Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred

12 Issues per year


IMPACT FACTOR 2016: 3.273

CiteScore 2016: 3.01

SCImago Journal Rank (SJR) 2016: 1.679
Source Normalized Impact per Paper (SNIP) 2016: 0.800

Online
ISSN
1437-4315
See all formats and pricing
More options …
Volume 381, Issue 9-10

Issues

Thyroid Hormone Receptors Bind to an Element in the Connexin43 Promoter

Anke Stock / Helmut Sies
Published Online: 2005-07-05 | DOI: https://doi.org/10.1515/BC.2000.120

Abstract

Thyroid hormones stimulate gap junctional communication in rat liver WB-F344 epithelial cells, elevating connexin43 mRNA and protein levels. In the present work we analysed connexin43 expression in liver and heart samples from thyroid hormone-treated Wistar rats. Connexin43 mRNA was elevated 2.1-fold in rat liver samples as compared to controls, while there was no change in heart. Thyroid hormone response elements in the rat connexin43 promoter region were examined; a candidate sequence, including a binding site for ligand-dependent transcription factors, was identified at position −480 to −464. This putative regulatory element, rCx−480, contains a direct repeat structure separated by three base pairs (DR3-type element). In electrophoretic mobility shift assays using in vitro translated proteins, the rCx−480 element formed stronger complexes with thyroid hormone receptor α/retinoid X receptor α heterodimers than with vitamin D receptor/retinoid X receptor α heterodimers. In transfected Cos-7 cells, promoter activation was observed via this element after treatment with 3,3′,5-triiodo-L-thyronine. Loss of binding was seen when the 3′ half-site or the spacer region of the rCx−480 element were experimentally mutated, while a stronger binding was observed with mutations introduced in the 5′ halfsite.

About the article

Published Online: 2005-07-05

Published in Print: 2000-09-13


Citation Information: Biological Chemistry, Volume 381, Issue 9-10, Pages 973–979, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2000.120.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
JUAN C. SÁEZ, VIVIANA M. BERTHOUD, MARÍA C. BRAÑES, AGUSTÍN D. MARTÍNEZ, and ERIC C. BEYER
Physiological Reviews, 2003, Volume 83, Number 4, Page 1359
[2]
David Martin, Thomas Tawadros, Laure Meylan, Amar Abderrahmani, Daniele F. Condorelli, Gérard Waeber, and Jacques-Antoine Haefliger
Journal of Biological Chemistry, 2003, Volume 278, Number 52, Page 53082
[3]
Rainer Schulz, Philipp Maximilian Görge, Anikó Görbe, Péter Ferdinandy, Paul D. Lampe, and Luc Leybaert
Pharmacology & Therapeutics, 2015, Volume 153, Page 90
[4]
Aida Salameh and Stefan Dhein
Biochimica et Biophysica Acta (BBA) - Biomembranes, 2005, Volume 1719, Number 1-2, Page 36
[5]
Masahito Oyamada, Yumiko Oyamada, and Tetsuro Takamatsu
Biochimica et Biophysica Acta (BBA) - Biomembranes, 2005, Volume 1719, Number 1-2, Page 6
[6]
Georges Pointis, Céline Fiorini, Norah Defamie, and Dominique Segretain
Biochimica et Biophysica Acta (BBA) - Biomembranes, 2005, Volume 1719, Number 1-2, Page 102
[7]
Katarzyna Marchlewska, Jolanta Slowikowska-Hilczer, Renata Walczak-Jedrzejowska, Elzbieta Oszukowska, Eliza Filipiak, and Krzysztof Kula
Journal of Experimental Zoology Part A: Ecological Genetics and Physiology, 2015, Volume 323, Number 4, Page 256
[8]
Constantinos Pantos and Iordanis Mourouzis
Oxidative Medicine and Cellular Longevity, 2014, Volume 2014, Page 1
[9]
Daniel G. Cyr
Spermatogenesis, 2011, Volume 1, Number 4, Page 325
[10]
Constantinos Pantos, Iordanis Mourouzis, and Dennis V. Cokkinos
Canadian Journal of Physiology and Pharmacology, 2012, Volume 90, Number 8, Page 977
[11]
Masahito Oyamada, Kumiko Takebe, and Yumiko Oyamada
Biochimica et Biophysica Acta (BBA) - Biomembranes, 2013, Volume 1828, Number 1, Page 118
[12]
Xiao-yan Jiang, Xiao-hong Feng, Guo-yan Li, Qian Zhao, and Hui-qing Yin
Acta Histochemica, 2010, Volume 112, Number 3, Page 278
[13]
Narcis Tribulova, Vladimir Knezl, Asher Shainberg, Shingo Seki, and Tomas Soukup
Vascular Pharmacology, 2010, Volume 52, Number 3-4, Page 102
[14]
Benjamin de Montgolfier, Aliou Faye, Céline Audet, and Daniel G. Cyr
General and Comparative Endocrinology, 2009, Volume 162, Number 3, Page 276
[15]
Benjamin de Montgolfier, Céline Audet, and Daniel G. Cyr
General and Comparative Endocrinology, 2011, Volume 170, Number 1, Page 110
[16]
Wusheng Xiao, Keyong Li, Qing Wu, Noriko Nishimura, Xiuli Chang, and Zhijun Zhou
Birth Defects Research Part B: Developmental and Reproductive Toxicology, 2009, Page n/a
[17]
Jerome Gilleron, Marielle Nebout, Linda Scarabelli, Francoise Senegas-Balas, Silvio Palmero, Dominique Segretain, and Georges Pointis
Journal of Cellular Physiology, 2006, Volume 209, Number 1, Page 153
[18]
Peng Yue, Yong Zhang, Zhimin Du, Jing Xiao, Zhenwei Pan, Ning Wang, Haiyan Yu, Wencai Ma, Hong Qin, Wen-Hui Wang, Dao-Hong Lin, and Baofeng Yang
Cellular Physiology and Biochemistry, 2006, Volume 17, Number 3-4, Page 129
[19]
Antonio De Maio, Virginia L. Vega, and Jorge E. Contreras
Journal of Cellular Physiology, 2002, Volume 191, Number 3, Page 269

Comments (0)

Please log in or register to comment.
Log in