Jump to ContentJump to Main Navigation
Show Summary Details

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Turk, Boris / Wittinghofer, Alfred

12 Issues per year

IMPACT FACTOR 2015: 2.710
Rank 142 out of 289 in category Biochemistry & Molecular Biology in the 2015 Thomson Reuters Journal Citation Report/Science Edition

SCImago Journal Rank (SJR) 2015: 1.607
Source Normalized Impact per Paper (SNIP) 2015: 0.751
Impact per Publication (IPP) 2015: 2.609

See all formats and pricing



Select Volume and Issue


30,00 € / $42.00 / £23.00

Get Access to Full Text

Activation of c-Jun N-Terminal Kinase and Apoptosis in Endothelial Cells Mediated by Endogenous Generation of Hydrogen Peroxide

A. Ramachandran / D. Moellering / Y.-M. Go / S. Shiva / A.-L. Levonen / H. Jo / R.P. Patel / S. Parthasarathy / V.M. Darley-Usmar
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/BC.2002.071


Reactive oxygen species have been implicated in the activation of signal transduction pathways. However, extracellular addition of oxidants such as hydrogen peroxide (H2O2) often requires concentrations that cannot be readily achieved under physiological conditions to activate biological responses such as apoptosis. Explanations for this discrepancy have included increased metabolism of H2O2 in the extracellular environment and compartmentalization within the cell. We have addressed this issue experimentally by examining the induction of apoptosis of endothelial cells induced by exogenous addition of H2O2 and by a redox cycling agent, 2,3-dimethoxy 1,4-naphthoquinone, that generates H2O2 in cells. Here we show that low nanomolar steadystate concentrations (0.1 0.5 nmolmin 1106 cells) of H2O2 generated intracellularly activate cJun N terminal kinase and initiate apoptosis in endothelial cells. A comparison with bolus hydrogen peroxide suggests that the low rate of intracellular formation of this reactive oxygen species results in a similar profile of activation for both cJun N terminal kinase and the initiation of apoptosis. However, a detailed analysis reveals important differences in both the duration and profile for activation of these signaling pathways.

Published Online: 2005-06-01

Published in Print: 2002-04-12

Citation Information: Biological Chemistry. Volume 383, Issue 3-4, Pages 693–701, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2002.071, June 2005

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Ayako Yoshida, Susan G Elner, Zong-Mei Bian, Andrei L Kindezelskii, Howard R Petty, and Victor M Elner
Laboratory Investigation, 2003, Volume 83, Number 8, Page 1117
Yuan Wei, Yan Jinchuan, Liang Yi, Wu Jun, Wang Zhongqun, and Wang Cuiping
Inflammation, 2013, Volume 36, Number 3, Page 567
Brian P. Dranka, Gloria A. Benavides, Anne R. Diers, Samantha Giordano, Blake R. Zelickson, Colin Reily, Luyun Zou, John C. Chatham, Bradford G. Hill, Jianhua Zhang, Aimee Landar, and Victor M. Darley-Usmar
Free Radical Biology and Medicine, 2011, Volume 51, Number 9, Page 1621
Mihalis I. Panayiotidis, Sally P. Stabler, Robert H. Allen, Aglaia Pappa, and Carl W. White
Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 2009, Volume 674, Number 1-2, Page 23
Alberto Bindoli, Maria Pia Rigobello, Guido Scutari, Chiara Gabbiani, Angela Casini, and Luigi Messori
Coordination Chemistry Reviews, 2009, Volume 253, Number 11-12, Page 1692
Priya Ranjan, Vikas Anathy, Peter M. Burch, Kelly Weirather, J. David Lambeth, and Nicholas H. Heintz
Antioxidants & Redox Signaling, 2006, Volume 8, Number 9-10, Page 1447
Mashael Al-Mutairi, Sameer Al-Harthi, Laurence Cadalbert, and Robin Plevin
British Journal of Pharmacology, 2010, Volume 161, Number 4, Page 782
Meritxell Genesc??, Anna Sola, Daniel Azuara, Javier De Oca, and Georgina Hotter
Critical Care Medicine, 2005, Volume 33, Number 4, Page 827
David J. Smith, David J. Suggett, and Neil R. Baker
Global Change Biology, 2005, Volume 11, Number 1, Page 1

Comments (0)

Please log in or register to comment.