Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred

12 Issues per year

IMPACT FACTOR 2016: 3.273

CiteScore 2016: 3.01

SCImago Journal Rank (SJR) 2016: 1.679
Source Normalized Impact per Paper (SNIP) 2016: 0.800

See all formats and pricing
More options …
Volume 384, Issue 3 (Mar 2003)


In vitro Uptake and Stability Study of pVEC and Its All-D Analog

A. Elmquist / Ü. Langel
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/BC.2003.044


A key step in the development of new hydrophilic pharmaceuticals is to get them through biological barriers. Cell-penetrating peptides, CPPs, have been shown previously to enter cells both in vitro and in vivo by a non-endocytotic mechanism and to be able to carry large cargo molecules with them. Recently, we showed that a small peptide, pVEC, from murine vascular endothelial cadherin, has the characteristics to be classified as a protein derived CPP. Here we have further investigated pVEC together with its all-D analog for cellular uptake, intra- and extracellular stability, and their enzymatic degradation. The two peptides, pVEC and all-D pVEC, translocate into aortic endothelial cells and murine fibroblasts by a nonendocytotic mechanism. In phosphate buffer, pVEC remains intact while the C-terminal lysine is quickly removed in human serum and serumcontaining media. Both pVEC and pVEC without the Cterminal Lys were detected by mass spectrometry inside the two cell types tested. The pVEC half-life is 10.5 min in phosphate buffer containing 10 units of trypsin and 44.6 min in phosphate buffer containing 4.2 units of carboxypeptidase A and 18 units of carboxypeptidase B. In contrast to pVEC, the all-D analog remains intact in serum and resists enzymatic degradation.

About the article

Published Online: 2005-06-01

Published in Print: 2003-03-14

Citation Information: Biological Chemistry, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2003.044.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Kristina Najjar, Alfredo Erazo-Oliveras, John W. Mosior, Megan J. Whitlock, Ikram Rostane, Joseph M. Cinclair, and Jean-Philippe Pellois
Bioconjugate Chemistry, 2017
Shunsuke Yamaguchi, Shingo Ito, Mio Kurogi-Hirayama, and Sumio Ohtsuki
Journal of Controlled Release, 2017, Volume 262, Page 232
Anne Elfgen, Beatrix Santiago-Schübel, Lothar Gremer, Janine Kutzsche, and Dieter Willbold
European Journal of Pharmaceutical Sciences, 2017, Volume 107, Page 203
Kristina Najjar, Alfredo Erazo-Oliveras, Dakota J. Brock, Ting-Yi Wang, and Jean-Philippe Pellois
Journal of Biological Chemistry, 2017, Volume 292, Number 3, Page 847
Rainer Fischer, Karsten Köhler, Mariola Fotin-Mleczek, and Roland Brock
Journal of Biological Chemistry, 2004, Volume 279, Number 13, Page 12625
Wongsakorn Suchaoin, Arshad Mahmood, Kesinee Netsomboon, and Andreas Bernkop-Schnürch
Nanomedicine, 2017, Volume 12, Number 9, Page 963
P. V. G. M. Rathnayake, B. G. C. M. Gunathunge, P. N. Wimalasiri, D. N. Karunaratne, and R. J. K. U. Ranatunga
Journal of Biophysics, 2017, Volume 2017, Page 1
Sofie Stalmans, Nathalie Bracke, Evelien Wynendaele, Bert Gevaert, Kathelijne Peremans, Christian Burvenich, Ingeborgh Polis, Bart De Spiegeleer, and Je-Min Choi
PLOS ONE, 2015, Volume 10, Number 10, Page e0139652
Hae-Duck Bae, Joohyun Lee, Xing-Hai Jin, and Kyunglim Lee
Molecular Pharmaceutics, 2016, Volume 13, Number 9, Page 3196
Sónia Troeira Henriques, Manuel Nuno Melo, and Miguel A. R. B. Castanho
Molecular Membrane Biology, 2007, Volume 24, Number 3, Page 173
Christian Barucker, Heiko J. Bittner, Philip K.-Y. Chang, Scott Cameron, Mark A. Hancock, Filip Liebsch, Shireen Hossain, Anja Harmeier, Hunter Shaw, François M. Charron, Manuel Gensler, Paul Dembny, Wei Zhuang, Dietmar Schmitz, Jürgen P. Rabe, Yong Rao, Rudi Lurz, Peter W. Hildebrand, R. Anne McKinney, and Gerhard Multhaup
Scientific Reports, 2015, Volume 5, Page 15410
Jesús Fominaya, Jerónimo Bravo, and Angelita Rebollo
Therapeutic Delivery, 2015, Volume 6, Number 10, Page 1171
Qianyu Zhang, Huile Gao, and Qin He
Molecular Pharmaceutics, 2015, Volume 12, Number 9, Page 3105
Gaiqing Xue, Zhen Liu, Lingxuan Wang, and Lily Zu
Journal of Mass Spectrometry, 2015, Volume 50, Number 1, Page 220
M. Kristensen, C. Foged, J. Berthelsen, and H. Mørck Nielsen
Journal of Drug Delivery Science and Technology, 2013, Volume 23, Number 4, Page 365
Tohru Yamada, Sara Signorelli, Salvatore Cannistraro, Craig W. Beattie, and Anna Rita Bizzarri
Molecular Pharmaceutics, 2015, Volume 12, Number 1, Page 140
Marco Emanuele Favretto and Roland Brock
Small, 2015, Volume 11, Number 12, Page 1414
Sai Ma, Betsy Schroeder, Chen Sun, Despina Nelie Loufakis, Zhenning Cao, Nammalwar Sriranganathan, and Chang Lu
Integr. Biol., 2014, Volume 6, Number 10, Page 973
Fe-Lin Lin Wu, Tzyy-Harn Yeh, Ying-Luen Chen, Yu-Chin Chiu, Ju-Chen Cheng, Ming-Feng Wei, and Li-Jiuan Shen
Molecular Pharmaceutics, 2014, Volume 11, Number 8, Page 2777
Veerle Kersemans and Bart Cornelissen
Pharmaceuticals, 2010, Volume 3, Number 3, Page 600
Anjali Apte, Erez Koren, Alexander Koshkaryev, and Vladimir P Torchilin
Cancer Biology & Therapy, 2014, Volume 15, Number 1, Page 69
G. Carmona, A. Rodriguez, D. Juarez, G. Corzo, and E. Villegas
The Protein Journal, 2013, Volume 32, Number 6, Page 456
Nirupam Purkayastha, Klaus Eyer, Tom Robinson, Petra S. Dittrich, Albert K. Beck, Dieter Seebach, Beata Kolesinska, and Riccardo Cadalbert
Chemistry & Biodiversity, 2013, Volume 10, Number 7, Page 1165
Chérine Bechara and Sandrine Sagan
FEBS Letters, 2013, Volume 587, Number 12, Page 1693
Külliki Saar, Maria Lindgren, Mats Hansen, Emelía Eiríksdóttir, Yang Jiang, Katri Rosenthal-Aizman, Meeri Sassian, and Ülo Langel
Analytical Biochemistry, 2005, Volume 345, Number 1, Page 55
Silvia Cavalli, Daniel Carbajo, Milena Acosta, Silvia Lope-Piedrafita, Ana Paula Candiota, Carles Arús, Miriam Royo, and Fernando Albericio
Chemical Communications, 2012, Volume 48, Number 43, Page 5322
Tina Holm, Semharai Netzereab, Mats Hansen, Ülo Langel, and Mattias Hällbrink
FEBS Letters, 2005, Volume 579, Number 23, Page 5217
El-Sayed Khafagy and Mariko Morishita
Advanced Drug Delivery Reviews, 2012, Volume 64, Number 6, Page 531
Archana Chugh and François Eudes
FEBS Journal, 2008, Volume 275, Number 10, Page 2403
Jae Sam Lee and Ching-Hsuan Tung
Biopolymers, 2011, Volume 96, Number 6, Page 772
Yong Hai Nan, Il-Seon Park, Kyung-Soo Hahm, and Song Yub Shin
Journal of Peptide Science, 2011, Volume 17, Number 12, Page 812
Wouter P.R. Verdurmen, Petra H. Bovee-Geurts, Parvesh Wadhwani, Anne S. Ulrich, Mattias Hällbrink, Toin H. van Kuppevelt, and Roland Brock
Chemistry & Biology, 2011, Volume 18, Number 8, Page 1000
Tina Holm, Helin Räägel, Samir EL Andaloussi, Margot Hein, Maarja Mäe, Margus Pooga, and Ülo Langel
Biochimica et Biophysica Acta (BBA) - Biomembranes, 2011, Volume 1808, Number 6, Page 1544
Arwyn T. Jones
International Journal of Pharmaceutics, 2008, Volume 354, Number 1-2, Page 34
Rachel Tréhin and Hans P Merkle
European Journal of Pharmaceutics and Biopharmaceutics, 2004, Volume 58, Number 2, Page 209
Kalle Kilk, Riina Mahlapuu, Ursel Soomets, and Ülo Langel
Toxicology, 2009, Volume 265, Number 3, Page 87
Gunnar P.H. Dietz and Mathias Bähr
Molecular and Cellular Neuroscience, 2004, Volume 27, Number 2, Page 85
Erez Koren, Anjali Apte, Rupa R. Sawant, Jacob Grunwald, and Vladimir P. Torchilin
Drug Delivery, 2011, Volume 18, Number 5, Page 377
Michael E. Herbig, Ursina Fromm, Jeannine Leuenberger, Ulrike Krauss, Annette G. Beck-Sickinger, and Hans P. Merkle
Biochimica et Biophysica Acta (BBA) - Biomembranes, 2005, Volume 1712, Number 2, Page 197
Anna Elmquist, Mats Hansen, and Ülo Langel
Biochimica et Biophysica Acta (BBA) - Biomembranes, 2006, Volume 1758, Number 6, Page 721
Maarja Mäe, Helena Myrberg, Yang Jiang, Heiti Paves, Andres Valkna, and Ülo Langel
Biochimica et Biophysica Acta (BBA) - Biomembranes, 2005, Volume 1669, Number 2, Page 101
Robert Rennert, Christian Wespe, Annette G. Beck-Sickinger, and Ines Neundorf
Biochimica et Biophysica Acta (BBA) - Biomembranes, 2006, Volume 1758, Number 3, Page 347
Sílvia Pujals, Jimena Fernández-Carneado, Carmen López-Iglesias, Marcelo J. Kogan, and Ernest Giralt
Biochimica et Biophysica Acta (BBA) - Biomembranes, 2006, Volume 1758, Number 3, Page 264
Soline Aubry, Baptiste Aussedat, Diane Delaroche, Chen-Yu Jiao, Gérard Bolbach, Solange Lavielle, Gérard Chassaing, Sandrine Sagan, and Fabienne Burlina
Biochimica et Biophysica Acta (BBA) - Biomembranes, 2010, Volume 1798, Number 12, Page 2182
Noriyasu Kamei, Mariko Morishita, Jumpei Ehara, and Kozo Takayama
Journal of Controlled Release, 2008, Volume 131, Number 2, Page 94
Noriyasu Kamei, Mariko Morishita, Yoshimi Eda, Nobuo Ida, Reiji Nishio, and Kozo Takayama
Journal of Controlled Release, 2008, Volume 132, Number 1, Page 21
El-Sayed Khafagy, Mariko Morishita, Koichi Isowa, Jun Imai, and Kozo Takayama
Journal of Controlled Release, 2009, Volume 133, Number 2, Page 103
Robert Rennert, Ines Neundorf, and Annette G. Beck-Sickinger
Advanced Drug Delivery Reviews, 2008, Volume 60, Number 4-5, Page 485
Sílvia Pujals and Ernest Giralt
Advanced Drug Delivery Reviews, 2008, Volume 60, Number 4-5, Page 473
Mats Hansen, Kalle Kilk, and Ülo Langel
Advanced Drug Delivery Reviews, 2008, Volume 60, Number 4-5, Page 572
Archana Chugh, François Eudes, and Youn-Seb Shim
IUBMB Life, 2010, Volume 62, Number 3, Page 183
Yibing Huang, Jinfeng Huang, and Yuxin Chen
Protein & Cell, 2010, Volume 1, Number 2, Page 143
Archana Chugh, Eric Amundsen, and François Eudes
Plant Cell Reports, 2009, Volume 28, Number 5, Page 801
Camilla Foged and Hanne Moerck Nielsen
Expert Opinion on Drug Delivery, 2008, Volume 5, Number 1, Page 105
Christina Foerg, Kathrin M. Weller, Helene Rechsteiner, Hanne M. Nielsen, Jimena Fernández-Carneado, René Brunisholz, Ernest Giralt, and Hans P. Merkle
The AAPS Journal, 2008, Volume 10, Number 2, Page 349
Archana Chugh and François Eudes
Journal of Peptide Science, 2008, Volume 14, Number 4, Page 477
Sílvia Pujals, Jimena Fernández-Carneado, M. Dolors Ludevid, and Ernest Giralt
ChemMedChem, 2008, Volume 3, Number 2, Page 296
Christina Foerg and Hans P. Merkle
Journal of Pharmaceutical Sciences, 2008, Volume 97, Number 1, Page 144
Leena N. Patel, Jennica L. Zaro, and Wei-Chiang Shen
Pharmaceutical Research, 2007, Volume 24, Number 11, Page 1977
Yuxin Chen, Adriana I. Vasil, Linda Rehaume, Colin T. Mant, Jane L. Burns, Michael L. Vasil, Robert E. W. Hancock, and Robert S. Hodges
Chemical Biology Drug Design, 2006, Volume 67, Number 2, Page 162
Ferenc Hudecz, Zoltán Bánóczi, and Gabriella Csík
Medicinal Research Reviews, 2005, Volume 25, Number 6, Page 679
Fabienne Burlina, Sandrine Sagan, Gérard Bolbach, and Gérard Chassaing
Angewandte Chemie, 2005, Volume 117, Number 27, Page 4316
Fabienne Burlina, Sandrine Sagan, Gérard Bolbach, and Gérard Chassaing
Angewandte Chemie International Edition, 2005, Volume 44, Number 27, Page 4244

Comments (0)

Please log in or register to comment.
Log in