Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred

IMPACT FACTOR 2018: 3.014
5-year IMPACT FACTOR: 3.162

CiteScore 2018: 3.09

SCImago Journal Rank (SJR) 2018: 1.482
Source Normalized Impact per Paper (SNIP) 2018: 0.820

See all formats and pricing
More options …
Volume 386, Issue 10


New glycyl radical enzymes catalysing key metabolic steps in anaerobic bacteria

Thorsten Selmer / Antonio J. Pierik / Johann Heider
Published Online: 2005-10-12 | DOI: https://doi.org/10.1515/BC.2005.114


During the last decade, an increasing number of new enzymes containing glycyl radicals in their active sites have been identified and biochemically characterised. These include benzylsuccinate synthase (Bss), 4-hydroxyphenylacetate decarboxylase (Hpd) and the coenzyme B12-independent glycerol dehydratase (Gdh). These are involved in metabolic pathways as different as anaerobic toluene metabolism, fermentative production of p-cresol and glycerol fermentation. Some features of these newly discovered enzymes are described and compared with those of the previously known glycyl radical enzymes pyruvate formate-lyase (Pfl) and anaerobic ribonucleotide reductase (Nrd). Among the new enzymes, Bss and Hpd share the presence of small subunits, the function of which in the catalytic mechanisms is still enigmatic, and both enzymes contain metal centres in addition to the glycyl radical prosthetic group. The activating enzymes of the novel systems also deviate from the standard type, containing at least one additional Fe-S cluster. Finally, the available whole-genome sequences of an increasing number of strictly or facultative anaerobic bacteria revealed the presence of many more hitherto unknown glycyl radical enzyme (GRE) systems. Recent studies suggest that the particular types of these enzymes represent the ends of different evolutionary lines, which emerged early in evolution and diversified to yield remarkably versatile biocatalysts for chemical reactions that are otherwise difficult to perform in anoxic environments.

Keywords: anaerobic ribonucleotide reductase; benzylsuccinate synthase; glycerol dehydratase; 4-hydroxyphenylacetate decarboxylase; pyruvate formate-lyase

About the article

Corresponding author

Published Online: 2005-10-12

Published in Print: 2005-10-01

Citation Information: Biological Chemistry, Volume 386, Issue 10, Pages 981–988, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2005.114.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Wen-Bing Jin, Sheng Wu, Yi-Fan Xu, Hua Yuan, and Gong-Li Tang
Natural Product Reports, 2019
Karen Rossmassler, Christopher D. Snow, Dora Taggart, Casey Brown, and Susan K. De Long
Applied Microbiology and Biotechnology, 2019, Volume 103, Number 10, Page 4177
Bryan Ferlez, Markus Sutter, Cheryl A. Kerfeld, and Danielle A. Garsin
mBio, 2019, Volume 10, Number 1
Lauren J. Rajakovich and Emily P. Balskus
Natural Product Reports, 2019
Alejandro Acosta-González, Ramon Rosselló-Móra, and Silvia Marqués
Applied and Environmental Microbiology, 2013, Volume 79, Number 12, Page 3667
Ana Martínez-del Campo, Smaranda Bodea, Hilary A. Hamer, Jonathan A. Marks, Henry J. Haiser, Peter J. Turnbaugh, and Emily P. Balskus
mBio, 2015, Volume 6, Number 2
A. Ofer, J. Kreft, D. T. Logan, G. Cohen, I. Borovok, and Y. Aharonowitz
Journal of Bacteriology, 2011, Volume 193, Number 12, Page 2931
Brandon L. Greene, Alexander T. Taguchi, JoAnne Stubbe, and Daniel G. Nocera
Journal of the American Chemical Society, 2017
Lindsey R. F. Backman, Michael A. Funk, Christopher D. Dawson, and Catherine. L. Drennan
Critical Reviews in Biochemistry and Molecular Biology, 2017, Page 1
Joseph W. LaMattina, Nicholas D. Keul, Pierre Reitzer, Suraj Kapoor, Felipe Galzerani, Daniel J. Koch, Iuri E. Gouvea, and William N. Lanzilotta
Journal of Biological Chemistry, 2016, Volume 291, Number 30, Page 15515
Martin R. Challand, Enrico Salvadori, Rebecca C. Driesener, Christopher W. M. Kay, Peter L. Roach, James Spencer, and Katrin Karbstein
PLoS ONE, 2013, Volume 8, Number 7, Page e67979
B. J. Levin, Y. Y. Huang, S. C. Peck, Y. Wei, A. Martínez-del Campo, J. A. Marks, E. A. Franzosa, C. Huttenhower, and E. P. Balskus
Science, 2017, Volume 355, Number 6325, Page eaai8386
Joan B. Broderick and James D. Moody
Cell Chemical Biology, 2016, Volume 23, Number 10, Page 1173
Smaranda Bodea, Michael A. Funk, Emily P. Balskus, and Catherine L. Drennan
Cell Chemical Biology, 2016, Volume 23, Number 10, Page 1206
K. Zargar, R. Saville, R. M. Phelan, S. G. Tringe, C. J. Petzold, J. D. Keasling, and H. R. Beller
Scientific Reports, 2016, Volume 6, Number 1
Deniz Seyhan, Peter Friedrich, Maciej Szaleniec, Markus Hilberg, Wolfgang Buckel, Bernard T. Golding, and Johann Heider
Angewandte Chemie, 2016, Volume 128, Number 38, Page 11836
Deniz Seyhan, Peter Friedrich, Maciej Szaleniec, Markus Hilberg, Wolfgang Buckel, Bernard T. Golding, and Johann Heider
Angewandte Chemie International Edition, 2016, Volume 55, Number 38, Page 11664
Anjumala Herath, Boris Wawrik, Yujia Qin, Jizhong Zhou, Amy V. Callaghan, and Tillmann Lueders
FEMS Microbiology Ecology, 2016, Volume 92, Number 5, Page fiw062
Maciej Szaleniec and Johann Heider
International Journal of Molecular Sciences, 2016, Volume 17, Number 4, Page 514
Perry A. Frey, Adrian D. Hegeman, and Frank J. Ruzicka
Critical Reviews in Biochemistry and Molecular Biology, 2008, Volume 43, Number 1, Page 63
Xi Wei, Tetyana Gilevska, Felix Wetzig, Conrad Dorer, Hans-Hermann Richnow, and Carsten Vogt
Environmental Pollution, 2016, Volume 210, Page 166
Daniel Lundin, Gustav Berggren, Derek Logan, and Britt-Marie Sjöberg
Life, 2015, Volume 5, Number 1, Page 604
Nicholas D. Lanz and Squire J. Booker
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2015, Volume 1853, Number 6, Page 1316
Chandresh Thakker, Irene Martínez, Wei Li, Ka-Yiu San, and George N. Bennett
Journal of Industrial Microbiology & Biotechnology, 2015, Volume 42, Number 3, Page 403
Brinda Selvaraj, Antonio J. Pierik, Eckhard Bill, and Berta M. Martins
JBIC Journal of Biological Inorganic Chemistry, 2014, Volume 19, Number 8, Page 1317
Ilya A. Shkrob, Timothy W. Marin, James F. Wishart, and David C. Grills
The Journal of Physical Chemistry B, 2014, Volume 118, Number 35, Page 10477
M. A. Funk, E. T. Judd, E. N. G. Marsh, S. J. Elliott, and C. L. Drennan
Proceedings of the National Academy of Sciences, 2014, Volume 111, Number 28, Page 10161
Yasunori Tanji, Kazuya Toyama, Ryo Hasegawa, and Kazuhiko Miyanaga
Biochemical Engineering Journal, 2014, Volume 90, Page 114
Matthias Boll, Claudia Löffler, Brandon E. L. Morris, and Johannes W. Kung
Environmental Microbiology, 2014, Volume 16, Number 3, Page 612
Krista A. Shisler and Joan B. Broderick
Archives of Biochemistry and Biophysics, 2014, Volume 546, Page 64
Mikolaj Feliks, Berta M. Martins, and G. Matthias Ullmann
Journal of the American Chemical Society, 2013, Volume 135, Number 39, Page 14574
R. Hasegawa, K. Toyama, K. Miyanaga, and Y. Tanji
Applied Microbiology and Biotechnology, 2014, Volume 98, Number 4, Page 1853
Vivek S. Bharadwaj, Anthony M. Dean, and C. Mark Maupin
Journal of the American Chemical Society, 2013, Volume 135, Number 33, Page 12279
Brian R. Francis
Journal of Molecular Evolution, 2013, Volume 77, Number 4, Page 134
Brinda Selvaraj, Antonio J. Pierik, Eckhard Bill, and Berta M. Martins
JBIC Journal of Biological Inorganic Chemistry, 2013, Volume 18, Number 6, Page 633
Julien Jorda, David Lopez, Nicole M. Wheatley, and Todd O. Yeates
Protein Science, 2013, Volume 22, Number 2, Page 179
C. J. Thibodeaux and W. A. van der Donk
Proceedings of the National Academy of Sciences, 2012, Volume 109, Number 52, Page 21184
S. Craciun and E. P. Balskus
Proceedings of the National Academy of Sciences, 2012, Volume 109, Number 52, Page 21307
Michael Müller
Advanced Synthesis & Catalysis, 2012, Volume 354, Number 17, Page 3161
Nicholas D. Lanz and Squire J. Booker
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2012, Volume 1824, Number 11, Page 1196
Mikolaj Feliks and G. Matthias Ullmann
The Journal of Physical Chemistry B, 2012, Volume 116, Number 24, Page 7076
Markus Hilberg, Antonio J. Pierik, Eckhard Bill, Thorsten Friedrich, Marie-Luise Lippert, and Johann Heider
JBIC Journal of Biological Inorganic Chemistry, 2012, Volume 17, Number 1, Page 49
Heinz Wilkes, Andrea Vieth, and Rouven Elias
Organic Geochemistry, 2008, Volume 39, Number 8, Page 1215
Martin R. Challand, Rebecca C. Driesener, and Peter L. Roach
Natural Product Reports, 2011, Volume 28, Number 10, Page 1696
Berta M. Martins, Martin Blaser, Mikolaj Feliks, G. Matthias Ullmann, Wolfgang Buckel, and Thorsten Selmer
Journal of the American Chemical Society, 2011, Volume 133, Number 37, Page 14666
Rachel Narehood Austin and John T. Groves
Metallomics, 2011, Volume 3, Number 8, Page 775
Johann Heider
Current Opinion in Chemical Biology, 2007, Volume 11, Number 2, Page 188
Johnny Hioe, Gökcen Savasci, Harald Brand, and Hendrik Zipse
Chemistry - A European Journal, 2011, Volume 17, Number 13, Page 3781
Wolfgang Buckel and Bernard T. Golding
Annual Review of Microbiology, 2006, Volume 60, Number 1, Page 27
Chi-Kit Siu, Junfang Zhao, Julia Laskin, Ivan K. Chu, Alan C. Hopkinson, and K. W. Michael Siu
Journal of the American Society for Mass Spectrometry, 2009, Volume 20, Number 6, Page 996
E. Neil G. Marsh, Dustin P. Patterson, and Lei Li
ChemBioChem, 2010, Volume 11, Number 5, Page 604
Rudolf K. Thauer and Seigo Shima
Annals of the New York Academy of Sciences, 2008, Volume 1125, Number 1, Page 158
Johnny Hioe and Hendrik Zipse
Faraday Discuss., 2010, Volume 145, Page 301
Karmen Condic-Jurkic, Hendrik Zipse, and David M. Smith
Journal of Computational Chemistry, 2009, Page NA
Wolfgang Buckel
Angewandte Chemie, 2009, Volume 121, Number 37, Page 6911
Wolfgang Buckel
Angewandte Chemie International Edition, 2009, Volume 48, Number 37, Page 6779
J. L. Vey, J. Yang, M. Li, W. E. Broderick, J. B. Broderick, and C. L. Drennan
Proceedings of the National Academy of Sciences, 2008, Volume 105, Number 42, Page 16137
Olav Grundmann, Astrid Behrends, Ralf Rabus, Judith Amann, Thomas Halder, Johann Heider, and Friedrich Widdel
Environmental Microbiology, 2008, Volume 10, Number 2, Page 376
Christian Winderl, Sabine Schaefer, and Tillmann Lueders
Environmental Microbiology, 2007, Volume 9, Number 4, Page 1035

Comments (0)

Please log in or register to comment.
Log in