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Ovidius University Annals of Chemistry

Analele Universitatii "Ovidius" Constanta - Seria Chimie

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Fat soluble vitamins and fatty acid composition of wild Black sea mussel, rapana and shrimp

Albena Merdzhanova / Diana A. Dobreva / Mona Stancheva / Lubomir Makedonski
Published Online: 2014-09-12 | DOI: https://doi.org/10.2478/auoc-2014-0003


Many studies suggest that marine molluscs are one of the most important dietary sources of fat soluble vitamins (E, D3 and A) and essential fatty acids (FA). The most commercially important species from the Bulgarian Black Sea are the Black mussel, rapana and shrimp. There is scarce information in the scientific literature about fat soluble vitamins and FA composition of these Black Sea molluscs. The aims of the present study are to determine and compare fat soluble vitamins content as well as relative daily intake, FA composition and atherogenic index (IA), thrombogenicity index (IT) and flesh-lipid quality index (FLQ) in wild Black Sea mussel (Mytilus galloprovincialis), rapana (Rapana venosa) and shrimp (Crangon crangon). Fat soluble vitamins were analysed simultaneously using RP-HPLC system. The FA profile was analysed by GC-MS. All of the analysed samples presented significant amounts of vitamin E, followed by vitamin A and D3. Black Sea molluscs are excellent sources of fat soluble vitamins, especially for vitamin D3 - one survey provides more than 100% of the RDI established in Bulgaria. The FA composition of total lipids showed significant differences and the present study revealed that SFA content was significantly higher than MUFA (p<0.001) and PUFA (p<0.001) (SFA>PUFA>MUFA) in shrimp and mussel whereas rapana showed opposite trends (PUFA>SFA>MUFA). The omega6/omega3 and PUFA/SFA ratios of the analysed species were greater than the FAO/WHO recommendations.

Keywords: Mytilus sp.; Rapana sp.; Crangon sp.; fat soluble vitamins; fatty acids; Bulgarian Black Sea coast


  • [1] E. Petrova and St. Stoykov, Agricultural Science and Technology 3 (4), 368 - 373 (2011).Google Scholar

  • [2] E. Petrova and St. Stoykov, Macedonian Journal of Animal Science 1 (1), 223-226 (2011).Google Scholar

  • [3] S. Moncheva, J. Namiesnik, R Apak, P. Arancibia-Avila, F. Toledo, S. Kang, S. Jung and S. Gorinstein, Chemistry and Ecology 27, 1), 31-41 (2011).CrossrefGoogle Scholar

  • [4] J. Campos, and H.W Van der Veer, Oceanogr. Mar. Biol. Annu. Rev. 46, 65-104 (2008)Google Scholar

  • [5] Ribarova F. Food and vitamins Sofia, Bulgaria. (2007).Google Scholar

  • [6] K. W. Lee, A. D.Blann, Y.Gregory and H. Lip, Thrombosis Research 118, 305-312 (2006).CrossrefGoogle Scholar

  • [7] A. K. Dutta-Roy, Platelets 13, 67-75 (2002).Google Scholar

  • [8] P. Kris - Etherton, W. Harris., L. Appel, Arterioscler Thromb Vasc Biol 23, 20-30 (2003), http://www.atvbaha.org. Google Scholar

  • [9] *** FAO, Food and Nutrition, Fats and fatty acids in human nutrition Report of an expert consultation, Paper 91, FAO, (Final report). (2010).Google Scholar

  • [10] D.A. Dobreva, B.Galunska and M.Stancheva, Scripta Scientifica Medica, Varna Medical University 43, 276-279 (2011).Google Scholar

  • [11] E. Bligh, and W. Dyer, Canadian Journal of Biochemistry and Physiology 37, 913-917 (1959).Google Scholar

  • [12] *** BDS EN ISO 5509 (2000)Google Scholar

  • [13] *** BDS EN ISO 5508 (2000)Google Scholar

  • [14] H. Hosseini, M. Mahmoudzadeh, M. Rezaei, L. Mahmoudzadeh, R. Khaksar, N. Khosroshahi and A. Babakhani, Food chemistry 148, 86-91 (2014)Google Scholar

  • [15] A. Simopoulos and L. Cleland, World Rev Nutr Diet. Basel, Karger 92, 1-22 (2003).CrossrefGoogle Scholar

  • [16] T. Ulbricht and D.Southgate, Lancet 338, 985-992 (1991).Google Scholar

  • [17] L. Senso, M.D.Suarez, T.Ruiz-Cara and M.Garcıa-Gallego, Food Chemistry 101, 298-307 (2007).Google Scholar

  • [18] G. Abrami, F. Natiello, P. Bronzi, D. McKenzie, L. Bolis and E. Aggrade, Comparative Biochemistry and Physiology 1/2, 71-82 (1992).Google Scholar

  • [19] R. Sirbu, A.Bechir, T.Negreanu-Pîrjol, C.Sava, B. Negreanu-Pîrjol, T. Zaharia, C. Ursache and R. M. Stoicescu, Scientific Study & Research Chemistry & Chemical Engineering, Biotechnology, Food Industry 12 (3), 221 - 228 (2011).Google Scholar

  • [20] E. Prato, A.Danieli, M. Maffia and F. Biandolino, Zoological Studies 49, (2), 211-219 (2010).Google Scholar

  • [21] V. Ventrella, M. Pirini, A. Palgliarani, F. Trombetti, M.P. Manuzzi and A.R. Borgatti, Comparative Biochemistry and Physiology, Part B 149, 241-250 (2008).Google Scholar

  • [22] H. Turan, Y, Kaya and E. Erdem, Journal of Aquatic Food Product Technology 20, 100-107 (2011).CrossrefGoogle Scholar

  • [23] G. MacDonald, Identification of Health and Nutritional Benefits of New Zealand Aquaculture Seafood’s, Report, MacDonald and Associates Ltd, Nelson (2010). Google Scholar

  • [24] *** Whole Food Catalog (2011), http://wholefoodcatalog.info/food/mussel(raw)/ Google Scholar

  • [25] *** Danish Food Composition Databank, Department of Nutrition, National Food Institute (2009).Google Scholar

  • [26] V. Öhrvik, A. Malmborg, I. Mattisson, S. Wretling and C. Åstrand, Fish, shellfish and fish products - analysis of nutrients, LIVSMEDELS VERKET, NATIONAL FOOD AGENCY, Sweden (2012).Google Scholar

  • [27] *** FNB (2011): Dietary reference intakes for Calcium and Vitamin D, http://books.nap.edu/openbook.php?record_id=13050. Google Scholar

  • [28] *** EFSA, Tolerable upper intake levels for vitamins and minerals, Scientific Committee on Food Scientific Panel on Dietetic Products, Nutrition and Allergies,(2006) http://www.efsa.europa.eu/en/ndatopics/docs/ndatolerableuil.pdf Google Scholar

  • [29] *** Ordinance 23 / 19.07.2005 on the physiological feeding of population http://bg.wikipedia.org/wiki/#cite_note-echo-9 Google Scholar

  • [30] D. Ezgeta-Balic, M. Najdek, M. Peharda and M. Blažina, Aquaculture 334-337, 89-100 (2012).Google Scholar

  • [31] E. Orban, G. Di Lena, T. Nevigato, I. Casini, A. Marzetti and R. Caproni, Food Chemistry 77, 57-65 (2002).CrossrefGoogle Scholar

  • [32] S. Zlatanos, EJEAFChe, 7(6), 3015-3019 (2008).Google Scholar

  • [33] D. Badiu, A. M.Balu, L. Barbes, R. Luque, R. Nita, M. Radu, E. Tanase and N. Rosoiu, Lipids 43, 892-841 (2008).Google Scholar

  • [34] A. Mika, M. Golebiowski, E.Skorkovski and P. Stepnowski, International Journal of Oceanography and Hydrobiology 41 (2), 1-7 (2012).Google Scholar

  • [35] P. Vernocchia, M. MaVei, R. Lanciotti, G. Suzzi and F. Gardini, Food control 18, 1575-1583 (2007).Google Scholar

  • [36] Y.P. Zaitsev, Fisheries Oceanography 1 (2), 180-189 (1992).Google Scholar

  • [37] S. Saglik and S. Imre, Turkish J Marine Sciences, 3 (3), 179-189 (1997).Google Scholar

  • [38] P. Sriket, S. Benjakul, W. Visessanguan and K. Kijroongrojana, Food Chemistry 103, 1199-1207 (2007).Google Scholar

  • [39] *** Department of Health, Nutritional aspects of cardiovascular disease: Health and social subsects. HMSO, London (1994)Google Scholar

  • [40] L. Morbidini, Sarti D.M., Pollidori P. and Valigi A. Opinions Mediterraneenes. Serie A: Seminares Mediterraneens 46, 29-33 (2001).Google Scholar

  • [41] H.Ouraji, B.Shabanpur, A.Abediankenari, A. Shabani, A. Nezami, M. Sudagar and S. Faghani, Journal of the Science of Food and Agriculture, 89, 993-997 (2009). Google Scholar

About the article

Received: 2014-04-04

Accepted: 2014-05-07

Published Online: 2014-09-12

Published in Print: 2014-06-01

Citation Information: Analele Universitatii "Ovidius" Constanta - Seria Chimie, Volume 25, Issue 1, Pages 15–23, ISSN (Online) 1223-7221, DOI: https://doi.org/10.2478/auoc-2014-0003.

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