Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred

12 Issues per year

IMPACT FACTOR 2017: 3.022

CiteScore 2017: 2.81

SCImago Journal Rank (SJR) 2017: 1.562
Source Normalized Impact per Paper (SNIP) 2017: 0.705

See all formats and pricing
More options …
Volume 383, Issue 7-8


Purification and Characterization of the 20S Proteasome from Ostrich Skeletal Muscle

A. R. Thomas / V. Oosthuizen / R. J. Naudé / K. Muramoto
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/BC.2002.141


The proteasome is a high molecular weight, multisubunit and multicatalytic enzyme. Here we report the purification and characterization of ostrich skeletal muscle 20S proteasome. It was purified to homogeneity with Mr 700 000, pI 6.67 and a ladder of 22.2 33.5 kDa bands on SDSPAGE. The amino acid composition and aminoterminal sequences showed large identities to those of other species. For the three major activities, pH and temperature optima ranged between 8.0 11.0 and 40 70C, and stabilities between 5 12 and up to 40 60C. Substrate specificity and inhibitory effects were also studied. Many similarities to other sources were shown, with a few significant differences.

About the article

Published Online: 2005-06-01

Published in Print: 2002-08-27

Citation Information: Biological Chemistry, Volume 383, Issue 7-8, Pages 1267–1270, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2002.141.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Oliver Drews, Robert Wildgruber, Chenggong Zong, Ute Sukop, Mikkel Nissum, Gerhard Weber, Aldrin V. Gomes, and Peipei Ping
Molecular & Cellular Proteomics, 2007, Volume 6, Number 11, Page 2021
Julia R. Hayter, Mary K. Doherty, Colin Whitehead, Heather McCormack, Simon J. Gaskell, and Robert J. Beynon
Molecular & Cellular Proteomics, 2005, Volume 4, Number 9, Page 1370
Adele R. Thomas, Vaughan Oosthuizen, and Ryno J. Naudé
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2005, Volume 140, Number 3, Page 343
Adele R Thomas, Hatizivi Gondoza, Louwrens C Hoffman, Vaughan Oosthuizen, and Ryno J Naudé
Meat Science, 2004, Volume 67, Number 1, Page 113

Comments (0)

Please log in or register to comment.
Log in