Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred

IMPACT FACTOR 2017: 3.022

CiteScore 2018: 3.09

SCImago Journal Rank (SJR) 2018: 1.482
Source Normalized Impact per Paper (SNIP) 2018: 0.820

See all formats and pricing
More options …
Volume 381, Issue 9-10


Helical Tubes of FtsZ from Methanococcus jannaschii

Jan Löwe / Linda A. Amos
Published Online: 2005-07-05 | DOI: https://doi.org/10.1515/BC.2000.122


Bacterial cell division depends on the formation of a cytokinetic ring structure, the Z-ring. The bacterial tubulin homologue FtsZ is required for Z-ring formation. FtsZ assembles into various polymeric forms in vitro, indicating a structural role in the septum of bacteria. We have used recombinant FtsZ1 protein from M. jannaschii to produce helical tubes and sheets with high yield using the GTP analogue GMPCPP [guanylyl-(α,β)-methylene-diphosphate]. The sheets appear identical to the previously reported Ca++-induced sheets of FtsZ from M. jannaschii that were shown to consist of ‘thick’-filaments in which two protofilaments run in parallel. Tubes assembled either in Ca++ or in GMPCPP contain filaments whose dimensions indicate that they could be equivalent to the ‘thick’-filaments in sheets. Some tubes are hollow but others are filled by additional protein density. Helical FtsZ tubes differ from eukaryotic microtubules in that the filaments curve around the filament axis with a pitch of ~ 430 Å for Ca++-induced tubes or 590–620 Å for GMPCPP. However, their assembly in vitro as well-ordered polymers over distances comparable to the inner circumference of a bacterium may indicate a role in vivo. Their size and stability make them suitable for use in motility assays.

About the article

Published Online: 2005-07-05

Published in Print: 2000-09-13

Citation Information: Biological Chemistry, Volume 381, Issue 9-10, Pages 993–999, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2000.122.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Fenghui Guan, Jiayu Yu, Jie Yu, Yang Liu, Ying Li, Xin-Hua Feng, Kerwyn Casey Huang, Zengyi Chang, and Sheng Ye
eLife, 2018, Volume 7
Harald Preisner, Jörn Habicht, Sriram G. Garg, and Sven B. Gould
Cytoskeleton, 2018
Rachel Marrington, Elaine Small, Alison Rodger, Timothy R. Dafforn, and Stephen G. Addinall
Journal of Biological Chemistry, 2004, Volume 279, Number 47, Page 48821
Peter L. Ferguson and Gary S. Shaw
Journal of Biological Chemistry, 2004, Volume 279, Number 18, Page 18806
María A. Oliva, Sonia Huecas, Juan M. Palacios, Jaime Martín-Benito, José M. Valpuesta, and José M. Andreu
Journal of Biological Chemistry, 2003, Volume 278, Number 35, Page 33562
Senthil Arumugam, Zdeněk Petrášek, and Petra Schwille
Proceedings of the National Academy of Sciences, 2014, Volume 111, Number 13, Page E1192
D. Susanti, J. H. Wong, W. H. Vensel, U. Loganathan, R. DeSantis, R. A. Schmitz, M. Balsera, B. B. Buchanan, and B. Mukhopadhyay
Proceedings of the National Academy of Sciences, 2014, Volume 111, Number 7, Page 2608
J. Stricker, P. Maddox, E. D. Salmon, and H. P. Erickson
Proceedings of the National Academy of Sciences, 2002, Volume 99, Number 5, Page 3171
Vic Norris, Conrad Woldringh, and Eugenia Mileykovskaya
FEBS Letters, 2004, Volume 561, Number 1-3, Page 3
Harry H. Low, Martin C. Moncrieffe, and Jan Löwe
Journal of Molecular Biology, 2004, Volume 341, Number 3, Page 839
Stephen G. Addinall and Barry Holland
Journal of Molecular Biology, 2002, Volume 318, Number 2, Page 219
Jesús Mingorance, Germán Rivas, Marisela Vélez, Paulino Gómez-Puertas, and Miguel Vicente
Trends in Microbiology, 2010, Volume 18, Number 8, Page 348
Elaine Small, Rachel Marrington, Alison Rodger, David J. Scott, Katherine Sloan, David Roper, Timothy R. Dafforn, and Stephen G. Addinall
Journal of Molecular Biology, 2007, Volume 369, Number 1, Page 210
Bill Wickstead and Keith Gull
The Journal of Cell Biology, 2011, Volume 194, Number 4, Page 513
Tilman Läppchen, Victorine A. Pinas, Aloysius F. Hartog, Gerrit-Jan Koomen, Claudia Schaffner-Barbero, José Manuel Andreu, Daniel Trambaiolo, Jan Löwe, Aurélie Juhem, Andrei V. Popov, and Tanneke den Blaauwen
Chemistry & Biology, 2008, Volume 15, Number 2, Page 189
Harold P Erickson
Current Opinion in Cell Biology, 2001, Volume 13, Number 1, Page 55
Matthew T. Cabeen and Christine Jacobs-Wagner
Annual Review of Genetics, 2010, Volume 44, Number 1, Page 365
Katharine A. Michie and Jan Löwe
Annual Review of Biochemistry, 2006, Volume 75, Number 1, Page 467
Jan Löwe, Fusinita van den Ent, and Linda A. Amos
Annual Review of Biophysics and Biomolecular Structure, 2004, Volume 33, Number 1, Page 177
Jesús Mingorance, Sonsoles Rueda, Paulino Gómez-Puertas, Alfonso Valencia, and Miguel Vicente
Molecular Microbiology, 2001, Volume 41, Number 1, Page 83
Leigh G. Monahan, Andrew Robinson, and Elizabeth J. Harry
Molecular Microbiology, 2009, Volume 74, Number 4, Page 1004

Comments (0)

Please log in or register to comment.
Log in