Jump to ContentJump to Main Navigation
Show Summary Details
In This Section

Thomas, Douglas D.

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Turk, Boris / Wittinghofer, Alfred

12 Issues per year


IMPACT FACTOR 2016: 3.273

CiteScore 2016: 3.01

SCImago Journal Rank (SJR) 2015: 1.607
Source Normalized Impact per Paper (SNIP) 2015: 0.751

Online
ISSN
1437-4315
See all formats and pricing
In This Section
Volume 384, Issue 3 (Mar 2003)

Issues

A Putative Glutathione Peroxidase of Drosophila Encodes a Thioredoxin Peroxidase That Provides Resistance against Oxidative Stress But Fails to Complement a Lack of Catalase Activity

F. Missirlis / S. Rahlfs / N. Dimopoulos / H. Bauer / K. Becker / A. Hilliker / J.P. Phillips / H. Jäckle
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/BC.2003.052

Abstract

Cellular defense systems against reactive oxygen species (ROS) include thioredoxin reductase (TrxR) and glutathione reductase (GR). They generate sulfhydryl-reducing systems which are coupled to antioxidant enzymes, the thioredoxin and glutathione peroxidases (TPx and GPx). The fruit fly Drosophila lacks a functional GR, suggesting that the thioredoxin system is the major source for recycling glutathione. Whole genome in silico analysis identified two non-selenium containing putative GPx genes. We examined the biochemical characteristics of one of these gene products and found that it lacks GPx activity and functions as a TPx. Transgene-dependent overexpression of the newly identified Glutathione peroxidase homolog with thioredoxin peroxidase activity (Gtpx-1) gene increases resistance to experimentally induced oxidative stress, but does not compensate for the loss of catalase, an enzyme which, like GTPx-1, functions to eliminate hydrogen peroxide. The results suggest that GTPx-1 is part of the Drosophila Trx antioxidant defense system but acts in a genetically distinct pathway or in a different cellular compartment than catalase.

About the article

Published Online: 2005-06-01

Published in Print: 2003-03-14



Citation Information: Biological Chemistry, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2003.052. Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Madia Trujillo, Beatriz Alvarez, and Rafael Radi
Free Radical Research, 2015, Page 1
[2]
Emilio Pimentel, Luz M. Vidal, Martha Patricia Cruces, and Mariusz Krzysztof Janczur
Open Journal of Animal Sciences, 2013, Volume 03, Number 04, Page 1
[3]
Chengcheng Li, Baohua Xu, Yuxi Wang, Zaibin Yang, and Weiren Yang
Entomologia Experimentalis et Applicata, 2014, Volume 151, Number 1, Page 19
[4]
Mitsuko Hirosawa-Takamori, Ho-Ryun Chung, and Herbert Jäckle
EMBO reports, 2004, Volume 5, Number 3, Page 317
[5]
F. Missirlis, S. Holmberg, T. Georgieva, B. C. Dunkov, T. A. Rouault, and J. H. Law
Proceedings of the National Academy of Sciences, 2006, Volume 103, Number 15, Page 5893
[6]
William C. Orr, Svetlana N. Radyuk, and Rajindar S. Sohal
Antioxidants & Redox Signaling, 2013, Volume 19, Number 8, Page 788
[7]
Pengbo Yao, Wenjing Lu, Fei Meng, Xiuling Wang, Baohua Xu, and Xingqi Guo
Journal of Insect Physiology, 2013, Volume 59, Number 3, Page 273
[8]
Matilde Maiorino, Fulvio Ursini, Valentina Bosello, Stefano Toppo, Silvio C.E. Tosatto, Pierluigi Mauri, Katja Becker, Antonella Roveri, Cristiana Bulato, Louise Benazzi, Antonella De Palma, and Leopold Flohé
Journal of Molecular Biology, 2007, Volume 365, Number 4, Page 1033
[9]
Sergey A Sinenko, Jiwon Shim, and Utpal Banerjee
EMBO reports, 2011, Volume 13, Number 1, Page 83
[11]
Stylianos Kosmidis, Jose A. Botella, Konstantinos Mandilaras, Stephan Schneuwly, Efthimios M.C. Skoulakis, Tracey A. Rouault, and Fanis Missirlis
Neurobiology of Disease, 2011, Volume 43, Number 1, Page 213
[12]
Li-Hong Yang, Hai Huang, and Jin-Jun Wang
Journal of Insect Physiology, 2010, Volume 56, Number 12, Page 1871
[13]
M.J. Lehane, W. Gibson, and S.M. Lehane
International Journal for Parasitology, 2008, Volume 38, Number 1, Page 93
[14]
Stefano Toppo, Stefano Vanin, Valentina Bosello, and Silvio C.E. Tosatto
Antioxidants & Redox Signaling, 2008, Volume 10, Number 9, Page 1501
[15]
Edward Owusu-Ansah and Utpal Banerjee
Nature, 2009, Volume 461, Number 7263, Page 537
[16]
L. Flohé, T. Jaeger, S. Pilawa, and H. Sztajer
Redox Report, 2003, Volume 8, Number 5, Page 256
[17]
Dora Dias-Santagata, Tudor A. Fulga, Atanu Duttaroy, and Mel B. Feany
Journal of Clinical Investigation, 2007, Volume 117, Number 1, Page 236
[18]
M. Corona and G. E. Robinson
Insect Molecular Biology, 2006, Volume 15, Number 5, Page 687

Comments (0)

Please log in or register to comment.
Log in