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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred


IMPACT FACTOR 2018: 3.014
5-year IMPACT FACTOR: 3.162

CiteScore 2018: 3.09

SCImago Journal Rank (SJR) 2018: 1.482
Source Normalized Impact per Paper (SNIP) 2018: 0.820

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1437-4315
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Volume 382, Issue 10

Issues

Ferredoxins from the Archaeon Acidianus ambivalens: Overexpression and Characterization of the Non-Zinc-Containing Ferredoxin FdB

S. Janssen / J. Trincão / M. Teixeira / G. Schäfer / S. Anemüller
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/BC.2001.184

Abstract

Two ferredoxin genes, fdA and fdB, from the extremely thermoacidophilic crenarchaeon Acidianus ambivalens have been sequenced; the sequences share 86% similarity. Whereas the deduced protein sequence of the ferredoxin FdA clearly contains a zincbinding motif, the corresponding sequence of the FdB is devoid of this motif. Thus far, only the zinccontaining ferredoxin, FdA, from A. ambivalens has been chemically and functionally characterized from its native source. Using RTPCR and Northern blot analysis, we show that both ferredoxins are expressed by A. ambivalens under either anaerobic or aerobic growth conditions. The zincfree ferredoxin, FdB, was overexpressed in E. coli and purified to homogeneity. Using EPR spectroscopy, we could demonstrate that FdB contains one [3Fe-4S]1+/0 and one [4Fe-4S]2+/1+ cluster. The reduction potential of the [3Fe-4S]1+/0 cluster was determined as 235±10 mV, at pH 6.5, by EPRmonitored redox titration. The high melting temperature of 108±2 C of FdB determined by CD spectroscopy reveals that it is not the binding of the Zn2+ that induces the extreme thermostability of these ferredoxins.

About the article

Published Online: 2005-06-01

Published in Print: 2001-10-15


Citation Information: Biological Chemistry, Volume 382, Issue 10, Pages 1501–1507, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2001.184.

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