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Bio-Algorithms and Med-Systems

Editor-in-Chief: Roterman-Konieczna , Irena

CiteScore 2018: 0.29

SCImago Journal Rank (SJR) 2018: 0.129
Source Normalized Impact per Paper (SNIP) 2018: 0.324

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Cell proliferation induced by modified cationic dextran

Kamil Kamiński / Krystyna Stalińska
  • Department of Cell Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland
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/ Anna Niziołek / Maria Wróbel
  • Medical Biochemistry, Jagiellonian University Medical College, Kopernika 7C, 31-034 Krakow, Poland
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/ Maria Nowakowska / Marta Kaczor-Kamińska
  • Corresponding author
  • Medical Biochemistry, Jagiellonian University Medical College, Kopernika 7C, 31-034 Krakow, Poland
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Published Online: 2018-12-07 | DOI: https://doi.org/10.1515/bams-2018-0036


The interaction between oppositely charged membranes and polycations causes cell aggregation, loss of membrane fluidity, and membrane degeneration and may cause an increase of its permeability. Unfortunately, the interaction is the reason why the use of polycations in medicine is severely limited. Therefore, in this paper, we share our observations related to the action of 40-kDa dextran modified using glycidyltrimethylammonium chloride, resulting in increased fibroblast cell proliferation. Using viability and proliferation tests [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, crystal violet, 3H-thymidine incorporation], we have observed that cationic dextran derivatives exert a positive impact on nonepithelial cell proliferation in vitro. This phenomenon has been noted for human and mouse fibroblasts and several other nonepithelial cell lines. However, the effect seems to be most pronounced for fibroblast cell lines. The presented studies allow to examine the impact of the polymer structure and the methods of its cationic modification on this newly observed phenomenon. The observation is unique because positively charged macromolecules usually exhibit high toxicity in all cell types in vitro.

This article offers supplementary material which is provided at the end of the article.

Keywords: dextran derivative; nonepithelial cells; polycations; proproliferation effect


  • [1]

    Fischer D, Li Y, Ahlemeyer B, Krieglstein J, Kissel T. In vitro cytotoxicity testing of polycations: influence of polymer structure on cell viability and hemolysis. Biomaterials 2003;24:1121–31.PubMedCrossrefGoogle Scholar

  • [2]

    Elferink JG. Changes of plasma membrane permeability in neutrophils treated with polycations. Inflammation 1991;15:103–15.PubMedCrossrefGoogle Scholar

  • [3]

    Monnery BD, Wright M, Cavill R, Hoogenboom R, Shaunak S, Steinke JH, et al. Cytotoxicity of polycations: relationship of molecular weight and the hydrolytic theory of the mechanism of toxicity. Int J Pharm 2017;521:249–58.CrossrefWeb of SciencePubMedGoogle Scholar

  • [4]

    Kalaska B, Kaminski K, Sokolowska E, Czaplicki D, Kujdowicz M, Stalinska K, et al. Nonclinical evaluation of novel cationically modified polysaccharide antidotes for unfractionated heparin. PLoS One 2015;10:e0119486.Web of SciencePubMedGoogle Scholar

  • [5]

    Yudovin-Farber I, Gurt I, Hope R, Domb AJ, Katz E. Inhibition of herpes simplex virus by polyamines. Antivir Chem Chemother 2009;20:87–98.CrossrefPubMedGoogle Scholar

  • [6]

    Marini JJ, Wheeler AP. Critical care medicine. Philadelphia: Wolters Kluwer/Lippincott Williams & Wilkins, 2010.Google Scholar

  • [7]

    Lewis SL. Medical surgical nursing. St. Louis, MI, USA: Elsevier - Mosby, 2017.Google Scholar

  • [8]

    Neu B, Wenby R, Meiselman HJ. Effects of dextran molecular weight on red blood cell aggregation. Biophys J 2008;95:3059–65.Web of ScienceCrossrefPubMedGoogle Scholar

  • [9]

    Rosenblum WI. Effects of dextran-40 on blood viscosity in experimental macroglobulinaemia. Nature 1968;218:591–3.CrossrefPubMedGoogle Scholar

  • [10]

    Kalaska B, Sokolowska E, Kaminski K, Szczubialka K, Kramkowski K, Mogielnicki A, et al. Cationic derivative of dextran reverses anticoagulant activity of unfractionated heparin in animal models of arterial and venous thrombosis. Eur J Pharmacol 2012;686:81–9.Web of ScienceCrossrefPubMedGoogle Scholar

  • [11]

    Sznarkowska A, Olszewski R, Zawacka-Pankau J. Pharmacological activation of tumor suppressor, wild-type p53 as a promising strategy to fight cancer. Postępy Hig Med Dosw 2010;64:396–407.Google Scholar

  • [12]

    Tassi E, Al-Attar A, Aigner A, Swift MR, McDonnell K, Karavanov A, et al. Enhancement of fibroblast growth factor (FGF) activity by an FGF-binding protein. J Biol Chem 2001;276:40247–53.CrossrefPubMedGoogle Scholar

  • [13]

    Chu H, Johanson NR, Mason NS, Wang Y. A [polycation:heparin] complex releases growth factors with enhanced bioactivity. J Control Release 2011;150:157–63.PubMedCrossrefWeb of ScienceGoogle Scholar

About the article

Received: 2018-10-23

Accepted: 2018-11-12

Published Online: 2018-12-07

Conflict of interests: The authors declare no conflict of interest.

Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

Research funding: The Polish National Science Centre (grant no. UMO-2013/09/D/ST5/03864).

Employment or leadership: None declared.

Honorarium: None declared.

Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

Citation Information: Bio-Algorithms and Med-Systems, Volume 14, Issue 4, 20180036, ISSN (Online) 1896-530X, DOI: https://doi.org/10.1515/bams-2018-0036.

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