Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred

IMPACT FACTOR 2017: 3.022

CiteScore 2017: 2.81

SCImago Journal Rank (SJR) 2017: 1.562
Source Normalized Impact per Paper (SNIP) 2017: 0.705

See all formats and pricing
More options …
Volume 383, Issue 7-8


Aberrant Expression of Serpin Squamous Cell Carcinoma Antigen 2 in Human Tumor Tissues and Cell Lines: Evidence of Protection from Tumor Necrosis Factor-Mediated Apoptosis

A. Takeda / A. Kajiya / A. Iwasawa / Y. Nakamura / T. Hibino
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/BC.2002.136


Squamous cell carcinoma antigens (SCCA) 1 and 2 are highly homologous proteins of the serpin family, although they inhibit different types of proteinases. We investigated the expression of both SCCA mRNAs in tumor tissues, in various cell lines (A431, SAS, Ca9, HeLa, SKGIIIa, HSC-2, HSC-3, HSC-4 and KB) and in HSC cell lines in the presence of tumor necrosis factor α (TNFα). The expression of SCCA2 mRNA could be differentiated from that of SCCA1 in tumor tissues and cell lines by means of reverse transcriptionpolymerase chain reaction (RTPCR). The ratio between SCCA1 and SCCA2 mRNA expression showed selective expression of SCCA2 mRNA in SCC tissues from the uterine cervix compared to SCC tissues from the esophagus or skin. In addition, a significant level of SCCA2 mRNA expression was detected in the HSC-4 cell line, but not in Ca9, HeLa, SKGIIIa, or HSC-3 cells. In contrast, SCCA1 mRNA was detected in all samples tested. These results suggest that the level of expression of SCCA2 mRNA detected by RTPCR can be used to evaluate the status of SCC tumors. Next, we studied the effect of TNFα on SCCA1 and SCCA2 mRNA expression in HSC cell lines. SCCA1 mRNA expression was constantly increased in the three HSC cells examined with increasing time of exposure to TNFα. In contrast, SCCA2 mRNA expression was specific for HSC-4 cells. The survival rate of HSC-4 cells pretreated with TNFα (6.3 ng/ml) for 48 h was found to be 72%, compared with 42% and 9% for HSC-3 and HSC-2 cells, respectively, after apoptotic stimulation by TNFα (10 ng/ml) and cycloheximide (10 g/ml) for 18 h. Furthermore, selective expression of SCCA2 mRNA in HSC-4 pretreated with TNFα protected these cells from TNFαmediated apoptosis. Thus, SCCA2 overexpression in squamous tumor cells contributed to their survival by protecting them against TNFαinduced apoptosis.

About the article

Published Online: 2005-06-01

Published in Print: 2002-08-27

Citation Information: Biological Chemistry, Volume 383, Issue 7-8, Pages 1231–1236, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2002.136.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Jolanta Mrochem-Kwarciak, Tomasz Rutkowski, Andrzej Wygoda, Regina Deja, Agata Hajduk, and Krzysztof Składowski
Tumori Journal, 2018, Page tj.5000639
Mohamed H. Shamji, Jeff N. Temblay, Wei Cheng, Susan M. Byrne, Ellen Macfarlane, Amy R. Switzer, Natalia D.C. Francisco, Fedina Olexandra, Fabian Jacubczik, Stephen R. Durham, and Philip G. Ashton-Rickardt
Journal of Allergy and Clinical Immunology, 2017
Naohiro Nose, Masaaki Inoue, Mantaro Kodate, Takaaki Onituka, and Kosei Yasumoto
The Journal of the Japanese Association for Chest Surgery, 2006, Volume 20, Number 1, Page 40
Alessandra Biasiolo, Natascia Tono, Mariagrazia Ruvoletto, Santina Quarta, Cristian Turato, Gianmarco Villano, Luca Beneduce, Giorgio Fassina, Carlo Merkel, Angelo Gatta, Patrizia Pontisso, and Henrik Einwaechter
PLoS ONE, 2012, Volume 7, Number 7, Page e40658
Hsuan-Ho Chen, Hung-Ming Wang, Kang-Hsing Fan, Chien-Yu Lin, Tzu-Chen Yen, Chun-Ta Liao, I-How Chen, Chung-Jan Kang, Shiang-Fu Huang, and Jian-Xin Gao
PLoS ONE, 2013, Volume 8, Number 1, Page e55327
Zai-Shang Li, Kai Yao, Yong-Hong Li, Jie-Ping Chen, Chuang-Zhong Deng, Qi Zhao, Peng Chen, Bin Wang, Qi-Wu Mi, Zhuo-Wei Liu, Zi-Ke Qin, Hui Han, and Fang-Jian Zhou
BJU International, 2016, Volume 118, Number 2, Page 272
Mohamad Adel, Chung-Kan Tsao, Fu-Chan Wei, Huei-Tzu Chien, Chih-Hsiung Lai, Chun-Ta Liao, Hung-Ming Wang, Kang-Hsing Fan, Chung-Jan Kang, Joseph Tung-Chieh Chang, and Shiang-Fu Huang
Medicine, 2016, Volume 95, Number 14, Page e3149
Yoshinori Suminami, Fumio Kishi, Shugo Nawata, Akihiro Murakami, Yuko Sakaguchi, Kotaro Sueoka, Fumitaka Numa, Norihiro Sugino, and Hiroshi Kato
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 2005, Volume 1727, Number 3, Page 208
B. Singh, M. Haftek, and C.R. Harding
British Journal of Dermatology, 2014, Volume 171, Number 4, Page 760
Shiang-Fu Huang, Fu-Chan Wei, Chun-Ta Liao, Hung-Ming Wang, Chien-Yu Lin, Steven Lo, Jung-Ju Huang, I-How Chen, Chung-Jan Kang, Huei-Tzu Chien, and Hsuan-Ho Chen
Annals of Surgical Oncology, 2012, Volume 19, Number 12, Page 3856
Kyoung Ju Song, Hye-Jin Ahn, and Ho-Woo Nam
The Korean Journal of Parasitology, 2012, Volume 50, Number 1, Page 1
Tzu-Hui Li, Yi-Fen Kao, Chun-Chung Lui, and Wei-Hsi Chen
Clinical Biochemistry, 2006, Volume 39, Number 6, Page 662
Cristian Turato, Fiorella Calabrese, Alessandra Biasiolo, Santina Quarta, Mariagrazia Ruvoletto, Natascia Tono, Daniela Paccagnella, Giorgio Fassina, Carlo Merkel, Tim J Harrison, Angelo Gatta, and Patrizia Pontisso
Laboratory Investigation, 2010, Volume 90, Number 7, Page 1016
A. Torres, L. Storey, M. Anders, R.L. Miller, B.J. Bulbulian, J. Jin, S. Raghavan, J. Lee, H.B. Slade, and W. Birmachu
British Journal of Dermatology, 2007, Volume 157, Number 6, Page 1132
Markus Walz, Sabine Kellermann, Matilda Bylaite, Birgit Andrée, Ulrich Rüther, Ralf Paus, Jennifer E. Kloepper, Julia Reifenberger, and Thomas Ruzicka
Experimental Dermatology, 2007, Volume 16, Number 9, Page 715
Asifa S. Haider, Sara B. Peters, Helen Kaporis, Irma Cardinale, Ji Fei, Jurg Ott, Miki Blumenberg, Ann M. Bowcock, James G. Krueger, and John A. Carucci
Journal of Investigative Dermatology, 2006, Volume 126, Number 4, Page 869

Comments (0)

Please log in or register to comment.
Log in