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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Turk, Boris / Wittinghofer, Alfred

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Characterization of a Receptor for Heat Shock Protein 70 on Macrophages and Monocytes

Holger Sondermann / Thalia Becker / Mark Mayhew / Felix Wieland / Franz-Ulrich Hartl
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/BC.2000.144


Heat shock proteins (Hsps) and molecular chaperones isolated from tumors or virally infected cells elicit an efficient CD8+ T cell response against bound antigenic peptides. This immune response is mediated by presentation of the peptides on MHC class I complexes of antigen-presenting cells (APCs), but the cellular mechanism of this presentation process is not yet understood. Here we provide evidence for the existence of a proteinaceous receptor on the surface of APCs that is specific for mammalian Hsp70. Using a flow cytometry-based assay, saturable binding of Hsp70 to the cell surface of macrophages and peripheral blood monocytes, but not of lymphocytes, can be demonstrated. The affinity of the receptor is in the sub-micromolar range (Kd < 100nm). Only mammalian Hsc70/Hsp70, but not bacterial Hsp70, is bound with high affinity. Subsequent to binding, Hsp70 is taken up by endocytosis, resulting in an intracellular localization. Our results suggest that receptor-mediated endocytosis forms the basis for the demonstrated efficacy of Hsp70-peptide complexes as anti-tumor vaccines.

Published Online: 2005-06-01

Published in Print: 2000-12-18

Citation Information: Biological Chemistry. Volume 381, Issue 12, Pages 1165–1174, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2000.144, June 2005

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