Jump to ContentJump to Main Navigation
Show Summary Details

Buchner, Johannes

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Turk, Boris / Wittinghofer, Alfred

SCImago Journal Rank (SJR) 2015: 1.607
Source Normalized Impact per Paper (SNIP) 2015: 0.751
Impact per Publication (IPP) 2015: 2.609

See all formats and pricing



Select Volume and Issue


30,00 € / $42.00 / £23.00

Get Access to Full Text

Internalisation and Degradation of Histatin 5 by Candida albicans

A.L.A. Ruissen / J. Groenink / P. Krijtenberg / E. Walgreen-Weterings / W. van 't Hof / E.C.I. Veerman / A.V. Nieuw Amerongen

Citation Information: Biological Chemistry. Volume 384, Issue 1, Pages 183–190, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2003.020, June 2005

Publication History

Published Online:


Histatins, salivary antimicrobial peptides, are susceptible to proteolytic degradation, often ascribed to host proteinases. In this study, we addressed the question whether proteolytic activity from microbial sources can contribute to this degradation. Candida albicans, an opportunistic yeast that is susceptible to the histatins, was used as target organism. The most potent histatin (histatin 5: sequence: DSHAKRHHGYKRKFHEKHHSHRGY), two histatin 5 fragments (dh-5: sequence: KRKFHEKHHSHRGY; P-113: sequence: AKRHHGYKRKFH) and an all-D isomer of the latter (P-113D) were used as model peptides. All L peptides were susceptible to degradation by C. albicans. Cleavage was established at Lys5 and His19 of histatin 5, Lys11, Arg12, Phe14, Glu16, Lys17, His18 and Ser20 of dh-5 and Ala4 and Lys11 of P-113. In addition, it was found that secreted C. albicans enzymes are not involved in the degradation process and that blocking cell entry of the peptides greatly impedes degradation. Moreover, P-113D, which is biologically as active as P-113, was hardly susceptible to proteolysis. These data imply that proteolysis occurs mainly intracellularly and is not used as a protective mechanism against histatin activity. Together, our results suggest that, besides host proteinases, microbial enzymes play an important role in histatin degradation.

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

S. H. Shin, Y. S. Lee, Y. P. Shin, B. Kim, M. H. Kim, H.-R. Chang, W. S. Jang, and I. H. Lee
Journal of Antimicrobial Chemotherapy, 2013, Volume 68, Number 5, Page 1152
Elżbieta Kamysz, Emilia Sikorska, Anna Karafova, and Małgorzata Dawgul
Journal of Peptide Science, 2012, Volume 18, Number 9, Page 560
Lijun Huo, Kai Zhang, Junqi Ling, Zhixiang Peng, Xiangya Huang, Hongyan Liu, and Lisha Gu
Archives of Oral Biology, 2011, Volume 56, Number 9, Page 869
Jiří Dostál, Helena Dlouhá, Petr Maloň, Iva Pichová, and Olga Hrušková-Heidingsfeldová
Biological Chemistry, 2005, Volume 386, Number 8

Comments (0)

Please log in or register to comment.