Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred


IMPACT FACTOR 2018: 3.014
5-year IMPACT FACTOR: 3.162

CiteScore 2018: 3.09

SCImago Journal Rank (SJR) 2018: 1.482
Source Normalized Impact per Paper (SNIP) 2018: 0.820

Online
ISSN
1437-4315
See all formats and pricing
More options …
Volume 380, Issue 3

Issues

Yeast Cells Allow High-Level Expression and Formation of Polyomavirus-Like Particles

K. Sasnauskas / O. Buzaite / F. Vogel / B. Jandrig / R. Razanskas / J. Staniulis / S. Scherneck / D. H. Krüger / R. Ulrich
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/BC.1999.050

Abstract

Polyomavirus-derived virus-like particles (VLPs) have been described as potential carriers for encapsidation of nucleic acids in gene therapy. Although VLPs can be generated in E. coli or insect cells, the yeast expression system should be advantageous as it is well established for the biotechnological generation of products for human use, especially because they are free of toxins hazardous for humans. We selected the yeast Saccharomyces cerevisiae for expression of the major capsid protein VP1 of a non-human polyomavirus, the hamster polyomavirus (HaPV). Two entire HaPV VP1- coding sequences, starting with the authentic and a second upstream ATG, respectively, were subcloned and expressed to high levels in Saccharomyces cerevisiae. The expressed VP1 assembled spontaneously into VLPs with a structure resembling that of the native HaPV capsid. Determination of the subcellular localization revealed a nuclear localization of some particles formed by the N-terminally extended VP1, whereas particles formed by the authentic VP1 were found mainly in the cytoplasmic compartment.

About the article

Published Online: 2005-06-01

Published in Print: 1999-03-01


Citation Information: Biological Chemistry, Volume 380, Issue 3, Pages 381–386, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.1999.050.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Aliona Špakova, Eugenijus Šimoliūnas, Raminta Batiuškaitė, Simonas Pajeda, Rolandas Meškys, and Rasa Petraitytė-Burneikienė
Viruses, 2019, Volume 11, Number 3, Page 208
[2]
Dace Skrastina, Ivars Petrovskis, Rasa Petraityte, Irina Sominskaya, Velta Ose, Ilva Liekniņa, Janis Bogans, Kestutis Sasnauskas, and Paul Pumpens
Clinical and Vaccine Immunology, 2013, Volume 20, Number 11, Page 1719
[3]
Martynas Simanavicius, Paulius Lukas Tamosiunas, Rasa Petraityte-Burneikiene, Reimar Johne, Rainer G. Ulrich, Aurelija Zvirbliene, and Indre Kucinskaite-Kodze
Applied Microbiology and Biotechnology, 2017
[4]
Claudia Simon, Thomas Klose, Sabine Herbst, Bong Gyoon Han, Andrea Sinz, Robert M. Glaeser, Milton T. Stubbs, and Hauke Lilie
Journal of Biological Chemistry, 2014, Volume 289, Number 15, Page 10411
[5]
Ugo Moens, Andi Krumbholz, Bernhard Ehlers, Roland Zell, Reimar Johne, Sébastien Calvignac-Spencer, and Chris Lauber
Infection, Genetics and Evolution, 2017, Volume 54, Page 18
[6]
Aistė Bulavaitė, Rita Lasickienė, Paulius Lukas Tamošiūnas, Martynas Simanavičius, Kęstutis Sasnauskas, and Aurelija Žvirblienė
Applied Microbiology and Biotechnology, 2017, Volume 101, Number 7, Page 2991
[7]
Juozas Nainys, Albertas Timinskas, Julia Schneider, Rainer G. Ulrich, Alma Gedvilaite, and Cristina Costa
PLOS ONE, 2015, Volume 10, Number 10, Page e0140916
[8]
Monika Valaviciute, Milda Norkiene, Karolis Goda, Rimantas Slibinskas, and Alma Gedvilaite
Archives of Virology, 2016, Volume 161, Number 7, Page 1807
[9]
Alma Gedvilaite, Indre Kucinskaite-Kodze, Rita Lasickiene, Albertas Timinskas, Ausra Vaitiekaite, Danguole Ziogiene, and Aurelija Zvirbliene
Viruses, 2015, Volume 7, Number 8, Page 4204
[10]
Paulius Lukas Tamošiūnas, Rasa Petraitytė-Burneikienė, Aistė Bulavaitė, Kornelija Marcinkevičiūtė, Karolis Simutis, Rita Lasickienė, Regina Firantienė, Regina Ėmužytė, Aurelija Žvirblienė, and Kęstutis Sasnauskas
Applied Microbiology and Biotechnology, 2016, Volume 100, Number 11, Page 4935
[11]
Aistė Bulavaitė, Rita Lasickienė, Aušra Vaitiekaitė, Kęstutis Sasnauskas, and Aurelija Žvirblienė
Applied Microbiology and Biotechnology, 2016, Volume 100, Number 10, Page 4523
[12]
Milda Pleckaityte, Corinna M. Bremer, Alma Gedvilaite, Indre Kucinskaite-Kodze, Dieter Glebe, and Aurelija Zvirbliene
BMC Biotechnology, 2015, Volume 15, Number 1
[13]
Milda Norkiene, Jomante Stonyte, Danguole Ziogiene, Egle Mazeike, Kestutis Sasnauskas, and Alma Gedvilaite
BMC Biotechnology, 2015, Volume 15, Number 1
[14]
Emeline V. B. Catrice and Frank Sainsbury
Molecular Biotechnology, 2015, Volume 57, Number 10, Page 904
[15]
Juozas Nainys, Rita Lasickiene, Rasa Petraityte-Burneikiene, Jonas Dabrisius, Raimundas Lelesius, Vilimas Sereika, Aurelija Zvirbliene, Kestutis Sasnauskas, and Alma Gedvilaite
BMC Biotechnology, 2014, Volume 14, Number 1
[16]
I. Todorova, I. Iliev, A. Gedvilaitė, A. Zvirbliene, K. Sasnauskas, and E. Shikova
Biotechnology & Biotechnological Equipment, 2009, Volume 23, Number sup1, Page 806
[17]
Aurelija Zvirbliene, Indre Kucinskaite-Kodze, Ausra Razanskiene, Rasa Petraityte-Burneikiene, Boris Klempa, Rainer Ulrich, and Alma Gedvilaite
Viruses, 2014, Volume 6, Number 2, Page 640
[18]
Alma Gedvilaite, Rasa Jomantiene, Jonas Dabrisius, Milda Norkiene, and Robert E. Davis
Microbiological Research, 2014, Volume 169, Number 5-6, Page 388
[19]
Erik A. Teunissen, Markus de Raad, and Enrico Mastrobattista
Journal of Controlled Release, 2013, Volume 172, Number 1, Page 305
[20]
Helena Faust, Kristin Andersson, Johanna Ekström, Maria Hortlund, Trude Eid Robsahm, and Joakim Dillner
International Journal of Cancer, 2014, Volume 134, Number 4, Page 844
[21]
Ugo Moens, Marijke Van Ghelue, Xiaobo Song, and Bernhard Ehlers
Reviews in Medical Virology, 2013, Volume 23, Number 4, Page 250
[22]
Claudia Simon, Sebastian Schaepe, Karin Breunig, and Hauke Lilie
Preparative Biochemistry and Biotechnology, 2013, Volume 43, Number 2, Page 217
[23]
A. Zielonka, A. Gedvilaite, J. Reetz, U. Rosler, H. Muller, and R. Johne
Journal of General Virology, 2012, Volume 93, Number Pt_12, Page 2658
[24]
Alma Gedvilaite, Cornelius Frömmel, Kestutis Sasnauskas, Burkhard Micheel, Muhsin Özel, Olaf Behrsing, Juozas Staniulis, Burkhard Jandrig, Siegfried Scherneck, and Rainer Ulrich
Virology, 2000, Volume 273, Number 1, Page 21
[25]
Alma Gedvilaite, David C. Dorn, Kestutis Sasnauskas, Gabriele Pecher, Aiste Bulavaite, Robert Lawatscheck, Juozas Staniulis, Tina Dalianis, Torbjörn Ramqvist, Günther Schönrich, Martin J. Raftery, and Rainer Ulrich
Virology, 2006, Volume 354, Number 2, Page 252
[26]
Z Palková, T Adamec, D Liebl, J Štokrová, and J Forstová
FEBS Letters, 2000, Volume 478, Number 3, Page 281
[27]
Janis Freivalds, Andris Dislers, Velta Ose, Paul Pumpens, Kaspars Tars, and Andris Kazaks
Protein Expression and Purification, 2011, Volume 75, Number 2, Page 218
[28]
Milda Pleckaityte, Aurelija Zvirbliene, Indre Sezaite, and Alma Gedvilaite
Microbial Cell Factories, 2011, Volume 10, Number 1, Page 109
[30]
Antony D. Hale, Danguol≐ Bartkevičiūte, Aušra Dargevičiūt≐, Li Jin, Wendy Knowles, Juozas Staniulis, David W.G. Brown, and Kestutis Sasnauskas
Journal of Virological Methods, 2002, Volume 104, Number 1, Page 93
[32]
A. Zvirbliene, L. Samonskyte, A. Gedvilaite, T. Voronkova, R. Ulrich, and K. Sasnauskas
Journal of Immunological Methods, 2006, Volume 311, Number 1-2, Page 57
[33]
Rasa Petraitytė-Burneikienė, Kristina Nalivaiko, Rita Lasickienė, Regina Firantienė, Regina Ėmužytė, Kęstutis Sasnauskas, and Aurelija Žvirblienė
Virus Research, 2011, Volume 161, Number 2, Page 131
[34]
Rasa Petraityte, Paulius L. Tamosiunas, Mindaugas Juozapaitis, Aurelija Zvirbliene, Kestutis Sasnauskas, Brian Shiell, Gail Russell, John Bingham, and Wojtek P. Michalski
Virus Research, 2009, Volume 145, Number 1, Page 92
[35]
Mindaugas Juozapaitis, Andrius Serva, Aurelija Zvirbliene, Rimantas Slibinskas, Juozas Staniulis, Kestutis Sasnauskas, Brian J. Shiell, Lin-Fa Wang, and Wojtek P. Michalski
Virus Research, 2007, Volume 124, Number 1-2, Page 95
[36]
Anja Zielonka, Ernst J. Verschoor, Alma Gedvilaite, Uwe Roesler, Hermann Müller, and Reimar Johne
Virus Research, 2011, Volume 155, Number 2, Page 514
[37]
Mindaugas Juozapaitis, Aurelija Zvirbliene, Indre Kucinskaite, Indre Sezaite, Rimantas Slibinskas, Mayte Coiras, Fernando de Ory Manchon, María Rosa López-Huertas, Pilar Pérez-Breña, Juozas Staniulis, Irena Narkeviciute, and Kestutis Sasnauskas
Virus Research, 2008, Volume 133, Number 2, Page 178
[38]
Rimantas Slibinskas, Dhanraj Samuel, Alma Gedvilaite, Juozas Staniulis, and Kestutis Sasnauskas
Journal of Biotechnology, 2004, Volume 107, Number 2, Page 115
[39]
Rimantas Slibinskas, Aurelija Zvirbliene, Alma Gedvilaite, Dhanraj Samuel, Li Jin, Stuart Beard, Juozas Staniulis, and Kestutis Sasnauskas
Journal of Biotechnology, 2003, Volume 103, Number 1, Page 43
[40]
L. Antoniukas, H. Grammel, and U. Reichl
Journal of Biotechnology, 2006, Volume 124, Number 2, Page 347
[41]
Janis Freivalds, Andris Dislers, Velta Ose, Dace Skrastina, Indulis Cielens, Paul Pumpens, Kestutis Sasnauskas, and Andris Kazaks
Journal of Biotechnology, 2006, Volume 123, Number 3, Page 297
[42]
Mindaugas Juozapaitis, Andrius Serva, Indre Kucinskaite, Aurelija Zvirbliene, Rimantas Slibinskas, Juozas Staniulis, Kestutis Sasnauskas, Brian J. Shiell, Timothy R. Bowden, and Wojtek P. Michalski
Journal of Biotechnology, 2007, Volume 130, Number 4, Page 441
[43]
Aušra Razanskiene, Jonas Schmidt, Astrid Geldmacher, Andreas Ritzi, Matthias Niedrig, Åke Lundkvist, Detlev H. Krüger, Helga Meisel, Kestutis Sasnauskas, and Rainer Ulrich
Journal of Biotechnology, 2004, Volume 111, Number 3, Page 319
[44]
Wilson Caparrós-Wanderley, Barnaby Clark, and Beverly E. Griffin
Vaccine, 2004, Volume 22, Number 3-4, Page 352
[45]
Ausra Dargeviciute, Katarina Brus Sjölander, Kestutis Sasnauskas, Detlev H Krüger, Helga Meisel, Rainer Ulrich, and Åke Lundkvist
Vaccine, 2002, Volume 20, Number 29-30, Page 3523
[46]
Helena Faust, Diana V. Pastrana, Christopher B. Buck, Joakim Dillner, and Johanna Ekström
The Journal of Infectious Diseases, 2011, Volume 203, Number 8, Page 1096
[47]
David C. Dorn, Robert Lawatscheck, Aurelija Zvirbliene, Egle Aleksaite, Gabriele Pecher, Kestutis Sasnauskas, Muhsin Özel, Martin Raftery, Günther Schönrich, Rainer G. Ulrich, and Alma Gedvilaite
Viral Immunology, 2008, Volume 21, Number 1, Page 12
[48]
Robert Lawatscheck, Egle Aleksaite, Jörg A. Schenk, Burkhard Micheel, Burkhard Jandrig, Gudrun Holland, Kestutis Sasnauskas, Alma Gedvilaite, and Rainer G. Ulrich
Viral Immunology, 2007, Volume 20, Number 3, Page 453
[49]
Tatyana Voronkova, Adrian Grosch, Andris Kazaks, Velta Ose, Dace Skrastina, Kestutis Sasnauskas, Burkhard Jandrig, Wolfgang Arnold, Siegfried Scherneck, Paul Pumpens, and Rainer Ulrich
Viral Immunology, 2002, Volume 15, Number 4, Page 627
[50]
HASSEN SIRAY, CORNELIUS FRÖMMEL, TATYANA VORONKOVA, STEFANIE HAHN, WOLFGANG ARNOLD, JENS SCHNEIDER-MERGENER, SIEGFRIED SCHERNECK, and RAINER ULRICH
Viral Immunology, 2000, Volume 13, Number 4, Page 533
[51]
Alma Gedvilaite, Aurelija Zvirbliene, Juozas Staniulis, Kestutis Sasnauskas, Detlev H. Krüger, and Rainer Ulrich
Viral Immunology, 2004, Volume 17, Number 1, Page 51
[52]
Y. Sidorenko, L. Antoniukas, J. Schulze-Horsel, A. Kremling, and U. Reichl
Engineering in Life Sciences, 2008, Volume 8, Number 4, Page 399
[53]
Linas Antoniukas, Hartmut Grammel, Kestutis Sasnauskas, and Udo Reichl
Biotechnology Letters, 2008, Volume 30, Number 3, Page 415
[54]
Indre Kucinskaite, Mindaugas Juozapaitis, Andrius Serva, Aurelija Zvirbliene, Nicholas Johnson, Juozas Staniulis, Anthony R. Fooks, Thomas Müller, Kestutis Sasnauskas, and Rainer G. Ulrich
Virus Genes, 2007, Volume 35, Number 3, Page 521
[55]
Annika Lundstig, Pär Stattin, Kenneth Persson, Kestutis Sasnauskas, Raphael P. Viscidi, Randi Elin Gislefoss, and Joakim Dillner
International Journal of Cancer, 2007, Volume 121, Number 5, Page 1098
[56]
Tatyana Voronkova, Andris Kazaks, Velta Ose, Muhsin Özel, Siegfried Scherneck, Paul Pumpens, and Rainer Ulrich
Virus Genes, 2007, Volume 34, Number 3, Page 303
[57]
A. Gedvilaite, E. Aleksaite, J. Staniulis, R. Ulrich, and K. Sasnauskas
Archives of Virology, 2006, Volume 151, Number 9, Page 1811
[58]
Annika Stolt, Mimmi Kjellin, Kestutis Sasnauskas, Tapio Luostarinen, Pentti Koskela, Matti Lehtinen, and Joakim Dillner
International Journal of Cancer, 2005, Volume 113, Number 3, Page 393
[59]
Dhanraj Samuel, Kestutis Sasnauskas, Li Jin, Stuart Beard, Aurelija Zvirbliene, Alma Gedvilaite, and Bernard Cohen
Journal of Medical Virology, 2002, Volume 66, Number 1, Page 123

Comments (0)

Please log in or register to comment.
Log in