Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred


IMPACT FACTOR 2018: 3.014
5-year IMPACT FACTOR: 3.162

CiteScore 2018: 3.09

SCImago Journal Rank (SJR) 2018: 1.482
Source Normalized Impact per Paper (SNIP) 2018: 0.820

Online
ISSN
1437-4315
See all formats and pricing
More options …
Volume 382, Issue 2

Issues

Stimulation of Acid Sphingomyelinase Activity by Lysosomal Lipids and Sphingolipid Activator Proteins

Thomas Linke / Gundo Wilkening / Stephanie Lansmann / Heidi Moczall / Oliver Bartelsen / Judith Weisgerber / Konrad Sandhoff
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/BC.2001.035

Abstract

Acid sphingomyelinase is a watersoluble, lysosomal glycoprotein that catalyzes the degradation of membrane bound sphingomyelin into phosphorylcholine and ceramide. Sphingomyelin itself is an important component of the extracellular leaflet of various cellular membranes. The aim of the present investigation was to study sphingomyelin hydrolysis as a membrane bound process. We analyzed the degradation of sphingomyelin by recombinant, highly purified acid sphingomyelinase in a detergentfree, liposomal assay system. In order to mimic the in vivo intralysosomal conditions as closely as possible a number of negatively charged, lysosomally occuring lipids including bis(monoacylglycero)phosphate and phosphatidylinositol were incorporated into substratecarrying liposomes. Dolichol and its phosphate ester dolicholphosphate were also included in this study. Bis(monoacylglycero)phosphate and phosphatidylinositol were both effective stimulators of sphingomyelin hydrolysis. Dolichol and dolicholphosphate also significantly increased sphingomyelin hydrolysis. The influence of membrane curvature was investigated by incorporating the substrate into small (SUVs) and large unilamellar vesicles (LUVs) with varying mean diameter. Degradation rates were substantially higher in SUVs than in LUVs. Surface plasmon resonance experiments demonstrated that acid sphingomyelinase binds strongly to lipid bilayers. This interaction is significantly enhanced by anionic lipids such as bis(monoacylglycero)phosphate. Under detergent free conditions only the sphingolipid activator protein SAPC had a pronounced influence on sphingomyelin degradation in both neutral and negatively charged liposomes, catalyzed by highly purified acid sphingomyelinase, while SAPA, B and D had no noticeable effect on sphingomyelin degradation.

About the article

Published Online: 2005-06-01

Published in Print: 2001-02-12


Citation Information: Biological Chemistry, Volume 382, Issue 2, Pages 283–290, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2001.035.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Xueying Wang, Maximilian V. Schmitt, Lina Xu, Yupei Jiao, Lvjun Guo, Philip Lienau, Andreas Reichel, and Xiaohui Liu
Analytica Chimica Acta, 2019, Volume 1084, Page 60
[2]
Fernando Tobias, Koralege C. Pathmasiri, and Stephanie M. Cologna
Analytical and Bioanalytical Chemistry, 2019, Volume 411, Number 22, Page 5659
[3]
Russell W. Jenkins, Daniel Canals, and Yusuf A. Hannun
Cellular Signalling, 2009, Volume 21, Number 6, Page 836
[4]
Ambra Villani, Jørgen Benjaminsen, Christian Moritz, Katrin Henke, Jonas Hartmann, Nils Norlin, Kerstin Richter, Nicole L. Schieber, Tilman Franke, Yannick Schwab, and Francesca Peri
Developmental Cell, 2019, Volume 49, Number 1, Page 77
[5]
Mohammed H. Aldosari, Robert P. de Vries, Lucia R. Rodriguez, Nienke A. Hesen, Nataliia Beztsinna, André B.P. van Kuilenburg, Carla E.M. Hollak, Huub Schellekens, and Enrico Mastrobattista
European Journal of Pharmaceutics and Biopharmaceutics, 2019, Volume 137, Page 185
[6]
Zsolt Balogi, Gabriele Multhoff, Thomas Kirkegaard Jensen, Emyr Lloyd-Evans, Tetsumori Yamashima, Marja Jäättelä, John L. Harwood, and László Vígh
Progress in Lipid Research, 2019, Volume 74, Page 18
[7]
R. A. Dwek, T. D. Butters, F. M. Platt, T. M. Cox, Konrad Sandhoff, and Thomas Kolter
Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences, 2003, Volume 358, Number 1433, Page 847
[8]
Antoni Domagala, Klaudyna Fidyt, Malgorzata Bobrowicz, Joanna Stachura, Kacper Szczygiel, and Malgorzata Firczuk
International Journal of Molecular Sciences, 2018, Volume 19, Number 8, Page 2256
[10]
Jun-Xiang Bao, Min Xia, Justin L. Poklis, Wei-Qing Han, Christopher Brimson, and Pin-Lan Li
American Journal of Physiology-Heart and Circulatory Physiology, 2010, Volume 298, Number 3, Page H992
[11]
Wing-Kee Lee and Richard N. Kolesnick
Cellular Signalling, 2017, Volume 38, Page 134
[12]
Riia Plihtari, Eva Hurt-Camejo, Katariina Öörni, and Petri T. Kovanen
Journal of Lipid Research, 2010, Volume 51, Number 7, Page 1801
[13]
Misbaudeen Abdul-Hammed, Bernadette Breiden, Matthew A. Adebayo, Jonathan O. Babalola, Günter Schwarzmann, and Konrad Sandhoff
Journal of Lipid Research, 2010, Volume 51, Number 7, Page 1747
[14]
Susi Anheuser, Bernadette Breiden, Günter Schwarzmann, and Konrad Sandhoff
Journal of Lipid Research, 2015, Volume 56, Number 9, Page 1747
[15]
Vincent O. Oninla, Bernadette Breiden, Jonathan O. Babalola, and Konrad Sandhoff
Journal of Lipid Research, 2014, Volume 55, Number 12, Page 2606
[16]
Misbaudeen Abdul-Hammed, Bernadette Breiden, Günter Schwarzmann, and Konrad Sandhoff
Journal of Lipid Research, 2017, Volume 58, Number 3, Page 563
[17]
Hong Zhu, Tanihiro Yoshimoto, and Tetsumori Yamashima
Journal of Biological Chemistry, 2014, Volume 289, Number 40, Page 27432
[18]
Maria A. Pribasnig, Irina Mrak, Gernot F. Grabner, Ulrike Taschler, Oskar Knittelfelder, Barbara Scherz, Thomas O. Eichmann, Christoph Heier, Lukas Grumet, Jakob Kowaliuk, Matthias Romauch, Stefan Holler, Felix Anderl, Heimo Wolinski, Achim Lass, Rolf Breinbauer, Gunther Marsche, J. Mark Brown, and Robert Zimmermann
Journal of Biological Chemistry, 2015, Volume 290, Number 50, Page 29869
[19]
Youssef H. Zeidan and Yusuf A. Hannun
Journal of Biological Chemistry, 2007, Volume 282, Number 15, Page 11549
[20]
Stefan T. Hepbildikler, Roger Sandhoff, Melanie Kölzer, Richard L. Proia, and Konrad Sandhoff
Journal of Biological Chemistry, 2002, Volume 277, Number 4, Page 2562
[21]
Huawei Qiu, Tim Edmunds, Jennifer Baker-Malcolm, Kenneth P. Karey, Scott Estes, Cordula Schwarz, Heather Hughes, and Scott M. Van Patten
Journal of Biological Chemistry, 2003, Volume 278, Number 35, Page 32744
[22]
W.-K. Lee, S. Probst, M. P. Santoyo-Sánchez, W. Al-Hamdani, I. Diebels, J.-K. von Sivers, E. Kerek, E. J. Prenner, and F. Thévenod
Archives of Toxicology, 2017
[23]
Zhengtao Chu, Shadi Abu-Baker, Mary B. Palascak, Syed A. Ahmad, Robert S. Franco, Xiaoyang Qi, and Aamir Ahmad
PLoS ONE, 2013, Volume 8, Number 10, Page e75507
[24]
Alexander Simonis, Sabrina Hebling, Erich Gulbins, Sibylle Schneider-Schaulies, Alexandra Schubert-Unkmeir, and Christoph Tang
PLoS Pathogens, 2014, Volume 10, Number 6, Page e1004160
[25]
Omer F. Kuzu, Mesut Toprak, M. Anwar Noory, and Gavin P. Robertson
Pharmacological Research, 2017, Volume 117, Page 177
[26]
T. Kirkegaard, J. Gray, D. A. Priestman, K.-L. Wallom, J. Atkins, O. D. Olsen, A. Klein, S. Drndarski, N. H. T. Petersen, L. Ingemann, D. A. Smith, L. Morris, C. Bornaes, S. H. Jorgensen, I. Williams, A. Hinsby, C. Arenz, D. Begley, M. Jaattela, and F. M. Platt
Science Translational Medicine, 2016, Volume 8, Number 355, Page 355ra118
[27]
Roni Levin, Sergio Grinstein, and Johnathan Canton
Immunological Reviews, 2016, Volume 273, Number 1, Page 156
[28]
Zi-Jian Xiong, Jingjing Huang, Gennady Poda, Régis Pomès, and Gilbert G. Privé
Journal of Molecular Biology, 2016, Volume 428, Number 15, Page 3026
[29]
Tetsumori Yamashima
Ageing Research Reviews, 2016, Volume 32, Page 169
[31]
Onica LeGendre, Paul AS Breslin, and David A Foster
Molecular & Cellular Oncology, 2015, Volume 2, Number 4, Page e1006077
[32]
Shadi Abu-Baker, Zhengtao Chu, Ashley M. Stevens, Jie Li, and Xiaoyang Qi
Journal of Cancer Therapy, 2012, Volume 03, Number 04, Page 321
[33]
Thomas Kolter and Konrad Sandhoff
Biochimica et Biophysica Acta (BBA) - Biomembranes, 2006, Volume 1758, Number 12, Page 2057
[34]
Françoise Hullin-Matsuda, Tomohiko Taguchi, Peter Greimel, and Toshihide Kobayashi
Seminars in Cell & Developmental Biology, 2014, Volume 31, Page 48
[35]
Günter Schwarzmann, Christoph Arenz, and Konrad Sandhoff
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 2014, Volume 1841, Number 8, Page 1161
[36]
Heike Schulze and Konrad Sandhoff
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 2014, Volume 1841, Number 5, Page 799
[37]
Paul Saftig and Konrad Sandhoff
Nature, 2013, Volume 502, Number 7471, Page 312
[38]
Erich Gulbins and Richard N. Kolesnick
Cancer Cell, 2013, Volume 24, Number 3, Page 279
[39]
Bernadette Breiden and Konrad Sandhoff
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 2014, Volume 1841, Number 3, Page 441
[40]
T Kallunki, O D Olsen, and M Jäättelä
Oncogene, 2013, Volume 32, Number 16, Page 1995
[41]
Félix M Goñi and Alicia Alonso
FEBS Letters, 2002, Volume 531, Number 1, Page 38
[42]
Melanie Kölzer, Norbert Werth, and Konrad Sandhoff
FEBS Letters, 2004, Volume 559, Number 1-3, Page 96
[43]
Thomas Kolter and Konrad Sandhoff
FEBS Letters, 2010, Volume 584, Number 9, Page 1700
[44]
Félix M. Goñi, L.-Ruth Montes, and Alicia Alonso
Progress in Lipid Research, 2012, Volume 51, Number 3, Page 238
[45]
Nicolas Parent, Eric Winstall, Myriam Beauchemin, Claudie Paquet, Guy G. Poirier, and Richard Bertrand
Journal of Proteomics, 2009, Volume 72, Number 6, Page 960
[46]
Christian Sergelius, Sanna Niinivehmas, Terhi Maula, Mayuko Kurita, Shou Yamaguchi, Tetsuya Yamamoto, Shigeo Katsumura, Olli T. Pentikäinen, and J. Peter Slotte
Biochimica et Biophysica Acta (BBA) - Biomembranes, 2012, Volume 1818, Number 3, Page 474
[47]
Tetsumori Yamashima
Journal of Neurochemistry, 2012, Volume 120, Number 4, Page 477
[48]
F. Hullin-Matsuda, C. Luquain-Costaz, J. Bouvier, and I. Delton-Vandenbroucke
Prostaglandins, Leukotrienes and Essential Fatty Acids, 2009, Volume 81, Number 5-6, Page 313
[49]
Judith A. Hobert and Glyn Dawson
Biochemical and Biophysical Research Communications, 2007, Volume 358, Number 1, Page 111
[50]
Daniel Canals, David M Perry, Russell W Jenkins, and Yusuf A Hannun
British Journal of Pharmacology, 2011, Volume 163, Number 4, Page 694
[51]
Norma Marchesini and Yusuf A Hannun
Biochemistry and Cell Biology, 2004, Volume 82, Number 1, Page 27
[52]
Jun-Xiang Bao, Si Jin, Fan Zhang, Zheng-Chao Wang, Ningjun Li, and Pin-Lan Li
Antioxidants & Redox Signaling, 2010, Volume 12, Number 6, Page 703
[53]
[54]
M. Kölzer, C. Arenz, K. Ferlinz, N. Werth, H. Schulze, R. Klingenstein, and K. Sandhoff
Biological Chemistry, 2003, Volume 384, Number 9
[55]
Melanie Kölzer, Klaus Ferlinz, Oliver Bartelsen, Silvia Locatelli Hoops, Florian Lang, and Konrad Sandhoff
Biological Chemistry, 2004, Volume 385, Number 12
[56]
Jonathan O. Babalola, Michaela Wendeler, Bernadette Breiden, Christoph Arenz, Guenter Schwarzmann, Silvia Locatelli-Hoops, and Konrad Sandhoff
Biological Chemistry, 2007, Volume 388, Number 6
[57]
Hichem D. Gallala, Bernadette Breiden, and Konrad Sandhoff
Journal of Neurochemistry, 2011, Volume 116, Number 5, Page 702
[58]
Christina Tam, Vincent Idone, Cecilia Devlin, Maria Cecilia Fernandes, Andrew Flannery, Xingxuan He, Edward Schuchman, Ira Tabas, and Norma W. Andrews
The Journal of Cell Biology, 2010, Volume 189, Number 6, Page 1027
[59]
Thomas Kirkegaard, Anke G. Roth, Nikolaj H. T. Petersen, Ajay K. Mahalka, Ole Dines Olsen, Irina Moilanen, Alicja Zylicz, Jens Knudsen, Konrad Sandhoff, Christoph Arenz, Paavo K. J. Kinnunen, Jesper Nylandsted, and Marja Jäättelä
Nature, 2010, Volume 463, Number 7280, Page 549
[60]
Rebecca L. Rich and David G. Myszka
Journal of Molecular Recognition, 2002, Volume 15, Number 6, Page 352
[61]
Henriette Mozsolits and Marie-Isabel Aguilar
Biopolymers, 2002, Volume 66, Number 1, Page 3

Comments (0)

Please log in or register to comment.
Log in