Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred

12 Issues per year

IMPACT FACTOR 2016: 3.273

CiteScore 2016: 3.01

SCImago Journal Rank (SJR) 2016: 1.679
Source Normalized Impact per Paper (SNIP) 2016: 0.800

See all formats and pricing
More options …
Volume 383, Issue 11 (Nov 2002)


Molecular and Immunological Characterization of Profilin from Mugwort Pollen

N. Wopfner / M. Willeroider / D. Hebenstreit / R. van Ree / M. Aalbers / P. Briza / J. Thalhamer / C. Ebner / K. Richter / F. Ferreira
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/BC.2002.199


In late summer in Europe, pollen of mugwort is one of the major sources of atopic allergens. No information about the complete molecular structure of any mugwort allergen has been published so far. Here we report the isolation and characterization of mugwort pollen cDNA clones coding for two isoforms of the panallergen profilin. Thirtysix percent of the mugwort allergic patients tested displayed IgE antibodies against natural and recombinant profilin, and no significant differences were observed in the IgEbinding properties of the isoforms. One profilin isoform was purified to homogeneity and detailed structural analysis indicated that the protein exists in solution as dimers and tetramers stabilized by sulfydryl and/or ionic interactions. Profilin monomers were detectable only after exposure of multimers to harsh denaturing conditions. Dimers and tetramers did not significantly differ in their ability to bind serum IgE from mugwort pollenallergic patients. However, oligomeric forms might have a higher allergenic potential than monomers because larger molecules would have additional epitopes for IgEmediated histamine release. Profilin isolated from mugwort pollen also formed multimers. Thus, oligomerization is not an artifact resulting from the recombinant production of the allergen. Inhibition experiments showed extensive IgE crossreactivity of recombinant mugwort profilin and profilin from various pollen and food extracts.

About the article

Published Online: 2005-06-01

Published in Print: 2002-11-13

Citation Information: Biological Chemistry, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2002.199.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Isabel Pablos, Stephanie Eichhorn, Peter Briza, Claudia Asam, Ulrike Gartner, Martin Wolf, Christof Ebner, Barbara Bohle, Naveen Arora, Stefan Vieths, Fatima Ferreira, and Gabriele Gadermaier
Scientific Reports, 2017, Volume 7, Number 1
Jose C. Jimenez-Lopez, María I. Rodríguez-García, Juan D. Alché, and Vladimir N. Uversky
PLoS ONE, 2013, Volume 8, Number 10, Page e76066
Jose C. Jimenez-Lopez, Sonia Morales, Antonio J. Castro, Dieter Volkmann, María I. Rodríguez-García, Juan de D. Alché, and Vladimir N. Uversky
PLoS ONE, 2012, Volume 7, Number 2, Page e30878
Israel Mares-Mejía, Siseth Martínez-Caballero, Claudia Garay-Canales, Patricia Cano-Sánchez, Alfredo Torres-Larios, Samuel Lara-González, Enrique Ortega, and Adela Rodríguez-Romero
Scientific Reports, 2016, Volume 6, Number 1
C.M. Landa-Pineda, A. Arroyo-Becerra, A. Rosas-Alvarado, L.M. Terán, M.L. Garcia-Cruz, L.A. Marchat, and C.A. Reyes-López
Allergologia et Immunopathologia, 2016, Volume 44, Number 2, Page 160
Gabriele Gadermaier, Michael Hauser, and Fatima Ferreira
Methods, 2014, Volume 66, Number 1, Page 55
J. Vitte and F. Bienvenu
EMC - Biologie Médicale, 2012, Volume 7, Number 3, Page 1
Richard W. Weber
Journal of Allergy and Clinical Immunology, 2003, Volume 112, Number 2, Page 229
Sofía Sirvent, Oscar Palomares, Javier Cuesta-Herranz, Mayte Villalba, and Rosalía Rodríguez
Journal of Agricultural and Food Chemistry, 2012, Volume 60, Number 23, Page 6011
Richard W. Weber
Annals of Allergy, Asthma & Immunology, 2007, Volume 99, Number 3, Page 203
Sofía Sirvent, Leticia Tordesillas, Mayte Villalba, Araceli Díaz-Perales, Javier Cuesta-Herranz, Gabriel Salcedo, and Rosalía Rodríguez
Annals of Allergy, Asthma & Immunology, 2011, Volume 106, Number 5, Page 429
Esther Compés, Elena Hernández, Santiago Quirce, Oscar Palomares, Rosalía Rodríguez, Javier Cuesta, Joaquín Sastre, and Mayte Villalba
Annals of Allergy, Asthma & Immunology, 2006, Volume 96, Number 4, Page 586
Plaimein Amnuaycheewa and Elvira Gonzalez de Mejia
Food Chemistry, 2010, Volume 119, Number 4, Page 1671
M.C. Arilla, I. Ibarrola, Y. Puente, J.C. Daza, A. Martínez, and J.A. Asturias
Molecular Immunology, 2007, Volume 44, Number 15, Page 3653
Beatrice Jahn-Schmid, Gottfried F. Fischer, Barbara Bohle, Ingrid Faé, Gabriele Gadermaier, Azra Dedic, Fatima Ferreira, and Christof Ebner
Journal of Allergy and Clinical Immunology, 2005, Volume 115, Number 2, Page 399
Eva Untersmayr and Erika Jensen-Jarolim
Pharmacology & Therapeutics, 2006, Volume 112, Number 3, Page 787
Demin Han, Xuxin Lai, Birgitte Gjesing, Nanshan Zhong, Luo Zhang, and Michael D. Spangfort
Acta Oto-Laryngologica, 2011, Volume 131, Number 5, Page 533
Rodrigo Barderas, Mayte Villalba, and Rosalía Rodríguez
Biological Chemistry, 2004, Volume 385, Number 8
Alexandra Santos and Ronald Van Ree
International Archives of Allergy and Immunology, 2011, Volume 155, Number 3, Page 191
S. Saarelainen, M. Rytkönen-Nissinen, J. Rouvinen, A. Taivainen, S. Auriola, A. Kauppinen, T. Kinnunen, and T. Virtanen
Clinical & Experimental Allergy, 2007, Volume 38, Number 2, Page 374
N. Wopfner, P. Gruber, M. Wallner, P. Briza, C. Ebner, A. Mari, K. Richter, L. Vogel, and F. Ferreira
Allergy, 2008, Volume 63, Number 7, Page 872
Richard W Weber
Current Opinion in Allergy and Clinical Immunology, 2005, Volume 5, Number 6, Page 563
Kannan Krishnan and Pierre D. J. Moens
Biophysical Reviews, 2009, Volume 1, Number 2, Page 71
G. Gadermaier, N. Wopfner, M. Wallner, M. Egger, A. Didierlaurent, G. Regl, F. Aberger, R. Lang, F. Ferreira, and T. Hawranek
Allergy, 2008, Volume 63, Number 11, Page 1543
Merima Bublin, Iris Lauer, Christina Oberhuber, Stefano Alessandri, Peter Briza, Christian Radauer, Martin Himly, Heimo Breiteneder, Stefan Vieths, and Karin Hoffmann-Sommergruber
Molecular Nutrition & Food Research, 2008
C. Oberhuber, Y. Ma, N. Wopfner, G. Gadermaier, A. Dedic, B. Niggemann, B. Maderegger, P. Gruber, F. Ferreira, O. Scheiner, and K. Hoffmann-Sommergruber
International Archives of Allergy and Immunology, 2008, Volume 145, Number 2, Page 94
Gabriele Gadermaier, Azra Dedic, Gerhard Obermeyer, Susanne Frank, Martin Himly, and Fatima Ferreira
Current Allergy and Asthma Reports, 2004, Volume 4, Number 5, Page 391
Richard W. Weber
Current Allergy and Asthma Reports, 2004, Volume 4, Number 5, Page 401
C. Wild, M. Wallner, K. Hufnagl, H. Fuchs, K. Hoffmann-Sommergruber, H. Breiteneder, O. Scheiner, F. Ferreira, and U. Wiedermann
Allergy, 2007, Volume 62, Number 1, Page 33
Y. Ma, L. Zuidmeer, B. Bohle, S.T.H. Bolhaar, G. Gadermaier, E. Gonzalez-Mancebo, M. Fernandez-Rivas, A.C. Knulst, M. Himly, R. Asero, C. Ebner, R. van Ree, F. Ferreira, H. Breiteneder, and K. Hoffmann-Sommergruber
Clinical Experimental Allergy, 2006, Volume 36, Number 8, Page 1087
C. Radauer, M. Willerroider, H. Fuchs, K. Hoffmann-Sommergruber, J. Thalhamer, F. Ferreira, O. Scheiner, and H. Breiteneder
Clinical Experimental Allergy, 2006, Volume 36, Number 7, Page 920
R. Asero, N. Wopfner, P. Gruber, G. Gadermaier, and F. Ferreira
Clinical Experimental Allergy, 2006, Volume 36, Number 5, Page 658
Yan Ma, Gabriele Gadermaier, Barbara Bohle, Suzanne Bolhaar, Andre Knulst, Zora Markovic-Housley, Heimo Breiteneder, Peter Briza, Karin Hoffmann-Sommergruber, and Fatima Ferreira
International Archives of Allergy and Immunology, 2005, Volume 139, Number 1, Page 53
Nicole Wopfner, Gabriele Gadermaier, Matthias Egger, Riccardo Asero, Christof Ebner, Beatrice Jahn-Schmid, and Fatima Ferreira
International Archives of Allergy and Immunology, 2005, Volume 138, Number 4, Page 337
Claudia Hoidn, Erika Puchner, Heidi Pertl, Elisabeth Holztrattner, and Gerhard Obermeyer
International Archives of Allergy and Immunology, 2005, Volume 137, Number 1, Page 27
S. Wagner, C. Radauer, C. Hafner, H. Fuchs, E. Jensen-Jarolim, B. Wuthrich, O. Scheiner, and H. Breiteneder
Clinical Experimental Allergy, 2004, Volume 34, Number 11, Page 1739
M. Lombardero, F. J. Garcia-Selles, F. Polo, L. Jimeno, M. J. Chamorro, G. Garcia-Casado, R. Sanchez-Monge, A. Diaz-Perales, G. Salcedo, and D. Barber
Clinical Experimental Allergy, 2004, Volume 34, Number 9, Page 1415
L. Jimeno, O. Duffort, C. Serrano, D. Barber, and F. Polo
Allergy, 2004, Volume 59, Number 9, Page 995

Comments (0)

Please log in or register to comment.
Log in