Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred

IMPACT FACTOR 2018: 3.014
5-year IMPACT FACTOR: 3.162

CiteScore 2018: 3.09

SCImago Journal Rank (SJR) 2018: 1.482
Source Normalized Impact per Paper (SNIP) 2018: 0.820

See all formats and pricing

Access brought to you by:

provisional account

More options …
Volume 383, Issue 10


Three-State Equilibrium of Escherichia coli Trigger Factor

H. Patzelt / G. Kramer / T. Rauch / H.-J. Schönfeld / B. Bukau / E. Deuerling
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/BC.2002.182


Trigger Factor (TF) is the first chaperone that interacts with nascent chains of cytosolic proteins in Escherichia coli. Although its chaperone activity requires association with ribosomes, TF is present in vivo in a 2 3 fold molar excess over ribosomes and a fraction of it is not ribosomeassociated after cell lysis. Here we show that TF follows a threestate equilibrium. Size exclusion chromatography, crosslinking and analytical ultracentrifugation revealed that uncomplexed TF dimerizes with an apparent Kd of 18 M. Dimerization is mediated by the Nterminal ribosome binding domain and the Cterminal domain of TF, whereas the central peptidyl prolyl isomerase (PPIase) and substrate binding domain does not contribute to dimerization. Crosslinking experiments showed that TF is monomeric in its ribosomeassociated state. Quantitative analysis of TF binding to ribosomes revealed a dissociation constant for the TFribosome complex of approximately 1.2 M. From these data we estimate that in vivo most of the ribosomes are in complex with monomeric TF. Uncomplexed TF, however, is in a monomerdimer equilibrium with approximately two thirds of TF existing in a dimeric state.

About the article

Published Online: 2005-06-01

Published in Print: 2002-10-06

Citation Information: Biological Chemistry, Volume 383, Issue 10, Pages 1611–1619, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2002.182.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Günter Kramer, Ayala Shiber, and Bernd Bukau
Annual Review of Biochemistry, 2019, Volume 88, Number 1, Page 337
Dongjie Fan, Shunan Cao, Qiming Zhou, You Zhang, Lei Yue, Chang Han, Bo Yang, Yu Wang, Zhuo Ma, Lingxiang Zhu, and Chuanpeng Liu
The FASEB Journal, 2018, Volume 32, Number 12, Page 6655
Tomohide Saio, Soichiro Kawagoe, Koichiro Ishimori, and Charalampos G Kalodimos
eLife, 2018, Volume 7
Sebastian Hiller and Björn M. Burmann
Journal of Magnetic Resonance, 2018, Volume 289, Page 142
Leonor Morgado, Björn M. Burmann, Timothy Sharpe, Adam Mazur, and Sebastian Hiller
Nature Communications, 2017, Volume 8, Number 1
Shubhasis Haldar, Rafael Tapia-Rojo, Edward C. Eckels, Jessica Valle-Orero, and Julio M. Fernandez
Nature Communications, 2017, Volume 8, Number 1
Florian Wruck, Mario J. Avellaneda, Eline J. Koers, David P. Minde, Matthias P. Mayer, Günter Kramer, Alireza Mashaghi, and Sander J. Tans
Journal of Molecular Biology, 2017
Fabian Ries, Yvonne Carius, Marina Rohr, Karin Gries, Sandro Keller, C. Roy D. Lancaster, and Felix Willmund
Scientific Reports, 2017, Volume 7, Number 1
Frieder Merz, Anja Hoffmann, Anna Rutkowska, Beate Zachmann-Brand, Bernd Bukau, and Elke Deuerling
Journal of Biological Chemistry, 2006, Volume 281, Number 42, Page 31963
Anna Rutkowska, Matthias P. Mayer, Anja Hoffmann, Frieder Merz, Beate Zachmann-Brand, Christiane Schaffitzel, Nenad Ban, Elke Deuerling, and Bernd Bukau
Journal of Biological Chemistry, 2008, Volume 283, Number 7, Page 4124
Chuan-Peng Liu, Sarah Perrett, and Jun-Mei Zhou
Journal of Biological Chemistry, 2005, Volume 280, Number 14, Page 13315
Gottfried Eisner, Michael Moser, Ute Schäfer, Konstanze Beck, and Matthias Müller
Journal of Biological Chemistry, 2006, Volume 281, Number 11, Page 7172
Mehmet Tarik Can, Zeynep Kurkcuoglu, Gokce Ezeroglu, Arzu Uyar, Ozge Kurkcuoglu, Pemra Doruker, and L. Michel Espinoza-Fonseca
PLOS ONE, 2017, Volume 12, Number 4, Page e0176262
Kushagra Singhal, Jocelyne Vreede, Alireza Mashaghi, Sander J. Tans, Peter G. Bolhuis, and Emanuele Paci
PLoS ONE, 2013, Volume 8, Number 4, Page e59683
Feng Yang, Tai-Yen Chen, Łukasz Krzemiński, Ace George Santiago, Won Jung, and Peng Chen
Molecular Microbiology, 2016, Volume 102, Number 6, Page 992
Dongjie Fan, Lushan Liu, Lingxiang Zhu, Fang Peng, Qiming Zhou, and Chuanpeng Liu
Journal of Cellular Biochemistry, 2017, Volume 118, Number 1, Page 141
Dongjie Fan, Qiming Zhou, Chuanpeng Liu, and Jianzhong Zhang
Microbiological Research, 2016, Volume 193, Page 11
Annika Deckert, Christopher A. Waudby, Tomasz Wlodarski, Anne S. Wentink, Xiaolin Wang, John P. Kirkpatrick, Jack F. S. Paton, Carlo Camilloni, Predrag Kukic, Christopher M. Dobson, Michele Vendruscolo, Lisa D. Cabrita, and John Christodoulou
Proceedings of the National Academy of Sciences, 2016, Volume 113, Number 18, Page 5012
Marina V. Rodnina and Wolfgang Wintermeyer
Journal of Molecular Biology, 2016, Volume 428, Number 10, Page 2165
Gottfried Eisner, Hans-Georg Koch, Konstanze Beck, Joseph Brunner, and Matthias Müller
The Journal of Cell Biology, 2003, Volume 163, Number 1, Page 35
Elke Deuerling and Bernd Bukau
Critical Reviews in Biochemistry and Molecular Biology, 2004, Volume 39, Number 5-6, Page 261
Yogendra S. Rathore, Reema R. Dhoke, Maulik Badmalia, Amin Sagar, and Ashish
The Journal of Physical Chemistry B, 2015, Volume 119, Number 20, Page 6101
Anja Hoffmann, Bernd Bukau, and Günter Kramer
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2010, Volume 1803, Number 6, Page 650
Björn M. Burmann and Sebastian Hiller
Progress in Nuclear Magnetic Resonance Spectroscopy, 2015, Volume 86-87, Page 41
Gustavo A. Avila, Daniel H. Ramirez, Zacariah L. Hildenbrand, Pedro Jacquez, Susanna Chiocca, Jianjun Sun, German Rosas-Acosta, and Chuan Xiao
Protein Expression and Purification, 2015, Volume 105, Page 47
Thomas Bornemann, Wolf Holtkamp, and Wolfgang Wintermeyer
Nature Communications, 2014, Volume 5
T. Saio, X. Guan, P. Rossi, A. Economou, and C. G. Kalodimos
Science, 2014, Volume 344, Number 6184, Page 1250494
Felix Gloge, Annemarie H Becker, Günter Kramer, and Bernd Bukau
Current Opinion in Structural Biology, 2014, Volume 24, Page 24
I. Buskiewicz, E. Deuerling, S.-Q. Gu, J. Jockel, M. V. Rodnina, B. Bukau, and W. Wintermeyer
Proceedings of the National Academy of Sciences, 2004, Volume 101, Number 21, Page 7902
A. V. Ludlam, B. A. Moore, and Z. Xu
Proceedings of the National Academy of Sciences, 2004, Volume 101, Number 37, Page 13436
D. Baram, E. Pyetan, A. Sittner, T. Auerbach-Nevo, A. Bashan, and A. Yonath
Proceedings of the National Academy of Sciences, 2005, Volume 102, Number 34, Page 12017
Annemarie H Becker, Eugene Oh, Jonathan S Weissman, Günter Kramer, and Bernd Bukau
Nature Protocols, 2013, Volume 8, Number 11, Page 2212
Christopher A. Waudby, Hélène Launay, Lisa D. Cabrita, and John Christodoulou
Progress in Nuclear Magnetic Resonance Spectroscopy, 2013, Volume 74, Page 57
Andrew S. Thomas, Suifang Mao, and Adrian H. Elcock
Biophysical Journal, 2013, Volume 105, Number 3, Page 732
Yujin E. Kim, Mark S. Hipp, Andreas Bracher, Manajit Hayer-Hartl, and F. Ulrich Hartl
Annual Review of Biochemistry, 2013, Volume 82, Number 1, Page 323
Renuka Kudva, Kärt Denks, Patrick Kuhn, Andreas Vogt, Matthias Müller, and Hans-Georg Koch
Research in Microbiology, 2013, Volume 164, Number 6, Page 505
Georgeta L. Dumitru, Yvonne Groemping, Dagmar Klostermeier, Tobias Restle, Elke Deuerling, and Jochen Reinstein
Journal of Molecular Biology, 2004, Volume 339, Number 5, Page 1179
Eugene Oh, Annemarie H. Becker, Arzu Sandikci, Damon Huber, Rachna Chaba, Felix Gloge, Robert J. Nichols, Athanasios Typas, Carol A. Gross, Günter Kramer, Jonathan S. Weissman, and Bernd Bukau
Cell, 2011, Volume 147, Number 6, Page 1295
Erik Martinez-Hackert and Wayne A. Hendrickson
Cell, 2009, Volume 138, Number 5, Page 923
Dong-Jie Fan, Yan-Wei Ding, and Jun-Mei Zhou
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2009, Volume 1794, Number 6, Page 944
Dong-Jie Fan, Yan-Wei Ding, Xian-Ming Pan, and Jun-Mei Zhou
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2008, Volume 1784, Number 11, Page 1728
Chuan-Peng Liu and Jun-Mei Zhou
Biochemical and Biophysical Research Communications, 2004, Volume 313, Number 3, Page 509
Dibyendu Samanta, Anindita Das, Arpita Bhattacharya, Arunima Basu, Debasis Das, and Chanchal DasGupta
Biochemical and Biophysical Research Communications, 2009, Volume 384, Number 2, Page 137
Sucharita Dey, Arumay Pal, Pinak Chakrabarti, and Joël Janin
Journal of Molecular Biology, 2010, Volume 398, Number 1, Page 146
Jian-Yi Pan, Hongkai Wu, Xiang Liu, Pei-Pei Li, Hui Li, San-Ying Wang, and Xuan-Xian Peng
Molecular BioSystems, 2011, Volume 7, Number 9, Page 2651
J. Maillard, P. Genevaux, and C. Holliger
Microbiology, 2011, Volume 157, Number 8, Page 2410
Ole Kristensen and Michael Gajhede
Structure, 2003, Volume 11, Number 12, Page 1547
Li-Ling Zeng, Ling Yu, Zhen-Yu Li, Sarah Perrett, and Jun-Mei Zhou
Biochimie, 2006, Volume 88, Number 6, Page 613
Chuan-Peng Liu, Zhen-Yu Li, Guo-Chang Huang, Sarah Perrett, and Jun-Mei Zhou
Biochimie, 2005, Volume 87, Number 11, Page 1023
Amanda Raine, Ronald Ullers, Michael Pavlov, Joen Luirink, Jarl E.S Wikberg, and Måns Ehrenberg
Biochimie, 2003, Volume 85, Number 7, Page 659
Frank Schlünzen, Daniel N. Wilson, Pingsheng Tian, Jörg M. Harms, Stuart J. McInnes, Harly A.S. Hansen, Renate Albrecht, Jörg Buerger, Sigurd M. Wilbanks, and Paola Fucini
Structure, 2005, Volume 13, Number 11, Page 1685
Elizabeth A Craig, Helene C Eisenman, and Heather A Hundley
Current Opinion in Microbiology, 2003, Volume 6, Number 2, Page 157
Thomas Rauch, Heather A. Hundley, Chris Pfund, Renee D. Wegrzyn, William Walter, Günter Kramer, So-Young Kim, Elizabeth A. Craig, and Elke Deuerling
Molecular Microbiology, 2005, Volume 57, Number 2, Page 357
Florence Piette, Salvino D'Amico, Caroline Struvay, Gabriel Mazzucchelli, Jenny Renaut, Maria Luisa Tutino, Antoine Danchin, Pierre Leprince, and Georges Feller
Molecular Microbiology, 2010, Volume 76, Number 1, Page 120
Jamie P. Ellis, Peter H. Culviner, and Silvia Cavagnero
Protein Science, 2009, Volume 18, Number 10, Page 2003
Shang-Te Danny Hsu and Christopher M. Dobson
Biomolecular NMR Assignments, 2009, Volume 3, Number 1, Page 17
Yi Shi, Dong-Jie Fan, Shu-Xin Li, Hong-Jie Zhang, Sarah Perrett, and Jun-Mei Zhou
Protein Science, 2007, Volume 16, Number 6, Page 1165
Laurence C. Eisenlohr, Lan Huang, and Tania N. Golovina
Nature Reviews Immunology, 2007, Volume 7, Number 5, Page 403
Ada Yonath
Nature, 2006, Volume 444, Number 7118, Page 435
Christian M. Kaiser, Hung-Chun Chang, Vishwas R. Agashe, Sathish K. Lakshmipathy, Stephanie A. Etchells, Manajit Hayer-Hartl, F. Ulrich Hartl, and José M. Barral
Nature, 2006, Volume 444, Number 7118, Page 455
Lars Ferbitz, Timm Maier, Holger Patzelt, Bernd Bukau, Elke Deuerling, and Nenad Ban
Nature, 2004, Volume 431, Number 7008, Page 590

Comments (0)

Please log in or register to comment.
Log in