Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred

12 Issues per year

IMPACT FACTOR 2017: 3.022

CiteScore 2017: 2.81

SCImago Journal Rank (SJR) 2017: 1.562
Source Normalized Impact per Paper (SNIP) 2017: 0.705

See all formats and pricing
More options …
Volume 385, Issue 10


Inter- and intra-molecular distances determined by EPR spectroscopy and site-directed spin labeling reveal protein-protein and protein-oligonucleotide interaction

Heinz-Jürgen Steinhoff
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/BC.2004.119


Recent developments including pulse and multi-frequency techniques make the combination of site-directed spin labeling and electron paramagnetic resonance (EPR) spectroscopy an attractive approach for the study of protein-protein or protein-oligonucleotide interaction. Analysis of the spin label side chain mobility, its solvent accessibility, the polarity of the spin label micro-environment and distances between spin label side chains allow the modeling of protein domains or protein-protein interaction sites and their conformational changes with a spatial resolution at the level of the backbone fold. Structural changes can be detected with millisecond time resolution. Inter- and intra-molecular distances are accessible in the range from approximately 0.5 to 8 nm by the combination of continuous wave and pulse EPR methods. Recent applications include the study of transmembrane substrate transport, membrane channel gating, gene regulation and signal transfer.

Keywords: bacteriorhodopsin; inter-spin distance; proline permease; pulse EPR; sensory rhodopsin

About the article

Published Online: 2005-06-01

Published in Print: 2004-10-01

Citation Information: Biological Chemistry, Volume 385, Issue 10, Pages 913–920, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2004.119.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Jacob Blaffert, Haleh Hashemi Haeri, Michaela Blech, Dariush Hinderberger, and Patrick Garidel
Analytical Biochemistry, 2018
Nilesh R. Kamble and Snorri Th. Sigurdsson
Chemistry - A European Journal, 2018
Indra D. Sahu and Gary A. Lorigan
BioMed Research International, 2018, Volume 2018, Page 1
Raquel Fonseca-Maldonado, Luana P. Meleiro, Luís F. S. Mendes, Luana F. Alves, Sibeli Carli, Lucas D. Morero, Luis G. M. Basso, Antonio J. Costa-Filho, and Richard J. Ward
Biotechnology for Biofuels, 2017, Volume 10, Number 1
N. Voskoboynikova, W. Mosslehy, A. Colbasevici, T. T. Ismagulova, D. V. Bagrov, A. A. Akovantseva, P. S. Timashev, A. Y. Mulkidjanian, V. N. Bagratashvili, K. V. Shaitan, M. P. Kirpichnikov, and H.-J. Steinhoff
RSC Advances, 2017, Volume 7, Number 81, Page 51324
Darío Ortiz de Orué Lucana, Mareike Roscher, Alf Honigmann, and Julia Schwarz
Journal of Biological Chemistry, 2010, Volume 285, Number 36, Page 28086
Sabine Böhme, Simon Meyer, André Krüger, Heinz-Jürgen Steinhoff, Alfred Wittinghofer, and Johann P. Klare
Journal of Biological Chemistry, 2010, Volume 285, Number 22, Page 16991
Qi Cai, Ana Karin Kusnetzow, Wayne L. Hubbell, Ian S. Haworth, Gian Paola C. Gacho, Ned Van Eps, Kálmán Hideg, Eric J. Chambers, and Peter Z. Qin
Nucleic Acids Research, 2006, Volume 34, Number 17, Page 4722
Daniel Klose, Johann P. Klare, Dina Grohmann, Christopher W. M. Kay, Finn Werner, Heinz-Jürgen Steinhoff, and Bin Xue
PLoS ONE, 2012, Volume 7, Number 6, Page e39492
Jessica A. Clayton, Mian Qi, Adelheid Godt, Daniella Goldfarb, Songi Han, and Mark S. Sherwin
Phys. Chem. Chem. Phys., 2017, Volume 19, Number 7, Page 5127
Simon Meyer, Sabine Böhme, André Krüger, Heinz-Jürgen Steinhoff, Johann P. Klare, Alfred Wittinghofer, and Gregory A. Petsko
PLoS Biology, 2009, Volume 7, Number 10, Page e1000212
Adam N. Smith, Umar T. Twahir, Thierry Dubroca, Gail E. Fanucci, and Joanna R. Long
The Journal of Physical Chemistry B, 2016, Volume 120, Number 32, Page 7880
Dimitri Bytchenkoff and Stéphane Rodts
Journal of Magnetic Resonance, 2015, Volume 261, Page 54
Daniel Nohr, Ryan Rodriguez, Stefan Weber, and Erik Schleicher
Frontiers in Molecular Biosciences, 2015, Volume 2
Felix G M Ernst, Christian Rickert, Alexander Bluschke, Heike Betat, Heinz-Jürgen Steinhoff, and Mario Mörl
RNA Biology, 2015, Volume 12, Number 4, Page 435
Thomas Pohl, Thomas Spatzal, Müge Aksoyoglu, Erik Schleicher, Arpad Mihai Rostas, Helga Lay, Udo Glessner, Corinne Boudon, Petra Hellwig, Stefan Weber, and Thorsten Friedrich
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2010, Volume 1797, Number 12, Page 1894
Dnyaneshwar B. Gophane, Burkhard Endeward, Thomas F. Prisner, and Snorri Th. Sigurdsson
Chemistry - A European Journal, 2014, Volume 20, Number 48, Page 15913
Christoph Göbl, Tobias Madl, Bernd Simon, and Michael Sattler
Progress in Nuclear Magnetic Resonance Spectroscopy, 2014, Volume 80, Page 26
Derek Marsh
Journal of Magnetic Resonance, 2014, Volume 238, Page 77
Ezgi Karaca and Alexandre M. J. J. Bonvin
Acta Crystallographica Section D Biological Crystallography, 2013, Volume 69, Number 5, Page 683
Inga Hänelt, Dorith Wunnicke, Enrica Bordignon, Heinz-Jürgen Steinhoff, and Dirk Jan Slotboom
Nature Structural & Molecular Biology, 2013, Volume 20, Number 2, Page 210
Ezgi Karaca and Alexandre M.J.J. Bonvin
Methods, 2013, Volume 59, Number 3, Page 372
G. W. Reginsson, S. A. Shelke, C. Rouillon, M. F. White, S. T. Sigurdsson, and O. Schiemann
Nucleic Acids Research, 2013, Volume 41, Number 1, Page e11
Yasar Akdogan, Matthias J. N. Junk, and Dariush Hinderberger
Biomacromolecules, 2011, Volume 12, Number 4, Page 1072
Yasar Akdogan, Veerappan Anbazhagan, Dariush Hinderberger, and Dirk Schneider
Biochemistry, 2012, Volume 51, Number 36, Page 7149
Simone Popp, Lars Packschies, Nicole Radzwill, Klaus Peter Vogel, Heinz-Jürgen Steinhoff, and Jochen Reinstein
Journal of Molecular Biology, 2005, Volume 347, Number 5, Page 1039
Mikhail Y. Berezin, Hyeran Lee, Walter Akers, and Samuel Achilefu
Biophysical Journal, 2007, Volume 93, Number 8, Page 2892
Yasar Akdogan and Dariush Hinderberger
The Journal of Physical Chemistry B, 2011, Volume 115, Number 51, Page 15422
Silvia Carlotto, Mirco Zerbetto, Maryam Hashemi Shabestari, Alessandro Moretto, Fernando Formaggio, Marco Crisma, Claudio Toniolo, Martina Huber, and Antonino Polimeno
The Journal of Physical Chemistry B, 2011, Volume 115, Number 44, Page 13026
Xu. Wang, Hsiau-Wei Lee, Yizhou Liu, and James H. Prestegard
Journal of Structural Biology, 2011, Volume 173, Number 3, Page 515
Silvia Domingo Köhler, Martin Spitzbarth, Kay Diederichs, Thomas E. Exner, and Malte Drescher
Journal of Magnetic Resonance, 2011, Volume 208, Number 1, Page 167
Marzena Jankowska-Anyszka, Karolina Piecyk, and Jelena Šamonina-Kosicka
Organic & Biomolecular Chemistry, 2011, Volume 9, Number 15, Page 5564
Aleksander Czogalla, Krzysztof Grzymajło, Adam Jezierski, and Aleksander F. Sikorski
Biochimica et Biophysica Acta (BBA) - Biomembranes, 2008, Volume 1778, Number 11, Page 2612
David D. Thomas, David Kast, and Vicci L. Korman
Annual Review of Biophysics, 2009, Volume 38, Number 1, Page 347
Ute A. Hellmich and Clemens Glaubitz
Biological Chemistry, 2009, Volume 390, Number 8
Christian Beier and Martin Zacharias
Expert Opinion on Drug Discovery, 2010, Volume 5, Number 4, Page 347
Karen Wright, Michel Wakselman, Jean-Paul Mazaleyrat, Lorenzo Franco, Antonio Toffoletti, Fernando Formaggio, and Claudio Toniolo
Chemistry - A European Journal, 2010, Volume 16, Number 36, Page 11160
Nivrutti Barhate, Pavol Cekan, Archna P. Massey, and Snorri Th. Sigurdsson
Angewandte Chemie, 2007, Volume 119, Number 15, Page 2709
Yasar Akdogan, Jeannine Heller, Herbert Zimmermann, and Dariush Hinderberger
Physical Chemistry Chemical Physics, 2010, Volume 12, Number 28, Page 7874
Yi Xue, Ivan S. Podkorytov, D. Krishna Rao, Nathan Benjamin, Honglei Sun, and Nikolai R. Skrynnikov
Protein Science, 2009, Volume 18, Number 7, Page 1401
Pétur O. Heidarsson, Snorri Th Sigurdsson, and Bjarni Ásgeirsson
FEBS Journal, 2009, Volume 276, Number 10, Page 2725
Olav Schiemann, Nelly Piton, Jörn Plackmeyer, Bela E Bode, Thomas F Prisner, and Joachim W Engels
Nature Protocols, 2007, Volume 2, Number 4, Page 904
Peter Z Qin, Ian S Haworth, Qi Cai, Ana K Kusnetzow, Gian Paola G Grant, Eric A Price, Glenna Z Sowa, Anna Popova, Bruno Herreros, and Honghang He
Nature Protocols, 2007, Volume 2, Number 10, Page 2354
Pavol Cekan, Alyssa L. Smith, Nivrutti Barhate, Bruce H. Robinson, and Snorri Th. Sigurdsson
Nucleic Acids Research, 2008, Volume 36, Number 18, Page 5946
Olav Schiemann and Thomas F. Prisner
Quarterly Reviews of Biophysics, 2007, Volume 40, Number 01, Page 1
H. Jung, T. Pirch, and D. Hilger
Journal of Membrane Biology, 2006, Volume 213, Number 2, Page 119
Nivrutti Barhate, Pavol Cekan, Archna P. Massey, and Snorri Th. Sigurdsson
Angewandte Chemie International Edition, 2007, Volume 46, Number 15, Page 2655
Christian F. W. Becker, Kester Lausecker, Mária Balog, Tamás Kálai, Kálmán Hideg, Heinz-Jürgen Steinhoff, and Martin Engelhard
Magnetic Resonance in Chemistry, 2005, Volume 43, Number S1, Page S34

Comments (0)

Please log in or register to comment.
Log in