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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred

12 Issues per year

IMPACT FACTOR 2017: 3.022

CiteScore 2017: 2.81

SCImago Journal Rank (SJR) 2017: 1.562
Source Normalized Impact per Paper (SNIP) 2017: 0.705

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Volume 384, Issue 6


T47-D Cells and Type V Collagen: A Model for the Study of Apoptotic Gene Expression by Breast Cancer Cells

C. Luparello / F. David / G. Campisi / R. Sirchia
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/BC.2003.109


We have previously reported that type V collagen is a poorly adhesive, anti-proliferative and motility-inhibitory substrate for the 8701-BC breast cancer cell line, which also triggers DNA fragmentation and impairs survival of the same cell line. In the present work we have extended to other breast cancer cell lines (T47-D, MDA-MB231, Hs578T) our investigation of type V collagen influence on the DNA status and cell survival, also examining whether adhesion and growth of cells on this collagen substrate could exert some effect on the expression level of selected apoptosis related genes. We report here that, among the cell lines tested, only T47-D is responsive to the deathpromoting influence of type V collagen. In addition, the latter induces changes in gene expression by upregulating p53, Waf-1, Cas, Dap kinase and caspases 1, -5 and -14 and downregulating Bcl-2. Our data validate the T47-D line as a suitable in vitro model for further and more detailed studies on the molecular mechanisms of the death response induced by type V collagen on mammary tumor cells.

About the article

Published Online: 2005-06-01

Published in Print: 2003-06-16

Citation Information: Biological Chemistry, Volume 384, Issue 6, Pages 965–975, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2003.109.

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