Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred


IMPACT FACTOR 2018: 3.014
5-year IMPACT FACTOR: 3.162

CiteScore 2018: 3.09

SCImago Journal Rank (SJR) 2018: 1.482
Source Normalized Impact per Paper (SNIP) 2018: 0.820

Online
ISSN
1437-4315
See all formats and pricing
More options …
Volume 383, Issue 3-4

Issues

Cysteine Supplementation Prevents Unweighting-Induced Ubiquitination in Association with Redox Regulation in Rat Skeletal Muscle

M. Ikemoto / T. Nikawa / M. Kano / K. Hirasaka / T. Kitano / C. Watanabe / R. Tanaka / T. Yamamoto / M. Kamada / K. Kishi
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/BC.2002.074

Abstract

We have previously reported that spaceflight and tail suspension enhanced degradation of rat myosin heavy chain (MHC) in association with activation of a ubiquitindependent proteolytic pathway [Ikemoto et al., FASEB J. 15 (2001), 1279 1281]. To elucidate whether the ubiquitination is accompanied by oxidative stress, we measured markers for oxidative stress, such as thiobarbituric acidreactive substance (TBARS) and glutathione disulfide (GSSG), in gastrocnemius muscle of tailsuspended rats. Glutathione (GSH) concentration in the muscle significantly decreased from day 5 and reached a minimum value on day 10. Tail suspension reciprocally increased concentrations of TBARS and GSSG in parallel with enhancement of protein ubiquitination, suggesting that oxidative stress may play an important role in protein ubiquitination caused by tail suspension. To prevent ubiquitination associated with oxidative stress, we also administered an antioxidative nutrient, cysteine, to tailsuspended rats. Intragastric supplementation of 140 mg/rat of cysteine for 2 weeks or longer normalized the ratio of GSH to GSSG in the muscle and suppressed protein ubiquitination and MHC fragmentation, compared with supplementation of the equimolar amount of alanine. The cysteine supplementation significantly suppressed the loss of hindlimb muscle mass. Our results suggest that supplementation of antioxidative nutrients, such as cysteine, may be beneficial for preventing ubiquitination of muscle proteins caused by unweighting.

About the article

Published Online: 2005-06-01

Published in Print: 2002-04-12


Citation Information: Biological Chemistry, Volume 383, Issue 3-4, Pages 715–721, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2002.074.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Rie Mukai and Junji Terao
The Journal of Physical Fitness and Sports Medicine, 2013, Volume 2, Number 4, Page 385
[2]
TOSHINORI YOSHIHARA, TAKAO SUGIURA, TSUBASA SIBAGUCHI, YUKI YAMAMOTO, KATSUMASA GOTO, TOMOMI ISOYAMA, HISASHI NAITO, DAIJIRO OHMORI, and TOSHITADA YOSHIOKA
Japanese Journal of Physical Fitness and Sports Medicine, 2010, Volume 59, Number 3, Page 303
[3]
Parco M. Siu, Emidio E. Pistilli, and Stephen E. Alway
Journal of Applied Physiology, 2008, Volume 105, Number 6, Page 1695
[4]
Hugues Magne, Isabelle Savary-Auzeloux, Didier Rémond, and Dominique Dardevet
Nutrition Research Reviews, 2013, Volume 26, Number 2, Page 149
[5]
Toshinori Yoshihara, Takao Sugiura, Tsubasa Shibaguchi, and Hisashi Naito
The Journal of Physical Fitness and Sports Medicine, 2019, Volume 8, Number 2, Page 61
[6]
Michael B. Reid
American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 2005, Volume 288, Number 6, Page R1423
[7]
Keith C. DeRuisseau, Andreas N. Kavazis, Melissa A. Deering, Darin J. Falk, Darin Van Gammeren, Tossaporn Yimlamai, George A. Ordway, and Scott K. Powers
Journal of Applied Physiology, 2005, Volume 98, Number 4, Page 1314
[8]
Jiancheng Yang, Gejing Zhang, Dandan Dong, and Peng Shang
International Journal of Molecular Sciences, 2018, Volume 19, Number 9, Page 2608
[9]
Takayuki Uchida, Yoshihiro Sakashita, Kanako Kitahata, Yui Yamashita, Chisato Tomida, Yuki Kimori, Akio Komatsu, Katsuya Hirasaka, Ayako Ohno, Reiko Nakao, Atsushi Higashitani, Akira Higashibata, Noriaki Ishioka, Toru Shimazu, Takeshi Kobayashi, Yuushi Okumura, Inho Choi, Motoko Oarada, Edward M. Mills, Shigetada Teshima-Kondo, Shin’ichi Takeda, Eiji Tanaka, Keiji Tanaka, Masahiro Sokabe, and Takeshi Nikawa
American Journal of Physiology-Cell Physiology, 2018, Volume 314, Number 6, Page C721
[10]
Tsubasa Shibaguchi, Yusuke Yamaguchi, Nobuyuki Miyaji, Toshinori Yoshihara, Hisashi Naito, Katsumasa Goto, Daijiro Ohmori, Toshitada Yoshioka, and Takao Sugiura
Physiological Reports, 2016, Volume 4, Number 15, Page e12885
[11]
Scott K. Powers, Andreas N. Kavazis, and Keith C. DeRuisseau
American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 2005, Volume 288, Number 2, Page R337
[12]
Parco M. Siu, Emidio E. Pistilli, and Stephen E. Alway
American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 2005, Volume 289, Number 4, Page R1015
[13]
Esther E. Dupont-Versteegden, Beau A. Strotman, Cathy M. Gurley, Dana Gaddy, Micheal Knox, James D. Fluckey, and Charlotte A. Peterson
American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 2006, Volume 291, Number 6, Page R1730
[14]
Ken Takahashi, Hiroki Okumura, Rui Guo, and Keiji Naruse
International Journal of Molecular Sciences, 2017, Volume 18, Number 7, Page 1426
[15]
Katsuya Hirasaka
Nippon Eiyo Shokuryo Gakkaishi, 2014, Volume 67, Number 6, Page 291
[16]
[17]
Tyler Barker and Maret G. Traber
The Journal of Physiology, 2008, Volume 586, Number 1, Page 309
[18]
Maria Antonietta Pellegrino, Jean-François Desaphy, Lorenza Brocca, Sabata Pierno, Diana Conte Camerino, and Roberto Bottinelli
The Journal of Physiology, 2011, Volume 589, Number 9, Page 2147
[19]
Jing Liu, Yunhua Peng, Zhihui Feng, Wen Shi, Lina Qu, Yinghui Li, Jiankang Liu, and Jiangang Long
Free Radical Biology and Medicine, 2014, Volume 69, Page 116
[20]
Keith C. DeRuisseau, R. Andrew Shanely, Nagabhavani Akunuri, Marc T. Hamilton, Darin Van Gammeren, A. Murat Zergeroglu, Michael McKenzie, and Scott K. Powers
American Journal of Respiratory and Critical Care Medicine, 2005, Volume 172, Number 10, Page 1267
[21]
Emilie Vazeille, Lamia Slimani, Agnès Claustre, Hugues Magne, Roland Labas, Daniel Béchet, Daniel Taillandier, Dominique Dardevet, Thierry Astruc, Didier Attaix, and Lydie Combaret
The Journal of Nutritional Biochemistry, 2012, Volume 23, Number 3, Page 245
[22]
Yuko Onishi, Katsuya Hirasaka, Ibuki Ishihara, Motoko Oarada, Jumpei Goto, Takayuki Ogawa, Naoto Suzue, Shunji Nakano, Harumi Furochi, Kazumi Ishidoh, Kyoichi Kishi, and Takeshi Nikawa
Biochemical and Biophysical Research Communications, 2005, Volume 336, Number 3, Page 799
[23]
Sergiy Sukhanov, Tadashi Yoshida, A. Michael Tabony, Yusuke Higashi, Sarah Galvez, Patrice Delafontaine, and Laura Semprun-Prieto
The American Journal of the Medical Sciences, 2011, Volume 342, Number 2, Page 143
[24]
Denis Breuillé, Fabienne Béchereau, Caroline Buffière, Philippe Denis, Corinne Pouyet, and Christiane Obled
Clinical Nutrition, 2006, Volume 25, Number 4, Page 634
[25]
Francesco Agostini, Luciano Dalla Libera, Jörn Rittweger, Sara Mazzucco, Mihaela Jurdana, Igor B. Mekjavic, Rado Pišot, Luisa Gorza, Marco Narici, and Gianni Biolo
The Journal of Physiology, 2010, Volume 588, Number 24, Page 5089
[26]
Takayuki Ogawa, Harumi Furochi, Mai Mameoka, Katsuya Hirasaka, Yuko Onishi, Naoto Suzue, Motoko Oarada, Motoki Akamatsu, Hiroshi Akima, Tetsuo Fukunaga, Kyoichi Kishi, Natsuo Yasui, Kazumi Ishidoh, Hideoki Fukuoka, and Takeshi Nikawa
Muscle & Nerve, 2006, Volume 34, Number 4, Page 463
[27]
Dalia Ismaeil Ibrahim Hemdan, Katsuya Hirasaka, Reiko Nakao, Shohei Kohno, Sachiko Kagawa, Tomoki Abe, Akiko Harada-Sukeno, Yuushi Okumura, Yutaka Nakaya, Junji Terao, and Takeshi Nikawa
The Journal of Medical Investigation, 2009, Volume 56, Number 1,2, Page 26
[28]
Gustavo BAJOTTO and Yoshiharu SHIMOMURA
Journal of Nutritional Science and Vitaminology, 2006, Volume 52, Number 4, Page 233
[29]
John M. Lawler, Wook Song, and Hyo-Bum Kwak
Muscle & Nerve, 2006, Volume 33, Number 2, Page 200
[30]
Katsuya Hirasaka, Takeshi Nikawa, Yuki Asanoma, Harumi Furochi, Yuko Onishi, Takayuki Ogawa, Naoto Suzue, Motoko Oarada, Toru Shimazu, and Kyoichi Kishi
Biological Sciences in Space, 2005, Volume 19, Number 1, Page 3
[31]
Madoka Ikemoto, Yoshihito Okamura, Mihoko Kano, Katsuya Hirasaka, Reiko Tanaka, Taeko Yamamoto, Takahiro Sasa, Takayuki Ogawa, Koichi Sairyo, Kyoichi Kishi, and Takeshi Nikawa
Journal of PHYSIOLOGICAL ANTHROPOLOGY and Applied Human Science, 2002, Volume 21, Number 5, Page 257

Comments (0)

Please log in or register to comment.
Log in