Jump to ContentJump to Main Navigation
Show Summary Details
In This Section

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred

12 Issues per year


IMPACT FACTOR 2016: 3.273

CiteScore 2016: 3.01

SCImago Journal Rank (SJR) 2015: 1.607
Source Normalized Impact per Paper (SNIP) 2015: 0.751

Online
ISSN
1437-4315
See all formats and pricing
In This Section
Volume 381, Issue 9-10 (Sep 2000)

Issues

The Transgeneticists Toolbox: Novel Methods for the Targeted Modification of Eukaryotic Genomes

Jürgen Bode / Thomas Schlake / Michaela Iber / Dirk Schübeler / Jost Seibler / Evgeney Snezhkov / Lev Nikolaev
Published Online: 2005-07-05 | DOI: https://doi.org/10.1515/BC.2000.103

Abstract

Classical techniques for gene transfer into mammalian cells involve tedious screening procedures to identify transgenic clones or animals with the appropriate level and stability of expression or with the correct developmental patterns. These first generation technologies are clearly inadequate for complex genetic strategies by which gene regulation can be studied in its entire complexity. While site-specific insertions can principally be achieved by homologous recombination or by adapting the recombination apparatus from phages or yeast, these methods usually lack the required efficiency or they perturb expression patterns by the co-insertion of prokaryotic vector parts. Virtually all of these problems can be overcome by recombinase-mediated cassette exchange (RMCE) techniques which cleanly replace a resident cassette that is flanked by two hetero-specific recombination target sites for a second cassette with the analogous design, presented on a targeting vector. After illustrating the fundamentals of site-specific recombination by selected experiments, the authors (arranged in the chronological order of their contribution) will describe their efforts to develop RMCE into a method of wide applicability. Further developments that have been initiated utilizing the particular potential of the RMCE principle will be outlined.

About the article

Published Online: 2005-07-05

Published in Print: 2000-09-13


Citation Information: Biological Chemistry, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2000.103.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Soeren Turan, Junhua Qiao, Sally Madden, Craig Benham, Marina Kotz, Axel Schambach, and Juergen Bode
Gene, 2014, Volume 546, Number 2, Page 135
[2]
Soeren Turan, Christoph Zehe, Johannes Kuehle, Junhua Qiao, and Juergen Bode
Gene, 2013, Volume 515, Number 1, Page 1
[3]
Junhua Qiao, André Oumard, Wolfgang Wegloehner, and Juergen Bode
Journal of Molecular Biology, 2009, Volume 390, Number 4, Page 579
[4]
David A. Sorrell and Andreas F. Kolb
Biotechnology Advances, 2005, Volume 23, Number 7-8, Page 431
[5]
Larry D. Adams, Luke Choi, Hai Qing Xian, Aizhen Yang, Brian Sauer, Ling Wei, and David I. Gottlieb
Molecular Brain Research, 2003, Volume 110, Number 2, Page 220
[6]
[7]
Jesse R Raab, Jonathan Chiu, Jingchun Zhu, Sol Katzman, Sreenivasulu Kurukuti, Paul A Wade, David Haussler, and Rohinton T Kamakaka
The EMBO Journal, 2012, Volume 31, Number 2, Page 330
[8]
Ying Huang, Yan Li, Yu Gang Wang, Xin Gu, Yan Wang, and Bei Fen Shen
Journal of Immunological Methods, 2007, Volume 322, Number 1-2, Page 28
[9]
H.J Lipps, A.C.W Jenke, K Nehlsen, M.F Scinteie, I.M Stehle, and J Bode
Gene, 2003, Volume 304, Page 23
[10]
Martin A. Lema and Moises Burachik
Comparative Immunology, Microbiology and Infectious Diseases, 2009, Volume 32, Number 2, Page 163
[11]
Soeren Turan, Melanie Galla, Ellen Ernst, Junhua Qiao, Christine Voelkel, Bernhard Schiedlmeier, Christoph Zehe, and Juergen Bode
Journal of Molecular Biology, 2011, Volume 407, Number 2, Page 193
[12]
Jeff Jia Cheng Hou, Joe Codamo, Warren Pilbrough, Benjamin Hughes, Peter P Gray, and Trent P Munro
Journal of Chemical Technology & Biotechnology, 2011, Volume 86, Number 7, Page 895
[13]
Kai Schönig, David Kentner, Manfred Gossen, Tina Baldinger, Jun Miao, Katrin Welzel, Andreas Vente, Dusan Bartsch, and Hermann Bujard
Transgenic Research, 2011, Volume 20, Number 3, Page 709
[14]
Ted H.J. Kwaks and Arie P. Otte
Trends in Biotechnology, 2006, Volume 24, Number 3, Page 137
[15]
Alexandra Baer and Jürgen Bode
Current Opinion in Biotechnology, 2001, Volume 12, Number 5, Page 473
[16]
Masumi Taki, Jiro Matsushita, and Masahiko Sisido
ChemBioChem, 2006, Volume 7, Number 3, Page 425
[17]
Margaret C. M. Smith and Helena M. Thorpe
Molecular Microbiology, 2002, Volume 44, Number 2, Page 299
[18]
Vibha Srivastava and David Gidoni
In Vitro Cellular & Developmental Biology - Plant, 2010, Volume 46, Number 3, Page 219
[19]
R Loew, Y Meyer, K Kuehlcke, L Gama-Norton, D Wirth, H Hauser, S Stein, M Grez, S Thornhill, A Thrasher, C Baum, and A Schambach
Gene Therapy, 2010, Volume 17, Number 2, Page 272
[20]
André Oumard, Junhua Qiao, Thomas Jostock, Jiandong Li, and Juergen Bode
Cytotechnology, 2006, Volume 50, Number 1-3, Page 93
[21]
Hubert Denise, Graham H. Coombs, and Jeremy C. Mottram
FEMS Microbiology Letters, 2004, Volume 235, Number 1, Page 89
[22]
I. Weidenfeld, M. Gossen, R. Low, D. Kentner, S. Berger, D. Gorlich, D. Bartsch, H. Bujard, and K. Schonig
Nucleic Acids Research, 2009, Volume 37, Number 7, Page e50
[23]
Anne B Tolstrup, Torben P Frandsen, and Søren Bregenholt
Expert Opinion on Biological Therapy, 2006, Volume 6, Number 9, Page 905
[24]
Louise M. Barnes, Catherine M. Bentley, Nicola Moy, and Alan J. Dickson
Biotechnology and Bioengineering, 2007, Volume 96, Number 2, Page 337
[25]
Sergey B. Akopov, Vera M. Ruda, Vera V. Batrak, Anna S. Vetchinova, Igor P. Chernov, Lev G. Nikolaev, Jürgen Bode, and Eugene D. Sverdlov
Mammalian Genome, 2006, Volume 17, Number 10, Page 1042
[26]
Madiha Derouazi, Rachel Flaction, Philippe Girard, Maria de Jesus, Martin Jordan, and Florian M. Wurm
Biotechnology Letters, 2006, Volume 28, Number 6, Page 373
[27]
L-M Houdebine
Reproduction in Domestic Animals, 2005, Volume 40, Number 4, Page 269
[28]
Comparative and Functional Genomics, 2001, Volume 2, Number 3, Page 187
[29]
T. J. Phillips, J. K. Belknap, R. J. Hitzemann, K. J. Buck, C. L. Cunningham, and J. C. Crabbe
Genes, Brain and Behavior, 2002, Volume 1, Number 1, Page 14
[30]
Qiaoming Long, Kathy D. Shelton, Jill Lindner, Julie R. Jones, and Mark A. Magnuson
genesis, 2004, Volume 39, Number 4, Page 256
[31]
Louise M. Barnes, Catherine M. Bentley, and Alan J. Dickson
Biotechnology and Bioengineering, 2003, Volume 81, Number 6, Page 631
[32]
I.P. Chernov, S.B. Akopov, L.G. Nikolaev, and E.D. Sverdlov
Journal of Cellular Biochemistry, 2002, Volume 84, Number 3, Page 590

Comments (0)

Please log in or register to comment.
Log in