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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred

IMPACT FACTOR 2018: 3.014
5-year IMPACT FACTOR: 3.162

CiteScore 2018: 3.09

SCImago Journal Rank (SJR) 2018: 1.482
Source Normalized Impact per Paper (SNIP) 2018: 0.820

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Volume 386, Issue 3


Quercetin metabolism in vital and apoptotic human leukaemia cells

Jutta Ludwig-Müller / Sergey V. Tokalov / Alexander Franz / Herwig O. Gutzeit
Published Online: 2005-07-05 | DOI: https://doi.org/10.1515/BC.2005.033


The metabolism of the flavonol quercetin in human leukaemia (HL-60) cells was investigated. The fluorescence that is elicited by quercetin upon binding to a target protein was quickly attenuated in vital cells, while apoptotic cells showed persistent fluorescence. The dynamics of induction and loss of fluorescence in the cells were quantified by flow cytometry. Several potential metabolites of quercetin, apart from isorhamnetin, had weak or no fluorogenic properties with test proteins. HPLC analysis showed that quercetin was metabolised to several substances, among them glycosylated metabolites. The loss of fluorescence in vital cells offers the unique opportunity to directly observe the metabolic conversion of quercetin in human cells.

Keywords: apoptosis; flavonol fluorescence; HL-60 cells; quercetin metabolism

About the article

Corresponding author

Received: October 29, 2004

Accepted: January 13, 2005

Published Online: 2005-07-05

Published in Print: 2005-03-01

Citation Information: Biological Chemistry, Volume 386, Issue 3, Pages 279–283, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2005.033.

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