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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Turk, Boris / Wittinghofer, Alfred


SCImago Journal Rank (SJR) 2015: 1.607
Source Normalized Impact per Paper (SNIP) 2015: 0.751
Impact per Publication (IPP) 2015: 2.609

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1437-4315
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DNA Polymerase β Gene Expression: The Promoter Activator CREB-1 Is Upregulated in Chinese Hamster Ovary Cells by DNA Alkylating Agent-Induced Stress

F. He / X.-P. Yang / D.K. Srivastava / S.H. Wilson

Citation Information: Biological Chemistry. Volume 384, Issue 1, Pages 19–23, ISSN (Print) 1431-6730, DOI: 10.1515/BC.2003.003, June 2005

Publication History

Published Online:
2005-06-01

Abstract

The DNA alkylating agent N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) upregulates the level of the base excision DNA repair enzyme DNA polymerase β (β-pol) in several mammalian cell types. Previous studies suggested that β-pol expression is upregulated via a transcriptional mechanism that requires: the specific cAMP response element (CRE) in the β-pol core promoter; a phosphorylated form of CREbinding protein-1 (CREB-1); and cellular protein kinase A activity. A large family of CRE-binding proteins, i.e., the ATF/CREB factors, has been identified in various cell types. This study further examines the role of CREbinding proteins in regulating β-pol expression through study of Chinese hamster ovary (CHO) cells. In CHO cell nuclear extract, CREB-1 and ATF-1 are the predominant CRE-binding protein family members recognizing the CRE in the β-pol core promoter. The concentration of CREB-1 increases strongly in CHO cells after exposure to MNNG. In contrast, the level of ATF-1 does not change after MNNG treatment. Recombinant expression of CREB-1 in CHO cells is sufficient to increase expression of the endogenous β-pol gene, even in the absence of MNNG exposure. These results indicate that β-pol gene expression in CHO cells can be upregulated by CREB-1 and that the activation of β-pol gene expression in response to DNA alkylating agent exposure involves a strong increase in the level of CREB-1.

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