Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred

IMPACT FACTOR 2018: 3.014
5-year IMPACT FACTOR: 3.162

CiteScore 2018: 3.09

SCImago Journal Rank (SJR) 2018: 1.482
Source Normalized Impact per Paper (SNIP) 2018: 0.820

See all formats and pricing
More options …
Volume 382, Issue 10


Ferredoxins from the Archaeon Acidianus ambivalens: Overexpression and Characterization of the Non-Zinc-Containing Ferredoxin FdB

S. Janssen / J. Trincão / M. Teixeira / G. Schäfer / S. Anemüller
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/BC.2001.184


Two ferredoxin genes, fdA and fdB, from the extremely thermoacidophilic crenarchaeon Acidianus ambivalens have been sequenced; the sequences share 86% similarity. Whereas the deduced protein sequence of the ferredoxin FdA clearly contains a zincbinding motif, the corresponding sequence of the FdB is devoid of this motif. Thus far, only the zinccontaining ferredoxin, FdA, from A. ambivalens has been chemically and functionally characterized from its native source. Using RTPCR and Northern blot analysis, we show that both ferredoxins are expressed by A. ambivalens under either anaerobic or aerobic growth conditions. The zincfree ferredoxin, FdB, was overexpressed in E. coli and purified to homogeneity. Using EPR spectroscopy, we could demonstrate that FdB contains one [3Fe-4S]1+/0 and one [4Fe-4S]2+/1+ cluster. The reduction potential of the [3Fe-4S]1+/0 cluster was determined as 235±10 mV, at pH 6.5, by EPRmonitored redox titration. The high melting temperature of 108±2 C of FdB determined by CD spectroscopy reveals that it is not the binding of the Zn2+ that induces the extreme thermostability of these ferredoxins.

About the article

Published Online: 2005-06-01

Published in Print: 2001-10-15

Citation Information: Biological Chemistry, Volume 382, Issue 10, Pages 1501–1507, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2001.184.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Carlos Frazão, David Aragão, Ricardo Coelho, Sónia S. Leal, Cláudio M. Gomes, Miguel Teixeira, and Maria Arménia Carrondo
FEBS Letters, 2008, Volume 582, Number 5, Page 763
Susanne Griffin, Catherine L. Higgins, Tewfik Soulimane, and Pernilla Wittung-Stafshede
European Journal of Biochemistry, 2003, Volume 270, Number 23, Page 4736

Comments (0)

Please log in or register to comment.
Log in