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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred

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Volume 381, Issue 11 (Nov 2000)


Identification of the 50S Ribosomal Proteins from the Eubacterium Thermus thermophilus

K.R. Katsani / P. Tsiboli / K. Anagnostopoulos / H. Urlaub / T. Choli-Papadopoulou
Published Online: 2005-07-05 | DOI: https://doi.org/10.1515/BC.2000.133


The total protein mixture from the 50S subunit (TP-50) of the eubacterium Thermus thermophilus was characterized after blotting onto PVDF membranes from two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and sequencing. The proteins were numbered according to their primary structure similarity with their counterparts from other species. One of them has been marked with an asterisk, namely L*23, because unlike the other known ribosomal proteins it shows a very low degree of homology.

A highly acidic 5S rRNA binding protein, TL5, was characterized and compared with the available primary structure information.

Proteins L1 and L4 migrate similarly on 2D-PAGE. Protein L4, essential for protein biosynthesis, is N-terminally blocked and shows a strikingly low homology to other L4 proteins. In addition to L4, two other proteins, namely L10 and L11, were found to be N-terminally blocked.

In conclusion, 33 proteins from the large subunit were identified, including TL5. Homologs to rpL25 and rpL26 were not found.

About the article

Published Online: 2005-07-05

Published in Print: 2000-11-15

Citation Information: Biological Chemistry, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2000.133.

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Biological Chemistry, 2004, Volume 385, Number 1

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