Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred

12 Issues per year

IMPACT FACTOR 2017: 3.022

CiteScore 2017: 2.81

SCImago Journal Rank (SJR) 2017: 1.562
Source Normalized Impact per Paper (SNIP) 2017: 0.705

See all formats and pricing
More options …
Volume 383, Issue 7-8


Cathepsin B Stability, But Not Activity, Is Affected in Cysteine:Cystine Redox Buffers

C. S. Pillay / C. Dennison
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/BC.2002.132


In order to test the hypothesis that the lysosomal cysteine protease cathepsin B may be redox regulated in vivo, cathepsin B activity and stability were measured in cysteine and/or cystinecontaining buffers. Cathepsin B activity in cysteinecontaining buffers was similar at pH 6.0 and pH 7.0, over all thiol concentrations tested. In contrast, the stability of the enzyme was greater at pH 6.0 than at pH 7.0. This suggests that the enzymes operational pH in vivo may be < pH 7.0. The activity of the enzyme was depressed in glutathionecontaining buffers. When assessed in cysteine:cystine redox buffers (pH 6.0 7.0) cathepsin B was active over a broad redox potential range, suggesting that cathepsin B activity may not be redox regulated. However, at pH 7.0, the stability of cathepsin B decreased with increasing reduction potential and ambient cystine concentration. This suggests that the stability of the enzyme at neutral pH is dependent on redox potential, and on the presence of oxidising agents.

About the article

Published Online: 2005-06-01

Published in Print: 2002-08-27

Citation Information: Biological Chemistry, Volume 383, Issue 7-8, Pages 1199–1204, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2002.132.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Pascal Clerc, Pauline Jeanjean, Nicolas Halalli, Michel Gougeon, Bernard Pipy, Julian Carrey, Daniel Fourmy, and Véronique Gigoux
Journal of Controlled Release, 2017
Ruben M. Markosyan, Chunhui Miao, Yi-Min Zheng, Gregory B. Melikyan, Shan-Lu Liu, Fredric S. Cohen, and Christopher F. Basler
PLOS Pathogens, 2016, Volume 12, Number 1, Page e1005373
Anke Klose, Paola Zigrino, Ralf Dennhöfer, Cornelia Mauch, and Nicolas Hunzelmann
Analytical Biochemistry, 2006, Volume 353, Number 1, Page 57

Comments (0)

Please log in or register to comment.
Log in