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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred

12 Issues per year


IMPACT FACTOR 2016: 3.273

CiteScore 2016: 3.01

SCImago Journal Rank (SJR) 2016: 1.679
Source Normalized Impact per Paper (SNIP) 2016: 0.800

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ISSN
1437-4315
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Volume 383, Issue 7-8 (Aug 2002)

Issues

Purification and Characterization of the 20S Proteasome from Ostrich Skeletal Muscle

A. R. Thomas / V. Oosthuizen / R. J. Naudé / K. Muramoto
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/BC.2002.141

Abstract

The proteasome is a high molecular weight, multisubunit and multicatalytic enzyme. Here we report the purification and characterization of ostrich skeletal muscle 20S proteasome. It was purified to homogeneity with Mr 700 000, pI 6.67 and a ladder of 22.2 33.5 kDa bands on SDSPAGE. The amino acid composition and aminoterminal sequences showed large identities to those of other species. For the three major activities, pH and temperature optima ranged between 8.0 11.0 and 40 70C, and stabilities between 5 12 and up to 40 60C. Substrate specificity and inhibitory effects were also studied. Many similarities to other sources were shown, with a few significant differences.

About the article

Published Online: 2005-06-01

Published in Print: 2002-08-27


Citation Information: Biological Chemistry, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2002.141.

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