle Scholar

  • 2. Ashraf M., Arshad M., Siddique M. and Muhammad G. (2009) In Vitro Screening of Locally Isolated Lactobacillus Species for Probiotic Properties; Pakistan Veterinary Journal., 29(4), 186-190;eISSN: 2074-7764Google Scholar

  • 3. Boyd M. A. , Antonio May A.D. and Hillier L. Sharon (2005), Comparison of API 50 CH Strips to Whole-Chromosomal DNA Probes for Identification of Lactobacillus Species; Journal of Clinical Microbiology , 43(10), 5309-5311. DOI :10.1128/JCM.43.10.5309-5311.2005CrossrefGoogle Scholar

  • 4. Burgain J., Gaiani C., Linder M., Scher J. (2011) Encapsulation of probiotic living cells : From laboratory scale to industrial applications, Journal of Food Engineering 104(4), 467-483 ; DOI : 10. 1016/j.foodeng.2010.12.031;CrossrefWeb of ScienceGoogle Scholar

  • 5. Çakmakci S., Bülent Ç., Turgut T., Gurses M., Erdogan A. (2012) Probiotic Properties, Sensory Qualities and Storage Stability of Probiotic Banana Yogurts; Turkish Journal Veterinary Animal Science 36(3), 231-237, DOI:10.3906/vet-1007-2CrossrefGoogle Scholar

  • 6. Chirico G., Marzollo R., Cortinovis S., Fonte C., Gasparoni A., (2008) Antiinfective properties of Human Milk, The Journal of Nutrition: 138(9),1801S-1806S, eISSN :1541-6100;Google Scholar

  • 7. Dimitonova P. S., Bakalov Vladimirov B., Aleksandrova-Georgieva R. N., Danova S. Trifonova (2008), Phenotypic and molecular identification of lactobacilli isolated from vaginal secretions, Journal of Microbiology, Immunology and Infection, 41(6), 469-477, eISSN: 1995-9133 ;Google Scholar

  • 8. Doutoum A. A., Tidjani A. , Abdelaziz Arada I., Tidjani S. M. T, Balde M., Sylla K. S. B. , Seydi Mg and Toguebaye B.S.(2013), Identification of the lactic microorganism responsible for milk cogulation in Abeche (Chad), African Journal of Food Science, 7 (5), 103-106, DOI : 10.5897/AJFS12.161;CrossrefGoogle Scholar

  • 9. Herbel R. Stefan, Vahjen Wilfried, Wieler Lothar H and Guenther Sebastian; (2013); Timely Approaches to Identify Probiotic Species of the Genus Lactobacillus. Gut Pathogens; 5(1); DOI:10.1186/1757-4749-5-27Web of ScienceCrossrefGoogle Scholar

  • 10. Hyun-jue Kim, Han-seung Shin,Woel-kyu Ha, Hee-jin Yang and Soowon Lee (2006), Characterization of Lactic Bacterial Strains Isolated from Raw Milk; Asian-Australasian Journal of Animal Sciences,19(1); 131-136, DOI:10.5713/ajas2011.11177CrossrefGoogle Scholar

  • 11. Kadere T.T. , Kutima M.P., (2012), Isolation and Identification of Lactic Acid Bacteria in Coconut Toddy (MNAZI), Journal of Asian Scientific Research , 2(12):807-819, eISSN:2223-1331Google Scholar

  • 12. Karna B.K.L., Emata O.C. and Barraquio V.L. (2007), Lactic Acid and Probiotic Bacteria from Fermented and Probiotic Dairy Products ; Science Diliman 19(2), 23-24, eISSN :2310-9173;Google Scholar

  • 13. Khalil R , El-Halafawy K, Mahrous H, Kamaly K, Frank J, Soda El M (April 2007) Evaluation of the Probiotic Potential of Lactic Acid Bacteria Isolated from Faeces of Breast Fed Infants in Egypt; African Journal of Biotechnology , 6 (7), 939-949, DOI: 10.5897/AJB2007.000-2114;CrossrefGoogle Scholar

  • 14. Kizerwetter-Swida M. and Binek M. (2005) Selection of Potentially Probiotic Lactobacillus Strains Towards their Inhibitory Activity against Poultry Enteropathogenic Bacteria Polish Journal of Microbiology, 54 (4), 287-294; eISSN 1733-1331Google Scholar

  • 15. Klaenhammer T. R., Azcarate-Peril M.A., Altermann E., and Barrangou R. (2007) Influence of the Dairy Environment on Gene Expression and Substrate Utilization in Lactic Acid Bacteria, The Journal of Nutrition, 137(3): 748S-750S, eISSN :1541-6100Google Scholar

  • 16. Kos B., Šušković J., Beganović J., Gjuračić K., Frece J., Iannaccone C., Canganella F. (2008). Characterization of the Three Selected Probiotic Strains for the Application in Food Industry, World Journal of Microbiology and Biotechnology 24(5):699-707, DOI 10.1007/s11274-007-9528-y;CrossrefGoogle Scholar

  • 17. Leuschner R. , Kneife W., Vernoux J.-P. , Stanton C. and Aldamiz P. (2002). Methods for the Official Control of Probiotics used as Feed Additives, volume 2, Luxembourg: European Comission Community Research,;Google Scholar

  • 18. Malek Abd R., Sallehhuddin Bin Hamdan, Hesham A El Enshasy, Nor Zalina Othman, Noor Azwani Zainol, Mohamad R Sarmidi, A Aziz (2010); Production of Lactobacillus salivarius, a new probiotic strain isolated from human breast milk, in semi-industrial scale and studies on its functional characterization; In A. Mendez-Villas(Ed), In Current Research, Technology and Education Topics, Applied Microbiology and Microbial Biotechnology (pp.1196-1204 ) Spain: Formatex Research CenterGoogle Scholar

  • 19. Marroki A., Zúñiga M., Kihal M., Pérez-Martínez G. (2011), Characterization of Lactobacillus from Algerian goat’s milk base don phenotypic, 16S rDNA sequencing and their technological properties, Brazilian Journal of Microbiology, 42(1), 158-171, DOI:101590/S1517-838222011000100020Web of ScienceGoogle Scholar

  • 20. O’Donnell M. M, O’Toole P. W and Ross R. P. (2013), Catabolic flexibility of mammalian-associated lactobacilli, Microbial Cell Factories,12(48), DOI:10.1186/1475-2859-12-48;Web of ScienceCrossrefGoogle Scholar

  • 21. Yavuzdurmaz H., Sebnem H. (2011) Selection of potential probiotic lactobacillus strains from human milk; In Food Product Engineering &Functional Foods: 11th International Congress on Engineering and Food, (pp2043-2044), Athens, Greece: CosmowareGoogle Scholar

  • 22. Zavisic G, Petricevic S, Radulovic Z, Begovic J, Golic N, Topisirovic L, Strahinic I. (2012) ;Probiotic Features of Two Oral Lactobacillus Isolates, Brazilian Journal of Microbiology, 43(1), 418-428; DOI:10.1590/S1517-83822012000100050CrossrefGoogle Scholar

  • 23. ZhengY., LuY., Wang J., Yang L., Pan C., Huang Y. (2013), Probiotic Properties of Lactobacillus Strains Isolated from Tibetan Kefir Grains; PLOS ONE 8(7); DOI : 10.371/journal.pone.0069868 Google Scholar

About the article

Published Online: 2014-12-30

Published in Print: 2014-12-01


Citation Information: Acta Universitatis Cibiniensis. Series E: Food Technology, Volume 18, Issue 2, Pages 73–84, ISSN (Online) 2344-150X, DOI: https://doi.org/10.2478/aucft-2014-0016.

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