Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred


IMPACT FACTOR 2018: 3.014
5-year IMPACT FACTOR: 3.162

CiteScore 2018: 3.09

SCImago Journal Rank (SJR) 2018: 1.482
Source Normalized Impact per Paper (SNIP) 2018: 0.820

Online
ISSN
1437-4315
See all formats and pricing
More options …
Volume 385, Issue 9

Issues

RNA interference by small hairpin RNAs synthesised under control of the human 7S K RNA promoter

Dorota Koper-Emde
  • Lehrstuhl für Biochemie I, Fakultät für Chemie, Ruhr-Universität Bochum, D-44780 Bochum, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Lutz Herrmann
  • Lehrstuhl für Biochemie I, Fakultät für Chemie, Ruhr-Universität Bochum, D-44780 Bochum, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Björn Sandrock
  • Lehrstuhl für Biochemie I, Fakultät für Chemie, Ruhr-Universität Bochum, D-44780 Bochum, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Bernd-Joachim Benecke
  • Lehrstuhl für Biochemie I, Fakultät für Chemie, Ruhr-Universität Bochum, D-44780 Bochum, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/BC.2004.103

Abstract

Small interfering RNAs (siRNAs) represent RNA duplexes of 21 nucleotides in length that inhibit gene expression. We have used the human gene-external 7S K RNA promoter for synthesis of short hairpin RNAs (shRNAs) which efficiently target human lamin mRNA via RNA interference (RNAi). Here we demonstrate that orientation of the target sequence within the shRNA construct is important for interference. Furthermore, effective interference also depends on the length and/or structure of the shRNA. Evidence is presented that the human 7S K promoter is more active in vivo than other gene-external promoters, such as the human U6 small nuclear RNA (snRNA) gene promoter.

Keywords: gene silencing; lamin A/C; pol III transcription; siRNA

About the article

Received: May 26, 2004

Accepted: July 16, 2004

Published Online: 2005-06-01

Published in Print: 2004-09-01


Citation Information: Biological Chemistry, Volume 385, Issue 9, Pages 791–794, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2004.103.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Stefanie A Knoepfel
World Journal of Virology, 2012, Volume 1, Number 3, Page 79
[2]
Guang-Dong Pan, Jian-Qing Yang, Lv-Nan Yan, Guang-Ping Chu, Qiang Liu, Yi Xiao, and Lin Yuan
World Journal of Gastroenterology, 2009, Volume 15, Number 4, Page 431
[4]
Gene-Errol E. Ringpis, Saki Shimizu, Hubert Arokium, Joanna Camba-Colón, Maria V. Carroll, Ruth Cortado, Yiming Xie, Patrick Y. Kim, Anna Sahakyan, Emily L. Lowe, Munetoshi Narukawa, Fadi N. Kandarian, Bryan P. Burke, Geoff P. Symonds, Dong Sung An, Irvin S. Y. Chen, Masakazu Kamata, and Roberto F. Speck
PLoS ONE, 2012, Volume 7, Number 12, Page e53492
[5]
Sara L. Montgomery, Wade C. Narrow, Michael A. Mastrangelo, John A. Olschowka, M. Kerry O'Banion, and William J. Bowers
The American Journal of Pathology, 2013, Volume 182, Number 6, Page 2285
[6]
Vihra N. Sotirova, Margaret A. Calciano, Winfried Krueger, and Marc Lalande
Plasmid, 2006, Volume 55, Number 3, Page 216
[7]
Stepan P. Chumakov, Julia E. Kravchenko, Vladimir S. Prassolov, Elena I. Frolova, and Peter M. Chumakov
Plasmid, 2010, Volume 63, Number 3, Page 143
[8]
Chin-kai Chuang, Kun-Hsiung Lee, Chiu-Tin Fan, and Yu-Show Su
Animal Biotechnology, 2009, Volume 20, Number 1, Page 34
[9]
Seow Theng Ong, Feng Li, Juan Du, Yu Wen Tan, and Shu Wang
Human Gene Therapy, 2005, Volume 16, Number 12, Page 1404
[10]
Xue-Ming Xu, Min-Hyuk Yoo, Bradley A Carlson, Vadim N Gladyshev, and Dolph L Hatfield
Nature Protocols, 2009, Volume 4, Number 9, Page 1338
[11]
Jianguo Su, Zuoyan Zhu, Feng Xiong, and Yaping Wang
Marine Biotechnology, 2008, Volume 10, Number 5, Page 511
[12]
Olivier ter Brake, Karen 't Hooft, Ying Poi Liu, Mireille Centlivre, Karin Jasmijn von Eije, and Ben Berkhout
Molecular Therapy, 2008, Volume 16, Number 3, Page 557
[13]
L. S. Lambeth, T. G. Wise, R. J. Moore, M. S. Muralitharan, and T. J. Doran
Animal Genetics, 2006, Volume 37, Number 4, Page 369
[14]
Petri I. Mäkinen, Jonna K. Koponen, Anna-Mari Kärkkäinen, Tarja M. Malm, Kati H. Pulkkinen, Jari Koistinaho, Mikko P. Turunen, and Seppo Ylä-Herttuala
The Journal of Gene Medicine, 2006, Volume 8, Number 4, Page 433
[15]
Seow Theng Ong, Feng Li, Juan Du, Yu Wen Tan, and Shu Wang
Human Gene Therapy, 2005, Volume 0, Number 0, Page 051114062814001

Comments (0)

Please log in or register to comment.
Log in