Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred


IMPACT FACTOR 2018: 3.014
5-year IMPACT FACTOR: 3.162

CiteScore 2018: 3.09

SCImago Journal Rank (SJR) 2018: 1.482
Source Normalized Impact per Paper (SNIP) 2018: 0.820

Online
ISSN
1437-4315
See all formats and pricing
More options …
Volume 386, Issue 3

Issues

Doxorubicin induces EGF receptor-dependent downregulation of gap junctional intercellular communication in rat liver epithelial cells

Kotb Abdelmohsen
  • Institut für Biochemie und Molekularbiologie I, Heinrich-Heine-Universität Düsseldorf, D-40225 Düsseldorf, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Claudia von Montfort
  • Institut für Biochemie und Molekularbiologie I, Heinrich-Heine-Universität Düsseldorf, D-40225 Düsseldorf, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Dominik Stuhlmann
  • Institut für Biochemie und Molekularbiologie I, Heinrich-Heine-Universität Düsseldorf, D-40225 Düsseldorf, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ P. Arne Gerber
  • Institut für Biochemie und Molekularbiologie I, Heinrich-Heine-Universität Düsseldorf, D-40225 Düsseldorf, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Ulrich K.M. Decking
  • Institut für Herz- und Kreislaufphysiologie, Heinrich-Heine-Universität Düsseldorf, D-40225 Düsseldorf, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Helmut Sies
  • Institut für Biochemie und Molekularbiologie I, Heinrich-Heine-Universität Düsseldorf, D-40225 Düsseldorf, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Lars-Oliver Klotz
  • Institut für Biochemie und Molekularbiologie I, Heinrich-Heine-Universität Düsseldorf, D-40225 Düsseldorf, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2005-07-05 | DOI: https://doi.org/10.1515/BC.2005.027

Abstract

Exposure of rat liver epithelial cells to doxorubicin, an anthraquinone derivative widely employed in cancer chemotherapy, led to a dose-dependent decrease in gap junctional intercellular communication (GJC). Gap junctions are clusters of inter-cellular channels consisting of connexins, the major connexin in the cells used being connexin-43 (Cx43). Doxorubicin-induced loss of GJC was mediated by activation of extracellular signal-regulated kinase (ERK)-1 and ERK-2, as demonstrated using inhibitors of ERK activation. Furthermore, activation of the epidermal growth factor (EGF) receptor by doxorubicin was responsible for ERK activation and the subsequent attenuation of GJC. Inhibition of GJC, however, was not by direct phosphorylation of Cx43 by ERK-1/2, whereas menadione, a 1,4-naphthoquinone derivative that was previously demonstrated to activate the same EGF receptor-dependent pathway as doxorubicin, resulting in downregulation of GJC, caused strong phos-phorylation of Cx43 at serines 279 and 282. Thus, ERK-dependent downregulation of GJC upon exposure to quinones may occur both by direct phosphorylation of Cx43 and in a phosphorylation-independent manner.

Keywords: connexin-43; extracellular signal-regulated kinase (ERK); gap junction; mitogen-activated protein (MAP) kinase; oxidative stress; signal transduction; stress signaling

About the article

Corresponding author


Received: October 28, 2004

Accepted: January 4, 2005

Published Online: 2005-07-05

Published in Print: 2005-03-01


Citation Information: Biological Chemistry, Volume 386, Issue 3, Pages 217–223, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2005.027.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Lars-Oliver Klotz, Xiaoqing Hou, and Claus Jacob
Molecules, 2014, Volume 19, Number 9, Page 14902
[2]
Lars-Oliver Klotz, Cristina Sánchez-Ramos, Ignacio Prieto-Arroyo, Pavel Urbánek, Holger Steinbrenner, and Maria Monsalve
Redox Biology, 2015, Volume 6, Page 51
[3]
James A. McCubrey, Linda S. Steelman, William H. Chappell, Stephen L. Abrams, Ellis W.T. Wong, Fumin Chang, Brian Lehmann, David M. Terrian, Michele Milella, Agostino Tafuri, Franca Stivala, Massimo Libra, Jorg Basecke, Camilla Evangelisti, Alberto M. Martelli, and Richard A. Franklin
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2007, Volume 1773, Number 8, Page 1263
[4]
Ingrit Hamann, Kerstin Petroll, Larson Grimm, Andrea Hartwig, and Lars-Oliver Klotz
Archives of Biochemistry and Biophysics, 2014, Volume 558, Page 42
[5]
Peter Gerber, Stephan Meller, Tatiana Eames, Bettina Buhren, Holger Schrumpf, Sonja Hetzer, Laura Ehmann, Wilfried Budach, Edwin Bölke, Christiane Matuschek, Andreas Wollenberg, and Bernhard Homey
European Journal of Medical Research, 2012, Volume 17, Number 1, Page 4
[6]
Niloofar Ale-Agha, Stefanie Galban, Christiane Sobieroy, Kotb Abdelmohsen, Myriam Gorospe, Helmut Sies, and Lars-Oliver Klotz
Hepatology, 2009, Volume 50, Number 5, Page 1567
[7]
Lars-Oliver Klotz
Archives of Biochemistry and Biophysics, 2012, Volume 524, Number 1, Page 23
[8]
Zhijuan Cao, Xuan Xu, Linli Que, Qi Chen, and Yuehua Li
Journal of Nanjing Medical University, 2009, Volume 23, Number 3, Page 163
[10]
Niloofar Ale-Agha, Catrin Albrecht, and Lars-Oliver Klotz
Biochemical and Biophysical Research Communications, 2009, Volume 390, Number 1, Page 44
[11]
Kotb Abdelmohsen, Elisabeth Sauerbier, Niloofar Ale-Agha, Juliane Beier, Philippe Walter, Stefanie Galban, Dominik Stuhlmann, Helmut Sies, and Lars-Oliver Klotz
Biochemical and Biophysical Research Communications, 2007, Volume 364, Number 2, Page 313
[12]
Brynjar Foss, Karl Johan Tronstad, and Øystein Bruserud
Biochimica et Biophysica Acta (BBA) - Biomembranes, 2010, Volume 1798, Number 1, Page 1
[13]
Claudia von Montfort, Niklas S. Fernau, Juliane I. Beier, Helmut Sies, and Lars-Oliver Klotz
Free Radical Biology and Medicine, 2006, Volume 41, Number 9, Page 1478
[14]
Brynjar Foss, Tor Hervig, and Øystein Bruserud
Stem Cells and Development, 2009, Volume 18, Number 6, Page 807
[15]
Niloofar Ale-Agha, Catrin Albrecht, and Lars-Oliver Klotz
Biological Chemistry, 2010, Volume 391, Number 11
[16]
Mukesh K. Nyati, Meredith A. Morgan, Felix Y. Feng, and Theodore S. Lawrence
Nature Reviews Cancer, 2006, Volume 6, Number 11, Page 876
[17]
Peng Yue, Yong Zhang, Zhimin Du, Jing Xiao, Zhenwei Pan, Ning Wang, Haiyan Yu, Wencai Ma, Hong Qin, Wen-Hui Wang, Dao-Hong Lin, and Baofeng Yang
Cellular Physiology and Biochemistry, 2006, Volume 17, Number 3-4, Page 129

Comments (0)

Please log in or register to comment.
Log in