Jump to ContentJump to Main Navigation
Show Summary Details

Buchner, Johannes

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Turk, Boris / Wittinghofer, Alfred

SCImago Journal Rank (SJR) 2015: 1.607
Source Normalized Impact per Paper (SNIP) 2015: 0.751
Impact per Publication (IPP) 2015: 2.609

See all formats and pricing



Select Volume and Issue


30,00 € / $42.00 / £23.00

Get Access to Full Text

Semisynthesis and Application of Carboxyfluorescein-Labelled Biologically Active Human Interleukin-8

R. David / Z. Machova / A. G. Beck-Sickinger

Citation Information: Biological Chemistry. Volume 384, Issue 12, Pages 1619–1630, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2003.180, June 2005

Publication History

Published Online:


Human interleukin 8 (hIL-8), a neutrophil-activating and chemotactic cytokine, is known to play an important role in the pathogenesis of a large number of neutrophil-driven inflammatory diseases. This cytokine belongs to the family of CXC chemokines, mediating the response through binding to the seventransmembrane helical G protein-coupled receptors CXCR1 and CXCR2. For the first time, we employed the expressed protein ligation (EPL) strategy to chemokine synthesis and subsequent modification. The ligation site was chosen with respect to the position of four cysteine residues within the hIL-8 sequence. Ligation with synthetic peptides that carry cysteine at their N-termini resulted in fulllength hIL-8 and the specifically carboxyfluoresceinlabelled analogue [K69(CF)]hIL-8(1 77). [K69(CF)]hIL-8(1-77) was fully active as shown by inhibition of cAMP production. Furthermore, this analogue was used to study receptor internalisation in human promyelotic HL60 cells that express CXCR1 and CXCR2 receptors. Binding and quenching studies were performed on HL60 membranes and suggest that the Cterminus of IL-8 is accessible to solvent in the receptor-bound state. Thus, we introduce here a powerful approach that allows the site-specific incorporation of chemical modifications into the sequence of chemokines, which opens new avenues for studying IL-8 function.

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Thomas Durek and Christian F.W. Becker
Biomolecular Engineering, 2005, Volume 22, Number 5-6, Page 153
Annette G Beck-Sickinger and Nydia Panitz
Current Opinion in Chemical Biology, 2014, Volume 22, Page 100
Max Steinhagen, Katja Zunker, Karoline Nordsieck, and Annette G. Beck-Sickinger
Bioorganic & Medicinal Chemistry, 2013, Volume 21, Number 12, Page 3504
Karoline Nordsieck, Annelie Pichert, Sergey A. Samsonov, Lars Thomas, Christian Berger, M. Teresa Pisabarro, Daniel Huster, and Annette G. Beck-Sickinger
ChemBioChem, 2012, Volume 13, Number 17, Page 2558
Ana P. Esteves, Lígia M. Rodrigues, Marília E. Silva, Sanjay Gupta, Ana M.F. Oliveira-Campos, Oldrich Machalicky, and António J. Mendonça
Tetrahedron, 2005, Volume 61, Number 36, Page 8625
Michael P.O. Richter, Kai Holland-Nell, and Annette G. Beck-Sickinger
Tetrahedron, 2004, Volume 60, Number 35, Page 7507
A. Pichert, S. A. Samsonov, S. Theisgen, L. Thomas, L. Baumann, J. Schiller, A. G. Beck-Sickinger, D. Huster, and M. T. Pisabarro
Glycobiology, 2012, Volume 22, Number 1, Page 134
Claire Jones, Jonas Denecke, Ronald Sträter, Torsten Stölting, Yvonne Schunicht, Dagmar Zeuschner, Judith Klumperman, Dirk J. Lefeber, Oliver Spelten, Alexander Zarbock, Sørge Kelm, Karen Strenge, Stuart M. Haslam, Kerstin Lühn, Dorothea Stahl, Luca Gentile, Thomas Schreiter, Philip Hilgard, Annette G. Beck-Sickinger, Thorsten Marquardt, and Martin K. Wild
The American Journal of Pathology, 2011, Volume 179, Number 4, Page 1969
Dana Wiese and Katja Schmitz
Journal of Immunological Methods, 2011, Volume 364, Number 1-2, Page 77
Lars Baumann and Annette G. Beck-Sickinger
Biopolymers, 2010, Volume 94, Number 6, Page 771
Kathrin Bellmann-Sickert, Lars Baumann, and Annette G. Beck-Sickinger
Journal of Peptide Science, 2010, Volume 16, Number 10, Page 568
Ralf David, Michael P.O. Richter, and Annette G. Beck-Sickinger
European Journal of Biochemistry, 2004, Volume 271, Number 4, Page 663
Madhukar N. Jachak, Sandeep M. Bagul, Dilip R. Birari, Maruti G. Ghagare, Muddassar A. Kazi, Raghunath B. Toche, and Vijayavitthal T. Mathad
Journal of Fluorescence, 2010, Volume 20, Number 3, Page 787
J. Scheller, N. Ohnesorge, and S. Rose-John
Scandinavian Journal of Immunology, 2006, Volume 63, Number 5, Page 321

Comments (0)

Please log in or register to comment.