Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred


IMPACT FACTOR 2018: 3.014
5-year IMPACT FACTOR: 3.162

CiteScore 2018: 3.09

SCImago Journal Rank (SJR) 2018: 1.482
Source Normalized Impact per Paper (SNIP) 2018: 0.820

Online
ISSN
1437-4315
See all formats and pricing
More options …
Volume 382, Issue 10

Issues

A Eubacterial Origin for the Human tRNA Nucleotidyltransferase?

A.S. Reichert / D.L. Thurlow / M. Mörl
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/BC.2001.176

Abstract

tRNA CCAtermini are generated and maintained by tRNA nucleotidyltransferases. Together with poly(A) polymerases and other enzymes they belong to the nucleotidyltransferase superfamily. However, sequence alignments within this family do not allow to distinguish between CCAadding enzymes and poly(A) polymerases. Furthermore, due to the lack of sequence information about animal CCAadding enzymes, identification of corresponding animal genes was not possible so far. Therefore, we looked for the human homolog using the bakers yeast tRNA nucleotidyltransferase as a query sequence in a BLAST search. This revealed that the human gene transcript CGI-47 (#AF151805) deposited in GenBank is likely to encode such an enzyme. To identify the nature of this protein, the cDNA of the transcript was cloned and the recombinant protein biochemically characterized, indicating that CGI-47 encodes a bona fide CCAadding enzyme and not a poly(A) polymerase. This confirmed animal CCAadding enzyme allowed us to identify putative homologs from other animals. Calculation of a neighborjoining tree, using an alignment of several CCAadding enzymes, revealed that the animal enzymes resemble more eubacterial ones than eukaryotic plant and fungal tRNA nucleotidyltransferases, suggesting that the animal nuclear cca genes might have been derived from the endosymbiotic progenitor of mitochondria and are therefore of eubacterial origin.

About the article

Published Online: 2005-06-01

Published in Print: 2001-10-15


Citation Information: Biological Chemistry, Volume 382, Issue 10, Pages 1431–1438, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2001.176.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Lieselotte Erber, Anne Hoffmann, Jörg Fallmann, Heike Betat, Peter F. Stadler, and Mario Mörl
RNA Biology, 2019, Page 1
[2]
Kamlesh Pawar, Megumi Shigematsu, Phillipe Loher, Shozo Honda, Isidore Rigoutsos, and Yohei Kirino
RNA Biology, 2019, Page 1
[3]
M. Leibovitch, N.E. Reid, J. Victoria, P.J. Hanic-Joyce, and P.B.M. Joyce
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2019, Volume 1867, Number 6, Page 616
[4]
Nathalie E. Reid, Judith S. Ngou, and Paul B.M. Joyce
Biochemical and Biophysical Research Communications, 2018
[5]
M. Leibovitch, P.J. Hanic-Joyce, and P.B.M. Joyce
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2018
[6]
Karolin Wellner, Heike Betat, and Mario Mörl
Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, 2018
[8]
Tasneem P. Sharma, Luke A. Wiley, S. Scott Whitmore, Kristin R. Anfinson, Cathryn M. Cranston, Douglas J. Oppedal, Heather T. Daggett, Robert F. Mullins, Budd A. Tucker, and Edwin M. Stone
Stem Cell Research, 2017, Volume 21, Page 58
[9]
Andreas Czech, Sandra Wende, Mario Mörl, Tao Pan, Zoya Ignatova, and Scott Blanchard
PLoS Genetics, 2013, Volume 9, Number 8, Page e1003767
[11]
Thalia Salinas-Giegé, Richard Giegé, and Philippe Giegé
International Journal of Molecular Sciences, 2015, Volume 16, Number 3, Page 4518
[12]
Mario Fiedler, Walter Rossmanith, Elmar Wahle, and Christiane Rammelt
Nucleic Acids Research, 2015, Page gkv891
[13]
Heike Betat, Tobias Mede, Sandy Tretbar, Lydia Steiner, Peter F. Stadler, Mario Mörl, and Sonja J. Prohaska
Nucleic Acids Research, 2015, Volume 43, Number 14, Page 6739
[14]
Felix G M Ernst, Christian Rickert, Alexander Bluschke, Heike Betat, Heinz-Jürgen Steinhoff, and Mario Mörl
RNA Biology, 2015, Volume 12, Number 4, Page 435
[15]
S. Wende, S. Bonin, O. Gotze, H. Betat, and M. Morl
Nucleic Acids Research, 2015, Volume 43, Number 11, Page 5617
[16]
Sandra Wende, Edward G. Platzer, Frank Jühling, Joern Pütz, Catherine Florentz, Peter F. Stadler, and Mario Mörl
Biochimie, 2014, Volume 100, Page 151
[17]
Matthew Leibovitch, Daniela Bublak, Pamela J. Hanic-Joyce, Bodo Tillmann, Nadine Flinner, Daniel Amsel, Klaus-Dieter Scharf, Oliver Mirus, Paul B. M. Joyce, and Enrico Schleiff
Biochemical Journal, 2013, Volume 453, Number 3, Page 401
[18]
Heike Betat, Christiane Rammelt, Georges Martin, and Mario Mörl
Molecular Cell, 2004, Volume 15, Number 3, Page 389
[19]
Oliver Rackham, Timothy R. Mercer, and Aleksandra Filipovska
Wiley Interdisciplinary Reviews: RNA, 2012, Volume 3, Number 5, Page 675
[20]
Louis Levinger, Isabel Oestreich, Catherine Florentz, and Mario Mörl
Journal of Molecular Biology, 2004, Volume 337, Number 3, Page 535
[21]
Martin A Augustin, Andreas S Reichert, Heike Betat, Robert Huber, Mario Mörl, and Clemens Steegborn
Journal of Molecular Biology, 2003, Volume 328, Number 5, Page 985
[22]
Walter Rossmanith
Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, 2012, Volume 1819, Number 9-10, Page 1017
[23]
Brian T. Keady, Kathleen R. Attfield, and Laura E. Hake
Biochemical and Biophysical Research Communications, 2002, Volume 297, Number 3, Page 573
[24]
Esther Lizano, Jens Schuster, Martin Müller, Janet Kelso, and Mario Mörl
Journal of Molecular Biology, 2007, Volume 366, Number 4, Page 1258
[25]
Marie Sissler, Bernard Lorber, Marie Messmer, André Schaller, Joern Pütz, and Catherine Florentz
Methods, 2008, Volume 44, Number 2, Page 176
[26]
Esther Lizano, Marion Scheibe, Christiane Rammelt, Heike Betat, and Mario Mörl
Biochimie, 2008, Volume 90, Number 5, Page 762
[27]
Sarah L. Zimmer, Aleks Schein, Gadi Zipor, David B. Stern, and Gadi Schuster
The Plant Journal, 2009, Volume 59, Number 1, Page 88
[28]
Pamela J. Hanic-Joyce and Paul B. M. Joyce
Yeast, 2002, Volume 19, Number 16, Page 1399

Comments (0)

Please log in or register to comment.
Log in