Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred

IMPACT FACTOR 2018: 3.014
5-year IMPACT FACTOR: 3.162

CiteScore 2018: 3.09

SCImago Journal Rank (SJR) 2018: 1.482
Source Normalized Impact per Paper (SNIP) 2018: 0.820

See all formats and pricing
More options …
Volume 381, Issue 3


Interaction of E. coli Single-Stranded DNA Binding Protein (SSB) with Exonuclease I. The Carboxy-Terminus of SSB Is the Recognition Site for the Nuclease

Jochen Genschel / Ute Curth / Claus Urbanke
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/BC.2000.025


The 3[']-5['] singlestranded DNA (ssDNA) degrading exonuclease I of E. coli directly interacts with the E. coli ssDNA binding protein (EcoSSB). Analytical ultracentrifugation shows that all 4 carboxytermini of an EcoSSB tetramer bind exonuclease I. Binding is weakened by increasing salt concentrations, indicating the involvement of the negatively charged amino acids of the carboxyterminus of SSB. Mutant SSB proteins EcoSSBP176S (ssb-113) and EcoSSBF177C do not bind to exonuclease I while EcoSSBG15D (ssb-3) does bind. In a coprecipitation assay we show that the absence of the last ten amino acids (PMDFDDDIPF) completely abolishes binding of EcoSSB to exonuclease I. The interaction does not depend on the presence of the correct aminoterminal DNA binding domain or the amino acid sequences between the DNA binding domain and the last ten amino acids. A synthetic peptide (WMDFDDDIPF), corresponding to the last nine amino acids of EcoSSB, specifically inhibits the interaction. Both EcoSSBP176S and EcoSSBF177C SSBs bind DNA similar to wildtype EcoSSB, indicating that the phenotype of ssb-113 is not an indication of altered DNA binding. The repair deficiency of either ssb-3 or ssb-113 strain can be complemented by overexpression of the respective other mutant.

About the article

Published Online: 2005-06-01

Published in Print: 2000-03-14

Citation Information: Biological Chemistry, Volume 381, Issue 3, Pages 183–192, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2000.025.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Alexander G. Kozlov, Min Kyung Shinn, and Timothy M. Lohman
Biophysical Journal, 2019
Min Kyung Shinn, Alexander G Kozlov, Binh Nguyen, Wlodek M Bujalowski, and Timothy M Lohman
Nucleic Acids Research, 2019
Richa Nigam, Monisha Mohan, Gururaj Shivange, Pranjal Kumar Dewangan, and Roy Anindya
Molecular Biology Reports, 2018
Hung-Yi Wu, Chih-Hao Lu, and Hung-Wen Li
Scientific Reports, 2017, Volume 7, Number 1
Alexander G. Kozlov, Min Kyung Shinn, Elizabeth A. Weiland, and Timothy M. Lohman
Journal of Molecular Biology, 2017, Volume 429, Number 18, Page 2790
Jin Inoue, Takayuki Nagae, Masaki Mishima, Yutaka Ito, Takehiko Shibata, and Tsutomu Mikawa
Journal of Biological Chemistry, 2011, Volume 286, Number 8, Page 6720
Christine Petzold, Aimee H. Marceau, Katherine H. Miller, Susan Marqusee, and James L. Keck
Journal of Biological Chemistry, 2015, Volume 290, Number 23, Page 14626
Sarah R. Wessel, Aimee H. Marceau, Shawn C. Massoni, Ruobo Zhou, Taekjip Ha, Steven J. Sandler, and James L. Keck
Journal of Biological Chemistry, 2013, Volume 288, Number 24, Page 17569
Gali Arad, Ayal Hendel, Claus Urbanke, Ute Curth, and Zvi Livneh
Journal of Biological Chemistry, 2008, Volume 283, Number 13, Page 8274
Michael D. Hobbs, Akiko Sakai, and Michael M. Cox
Journal of Biological Chemistry, 2007, Volume 282, Number 15, Page 11058
Robert D. Shereda, Douglas A. Bernstein, and James L. Keck
Journal of Biological Chemistry, 2007, Volume 282, Number 26, Page 19247
Mothe Sreedhar Reddy, N. Guhan, and K. Muniyappa
Journal of Biological Chemistry, 2001, Volume 276, Number 49, Page 45959
Dmitry M. Baitin, Marielle C. Gruenig, and Michael M. Cox
Journal of Biological Chemistry, 2008, Volume 283, Number 21, Page 14198
Andrew F. Voter, Michael P. Killoran, Gene E. Ananiev, Scott A. Wildman, F. Michael Hoffmann, and James L. Keck
SLAS DISCOVERY: Advancing Life Sciences R&D, 2017, Page 247255521771200
Piero R. Bianco and Yuri L. Lyubchenko
Protein Science, 2017, Volume 26, Number 4, Page 638
Aimee H. Marceau, Douglas A. Bernstein, Brian W. Walsh, Walker Shapiro, Lyle A. Simmons, James L. Keck, and Sergey Korolev
PLoS ONE, 2013, Volume 8, Number 3, Page e58765
Piero R. Bianco, Sasheen Pottinger, Hui Yin Tan, Trong Nguyenduc, Kervin Rex, and Umesh Varshney
Protein Science, 2017, Volume 26, Number 2, Page 227
Piero R. Bianco
Progress in Biophysics and Molecular Biology, 2017, Volume 127, Page 111
Michael M. Cox
Critical Reviews in Biochemistry and Molecular Biology, 2007, Volume 42, Number 1, Page 41
Robert D. Shereda, Alexander G. Kozlov, Timothy M. Lohman, Michael M. Cox, and James L. Keck
Critical Reviews in Biochemistry and Molecular Biology, 2008, Volume 43, Number 5, Page 289
Tiago Dos Vultos, Olga Mestre, Tone Tonjum, and Brigitte Gicquel
FEMS Microbiology Reviews, 2009, Volume 33, Number 3, Page 471
Catherine Suski and Kenneth J. Marians
Molecular Cell, 2008, Volume 30, Number 6, Page 779
D. A. Bernstein, J. M. Eggington, M. P. Killoran, A. M. Misic, M. M. Cox, and J. L. Keck
Proceedings of the National Academy of Sciences, 2004, Volume 101, Number 23, Page 8575
E. K. Davydova and L. B. Rothman-Denes
Proceedings of the National Academy of Sciences, 2003, Volume 100, Number 16, Page 9250
Natalie Naue, Monika Beerbaum, Andrea Bogutzki, Peter Schmieder, and Ute Curth
Nucleic Acids Research, 2013, Volume 41, Number 8, Page 4507
Zishuo Cheng, Aimee Caillet, Binbin Ren, and Huangen Ding
FEBS Letters, 2012, Volume 586, Number 21, Page 3825
Kun-Wei Chan, Yi-Juan Lee, Chia-Hung Wang, Haimei Huang, and Yuh-Ju Sun
Journal of Molecular Biology, 2009, Volume 388, Number 3, Page 508
Robert A. Pugh, Yuyen Lin, Chelcie Eller, Haley Leesley, Isaac K.O. Cann, and Maria Spies
Journal of Molecular Biology, 2008, Volume 383, Number 5, Page 982
Liza Cubeddu and Malcolm F. White
Journal of Molecular Biology, 2005, Volume 353, Number 3, Page 507
Rahul Roy, Alexander G. Kozlov, Timothy M. Lohman, and Taekjip Ha
Journal of Molecular Biology, 2007, Volume 369, Number 5, Page 1244
Robert D. Shereda, Nicholas J. Reiter, Samuel E. Butcher, and James L. Keck
Journal of Molecular Biology, 2009, Volume 386, Number 3, Page 612
Serguei V. Kuznetsov, Alexander G. Kozlov, Timothy M. Lohman, and Anjum Ansari
Journal of Molecular Biology, 2006, Volume 359, Number 1, Page 55
Gregor Witte, Roman Fedorov, and Ute Curth
Biophysical Journal, 2008, Volume 94, Number 6, Page 2269
Javier Vernal, Viviane I. Serpa, Carolina Tavares, Emanuel M. Souza, Fábio O. Pedrosa, and Hernán Terenzi
Protein Expression and Purification, 2007, Volume 53, Number 1, Page 195
Atul Sharma, Ram G. Nitharwal, Bhupender Singh, Ashraf Dar, Santanu Dasgupta, and Suman K. Dhar
FEBS Journal, 2009, Volume 276, Number 2, Page 519
Christophe Rouillon and Malcolm F. White
Research in Microbiology, 2011, Volume 162, Number 1, Page 19
Paola Loguercio Polosa, Fiammetta Megli, Barbara Di Ponzio, Maria Nicola Gadaleta, Palmiro Cantatore, and Marina Roberti
Gene, 2002, Volume 286, Number 1, Page 113
Christian J. Rudolph, Amy L. Upton, Geoffrey S. Briggs, and Robert G. Lloyd
DNA Repair, 2010, Volume 9, Number 3, Page 210
Meiting Wu, Young-Jun Park, Els Pardon, Stewart Turley, Andrew Hayhurst, Junpeng Deng, Jan Steyaert, and Wim G.J. Hol
Journal of Structural Biology, 2011, Volume 174, Number 1, Page 124
Ruchika Sharma and Desirazu N. Rao
Journal of Molecular Biology, 2009, Volume 385, Number 5, Page 1375
Tao Wei, Songtao Zhang, Shanshan Zhu, Duohong Sheng, Jinfeng Ni, and Yulong Shen
Biochemical and Biophysical Research Communications, 2008, Volume 367, Number 3, Page 523
Seijiro Shioi, Toyoyuki Ose, Katsumi Maenaka, Mitsunori Shiroishi, Yoshito Abe, Daisuke Kohda, Tsutomu Katayama, and Tadashi Ueda
Biochemical and Biophysical Research Communications, 2005, Volume 326, Number 4, Page 766
Duo Lu, Angela R. Myers, Nicholas P. George, and James L. Keck
Nucleic Acids Research, 2011, Volume 39, Number 15, Page 6536
Hua Zhang, Yuxi Tian, Ziduo Liu, Feng Huang, Lihua Hu, and Zheng-Guo He
Biochemistry (Moscow), 2011, Volume 76, Number 6, Page 658
Christopher P. Toseland and Martin R. Webb
Methods, 2010, Volume 51, Number 3, Page 259
Juan Liu, Meerim Choi, Adam G. Stanenas, Alicia K. Byrd, Kevin D. Raney, Christopher Cohan, and Piero R. Bianco
Protein Science, 2011, Volume 20, Number 6, Page 1005
James H. Werner, Hong Cai, Richard A. Keller, and Peter M. Goodwin
Biophysical Journal, 2005, Volume 88, Number 2, Page 1403
Natalie Naue, Roman Fedorov, Andreas Pich, Dietmar J. Manstein, and Ute Curth
Nucleic Acids Research, 2011, Volume 39, Number 4, Page 1398
L'ubomír Tomáka, Jozef Nosek, and Blanka Kucejová
Biological Chemistry, 2001, Volume 382, Number 2
M. Landwehr, U. Curth, and C. Urbanke
Biological Chemistry, 2002, Volume 383, Number 9
D. Lu, D. A. Bernstein, K. A. Satyshur, and J. L. Keck
Proceedings of the National Academy of Sciences, 2010, Volume 107, Number 2, Page 633
Erika Scaltriti, Mariella Tegoni, Claudio Rivetti, Hélène Launay, Jean-Yves Masson, Alfonso H. Magadan, Denise Tremblay, Sylvain Moineau, Roberto Ramoni, Julie Lichière, Valérie Campanacci, Christian Cambillau, and Miguel Ortiz-Lombardía
Molecular Microbiology, 2009, Volume 73, Number 6, Page 1156
J. A. Buss, Y. Kimura, and P. R. Bianco
Nucleic Acids Research, 2008, Volume 36, Number 22, Page 7029
Savvas N. Savvides, Srinivasan Raghunathan, Klaus Fütterer, Alex G. Kozlov, Timothy M. Lohman, and Gabriel Waksman
Protein Science, 2004, Volume 13, Number 7, Page 1942
Paula Martinez, Irene Siegl-Cachedenier, Juana M. Flores, and Maria A. Blasco
Aging Cell, 2009, Volume 8, Number 1, Page 2
D. Lu and J. L. Keck
Proceedings of the National Academy of Sciences, 2008, Volume 105, Number 27, Page 9169
Jacqueline M. Gulbis, Steven L. Kazmirski, Jeff Finkelstein, Zvi Kelman, Mike O'Donnell, and John Kuriyan
European Journal of Biochemistry, 2004, Volume 271, Number 2, Page 439

Comments (0)

Please log in or register to comment.
Log in