Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred


IMPACT FACTOR 2018: 3.014
5-year IMPACT FACTOR: 3.162

CiteScore 2018: 3.09

SCImago Journal Rank (SJR) 2018: 1.482
Source Normalized Impact per Paper (SNIP) 2018: 0.820

Online
ISSN
1437-4315
See all formats and pricing
More options …
Volume 380, Issue 10

Issues

Defining the Location and Function of Domains of McrB by Deletion Mutagenesis

Uwe Pieper / Thomas Schweitzer / Detlef H. Groll / Alfred Pingoud
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/BC.1999.155

Abstract

The GTP-dependent restriction endonuclease McrBC of E. coli K12, which recognizes cytosine-methylated DNA, consists of two protein subunits, McrB and McrC. We have investigated the structural assignment and interdependence of the McrB subunit functions, namely (i) specific DNA recognition and (ii) GTP binding and hydrolysis. Extending earlier work, we have produced McrB variants comprising N- and C-terminal fragments. The variants McrB1–162 and McrB1–170 are still capable of specific DNA binding. McrB169–465 shows GTP binding and hydrolysis characteristics indistinguishable from full-length McrB as well as wild-type like interaction with McrC. Thus, DNA and GTP binding are spatially separated on the McrB molecule, and the respective domains function quite independently.

About the article

Published Online: 2005-06-01

Published in Print: 1999-10-12


Citation Information: Biological Chemistry, Volume 380, Issue 10, Pages 1225–1230, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.1999.155.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Neha Nirwan, Pratima Singh, Gyana Gourab Mishra, Christopher M Johnson, Mark D Szczelkun, Katsuaki Inoue, Kutti R Vinothkumar, and Kayarat Saikrishnan
Nucleic Acids Research, 2018
[2]
Christopher J. Hosford and Joshua S. Chappie
Journal of Biological Chemistry, 2018, Volume 293, Number 30, Page 11758
[3]
Rasa Sukackaite, Arunas Lagunavicius, Kornelijus Stankevicius, Claus Urbanke, Česlovas Venclovas, and Virginijus Siksnys
Nucleic Acids Research, 2007, Volume 35, Number 7, Page 2377
[5]
Philippe Leprohon, Hélène Gingras, Siham Ouennane, Sylvain Moineau, and Marc Ouellette
BMC Genomics, 2015, Volume 16, Number 1
[6]
E. V. Zemlyanskaya and S. Kh. Degtyarev
Molecular Biology, 2013, Volume 47, Number 6, Page 784
[7]
Vivek Anantharaman, Lakshminarayan M. Iyer, and L. Aravind
Molecular BioSystems, 2012, Volume 8, Number 12, Page 3142
[8]
Rasa Sukackaite, Saulius Grazulis, Gintautas Tamulaitis, and Virginijus Siksnys
Nucleic Acids Research, 2012, Volume 40, Number 15, Page 7552

Comments (0)

Please log in or register to comment.
Log in