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Acta Universitatis Cibiniensis. Series E: Food Technology

The Journal of „Lucian Blaga“ University of Sibiu

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2344-150X
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Effect Of Five Proteases Including Alcalase, Flavourzyme, Papain, Proteinase K And Trypsin On Antioxidative Activities Of Casein Hydrolysate From Goat Milk

Guowei Shu
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  • School of Food and Biological Engineering, Shaanxi University of Science & Technology, Xi’ an, 710021, China
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/ Qian Zhang
  • School of Food and Biological Engineering, Shaanxi University of Science & Technology, Xi’ an, 710021, China
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/ He Chen
  • School of Food and Biological Engineering, Shaanxi University of Science & Technology, Xi’ an, 710021, China
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/ Hongchang Wan / Hong Li
Published Online: 2015-12-22 | DOI: https://doi.org/10.1515/aucft-2015-0015

Abstract

Oxidation was related to the pathogenesis of human diseases. Adequate intake of antioxidant activity of food can reduce the levels of free radicals, prevent lipid peroxidation, and help the body against diseases. In the paper, casein from goat milk was hydrolyzed by five commercial proteases, namely, Alcalase, flavourzyme, papain, proteinase K and trypsin. The antioxidant activities of casein hydrolysates were assessed by evaluating hydrolysis degree, DPPH radical-scavenging activity, metal-chelating activity and superoxide radical scavenging activity. The results showed as follows: the DH of proteinase K, Alcalase, and trypsin were higher significantly than those of papain and flavourzyme. The Fe2+-chelation activity and superoxide radical scavenging activity of casein hydrolysates from goat milk by Alcalase was higher than the others, the DPPH scavenging activities of casein hydrolysates by Alcalase and papain was higher than the others and the DPPH scavenging activities by Alcalase and papain had no significant diffierence (p<0.05), so the optimal proteinase for hydrolysis casein from goat milk to produce antioxidant peptide was Alcalase.

Keywords: goat milk casein; Alcalase; enzymatic hydrolysis; antioxidative peptides

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About the article

Published Online: 2015-12-22

Published in Print: 2015-12-01


Citation Information: Acta Universitatis Cibiniensis. Series E: Food Technology, Volume 19, Issue 2, Pages 65–74, ISSN (Online) 2344-150X, DOI: https://doi.org/10.1515/aucft-2015-0015.

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© 2015 Guowei Shu et al., published by De Gruyter Open. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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