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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

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Volume 382, Issue 11 (Nov 2001)


Molecular Cloning and Biochemical Characterisation of Proteases from Staphylococcus epidermidis

Grzegorz Dubin / Dorota Chmiel / Pawel Mak / Magdalena Rakwalska / Malgorzata Rzychon / Adam Dubin
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/BC.2001.192


We report the complete coding sequence and the partial amino acid sequence (determined by chemical sequencing) of Staphylococcus epidermidis extracellular cysteine (Ecp) and serine (Esp) proteases. The first enzyme shows an extended sequence similarity to Staphylococcus aureus cysteine protease (staphopain) and the second one resembles the serine protease produced by that species. The region directly upstream of the sequence coding for the mature protein in both enzymes displays significant homology to the profragments encoded by sspB and sspA, respectively, thus suggesting that the characterised enzymes may also be produced as proproteins. Furthermore, we report some biological properties of the cysteine protease, contributing to a better understanding of its role as a possible virulence factor. The proteolytic activity of this enzyme was rapidly and efficiently inhibited by human α-2-macroglobulin; however, human kininogen as well as cystatins (A, C and D) were not inhibitory. Moreover, the protease was capable of inactivating, by limited proteolysis, both α-1-antitrypsin and HMWkininogen, but neither α-1-antichymotrypsin nor antithrombin III.

About the article

Published Online: 2005-06-01

Published in Print: 2001-11-13

Citation Information: Biological Chemistry, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2001.192. Export Citation

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