Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred

IMPACT FACTOR 2018: 3.014
5-year IMPACT FACTOR: 3.162

CiteScore 2018: 3.09

SCImago Journal Rank (SJR) 2018: 1.482
Source Normalized Impact per Paper (SNIP) 2018: 0.820

See all formats and pricing
More options …
Volume 384, Issue 3


Characterization of Calmodulin Binding to the Orphan Nuclear Receptor ERRγ

M. Hentschke / C. Schulze / U. Süsens / U. Borgmeyer
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/BC.2003.053


The estrogen receptorrelated receptor γ (ERγ/ERR3/NR3B3), a member of the nuclear receptor superfamily, activates transcription in the absence of ligands. In order to identify ligand-independent mechanisms of activation, we tested whether calmodulin (CaM), a key regulator of numerous cellular processes and a predominant intracellular receptor for Ca2+-signals, interacts with ERRγ. In vitro pulldown experiments with calmodulin-Sepharose demonstrated a Ca2+-dependent interaction with cellularly expressed ERRγ. As shown by truncation analysis, the CaM binding site is highly unusual in that it is composed of two discontinuous elements. Moreover, by surface plasmon resonance (SPR) biosensor technology, we detected a direct interaction of immobilized bacterially expressed ERRγ fusion protein with Ca2+-calmodulin. This is best described by a model which assumes a conformational change of the initially formed complex to a more stable form. Whereas in vitro DNA binding was calmodulinindependent, transient transfection analysis revealed a Ca2+-influxdependent ERRγmediated transcriptional activation of a luciferase reporter gene. Thus, we propose that CaM acts as a mediator in the Ca2+-dependent modulation of ERRγ.

About the article

Published Online: 2005-06-01

Published in Print: 2003-03-14

Citation Information: Biological Chemistry, Volume 384, Issue 3, Pages 473–482, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2003.053.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Mi Ae Kim and Young Chang Sohn
Zoological Science, 2017, Volume 34, Number 3, Page 235
Monika Puzianowska-Kuznicka, Eliza Pawlik-Pachucka, Magdalena Owczarz, Monika Budzińska, and Jacek Polosak
International Journal of Endocrinology, 2013, Volume 2013, Page 1
Eiji Fujii, Kaori Nakamura, Shin‐ichi Sasaki, Daniel Citterio, Kazuyoshi Kurihara, and Koji Suzuki
Instrumentation Science & Technology, 2003, Volume 31, Number 4, Page 343
Moritz Hentschke and Uwe Borgmeyer
Biochemical and Biophysical Research Communications, 2003, Volume 312, Number 4, Page 975
Dominique Gallo, Yves Jacquot, Guy Laurent, and Guy Leclercq
Molecular and Cellular Endocrinology, 2008, Volume 291, Number 1-2, Page 20
Ki Lui, Yu Huang, H.L. Choi, Shan Yu, K.B. Wong, Shiuan Chen, and Franky L. Chan
The Prostate, 2006, Volume 66, Number 15, Page 1600
Rebecca L. Rich and David G. Myszka
Journal of Molecular Recognition, 2005, Volume 18, Number 1, Page 1

Comments (0)

Please log in or register to comment.
Log in