Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred


IMPACT FACTOR 2017: 3.022

CiteScore 2018: 3.09

SCImago Journal Rank (SJR) 2018: 1.482
Source Normalized Impact per Paper (SNIP) 2018: 0.820

Online
ISSN
1437-4315
See all formats and pricing
More options …
Volume 384, Issue 3

Issues

Hepatitis B Virus Core Antigen: Enhancement of Its Production in Escherichia coli, and Interaction of the Core Particles with the Viral Surface Antigen

W.S. Tan / M.R. Dyson / K. Murray
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/BC.2003.042

Abstract

The core antigen (HBcAg) of hepatitis B Virus (HBV) can be expressed in Escherichia coli where it assembles into icosahedral particles containing 240 or 180 subunits. Analysis of the two kinds of particles by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) showed that a substantial proportion of their subunits were smaller than the full-length HBcAg monomer and of variable size, but all had the same N-terminal sequence showing that the smaller species were heterogeneous in their arginine-rich C-terminal regions. Around 50% of these arginine residues are encoded by the triplet AGA which is rare in E. coli. Supplementation of the level of AGA tRNA in the cell by transformation with plasmids expressing the T4 AGA tRNA gene significantly enhanced the yield of HBcAg. Fusion phage carrying a ligand specific for HBcAg showed no significant difference in the affinity for the two sizes of HBcAg particles, but in similar reactions in solution HBV surface antigen exhibited differential affinities for the same two HBcAg preparations.

About the article

Published Online: 2005-06-01

Published in Print: 2003-03-14


Citation Information: Biological Chemistry, Volume 384, Issue 3, Pages 363–371, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2003.042.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[2]
Priadarishni Ramanujam, Wen Siang Tan, Sheila Nathan, and Khatijah Yusoff
BioTechniques, 2004, Volume 36, Number 2, Page 296
[3]
Roya Biabanikhankahdani, Kok Ho, Noorjahan Alitheen, and Wen Tan
Nanomaterials, 2018, Volume 8, Number 4, Page 236
[4]
Mohamad Farid Abd Muain, Kooi Hoong Cheo, Muhamad Nadzmi Omar, Amir Syahir Amir Hamzah, Hong Ngee Lim, Abu Bakar Salleh, Wen Siang Tan, and Asilah Ahmad Tajudin
Bioelectrochemistry, 2018
[5]
Hon Wei Ng, Micky Fu Xiang Lee, Gek Kee Chua, Bee Koon Gan, Wen Siang Tan, Chien Wei Ooi, Siah Ying Tang, Eng Seng Chan, and Beng Ti Tey
Journal of Separation Science, 2018
[6]
Swee Lu Lim, Hon Wei Ng, Made Angga Akwiditya, Chien-Wei Ooi, Eng-Seng Chan, Kok Lian Ho, Wen Siang Tan, Gek Kee Chua, and Beng Ti Tey
Process Biochemistry, 2018
[7]
Frank Gillam, Jianqiang Zhang, and Chenming Zhang
Journal of Virological Methods, 2017
[8]
Roya Biabanikhankahdani, Noorjahan Banu Mohamed Alitheen, Kok Lian Ho, and Wen Siang Tan
Scientific Reports, 2016, Volume 6, Number 1
[9]
Azira Muhamad, Kok Lian Ho, Mohd. Basyaruddin Abdul Rahman, Bimo A. Tejo, Dušan Uhrín, and Wen Siang Tan
Org. Biomol. Chem., 2015, Volume 13, Number 28, Page 7780
[10]
S.H.S. Mariam, C.W. Ooi, W.S. Tan, O.A. Janna, A. Arbakariya, and B.T. Tey
Separation and Purification Technology, 2015, Volume 144, Page 133
[11]
Z. H. Goh, N. A. S. Mohd, S. G. Tan, S. Bhassu, and W. S. Tan
Journal of General Virology, 2014, Volume 95, Number Pt_9, Page 1919
[12]
Beatrice Sing Yieng Tou, Kian Eang Neo, Beng Ti Tey, and Michelle Yeen Tan Ng
Separation and Purification Technology, 2014, Volume 130, Page 45
[13]
Kam Yee Yoon, Wen Siang Tan, Beng Ti Tey, Khai Wooi Lee, and Kok Lian Ho
ELECTROPHORESIS, 2013, Volume 34, Number 2, Page 244
[14]
Razieh Monjezi, Sheau Wei Tan, Beng Ti Tey, Chin Chin Sieo, and Wen Siang Tan
Journal of Virological Methods, 2013, Volume 187, Number 1, Page 121
[15]
Khai Wooi Lee, Beng Ti Tey, Kok Lian Ho, Bimo A. Tejo, and Wen Siang Tan
Molecular Pharmaceutics, 2012, Volume 9, Number 9, Page 2415
[17]
Wei Boon Yap, Beng Ti Tey, Noorjahan Banu Mohamed Alitheen, and Wen Siang Tan
Journal of Chromatography A, 2010, Volume 1217, Number 21, Page 3473
[18]
K.W. Lee, B.T. Tey, K.L. Ho, and W.S. Tan
Journal of Applied Microbiology, 2012, Volume 112, Number 1, Page 119
[19]
Khai Wooi Lee and Wen Siang Tan
Journal of Virological Methods, 2008, Volume 151, Number 2, Page 172
[20]
Michelle Y.T. Ng, Wen Siang Tan, Norhafizah Abdullah, Tau Chuan Ling, and Beng Ti Tey
Journal of Virological Methods, 2006, Volume 137, Number 1, Page 134
[21]
Rattana Wongchuphan, Beng Ti Tey, Wen Siang Tan, Senthil Kumar Subramanian, Farah Saleena Taip, and Tau Chuan Ling
Process Biochemistry, 2011, Volume 46, Number 1, Page 101
[22]
Chin Woi Ho, Wen Siang Tan, Suryani Kamarudin, Tau Chuan Ling, and Beng Ti Tey
Process Biochemistry, 2008, Volume 43, Number 2, Page 206
[23]
Kyoung-Jae Choi, Chun-Woo Lim, Moon-Young Yoon, Byung-Yoon Ahn, and Yeon Gyu Yu
Biochemical and Biophysical Research Communications, 2004, Volume 319, Number 3, Page 959
[24]
Suet Lin Chia, Wen Siang Tan, Khozirah Shaari, Noorsaadah Abdul Rahman, Khatijah Yusoff, and Seetharama D. Satyanarayanajois
Peptides, 2006, Volume 27, Number 6, Page 1217
[25]
Razieh Monjezi, Beng Ti Tey, Chin Chin Sieo, and Wen Siang Tan
Journal of Chromatography B, 2010, Volume 878, Number 21, Page 1855
[26]
Michelle Y.T. Ng, Wen Siang Tan, Norhafizah Abdullah, Tau Chuan Ling, and Beng Ti Tey
Journal of Biotechnology, 2008, Volume 138, Number 3-4, Page 74
[27]
Michelle Y.T. Ng, Wen Siang Tan, Norhafizah Abdullah, Tau Chuan Ling, and Beng Ti Tey
Journal of Chromatography A, 2007, Volume 1172, Number 1, Page 47
[28]
Hyung-Kwon Lim, Thomas J. Mansell, Stephen W. Linderman, Adam C. Fisher, Michael R. Dyson, and Matthew P. DeLisa
Protein Science, 2009, Volume 18, Number 12, Page 2537
[29]
Chin Woi Ho, Wen Siang Tan, Wei Boon Yap, Tau Chuan Ling, and Beng Ti Tey
Biotechnology and Bioprocess Engineering, 2008, Volume 13, Number 5, Page 577
[30]
JC Aguilar, Y Lobaina, V Muzio, D Garcia, E Penton, E Iglesias, D Pichardo, D Urquiza, D Rodriguez, D Silva, N Petrovsky, and G Guillen
Immunology and Cell Biology, 2004, Volume 82, Number 5, Page 539

Comments (0)

Please log in or register to comment.
Log in