Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred


IMPACT FACTOR 2018: 3.014
5-year IMPACT FACTOR: 3.162

CiteScore 2018: 3.09

SCImago Journal Rank (SJR) 2018: 1.482
Source Normalized Impact per Paper (SNIP) 2018: 0.820

Online
ISSN
1437-4315
See all formats and pricing
More options …
Volume 386, Issue 3

Issues

Quercetin metabolism in vital and apoptotic human leukaemia cells

Jutta Ludwig-Müller / Sergey V. Tokalov / Alexander Franz / Herwig O. Gutzeit
Published Online: 2005-07-05 | DOI: https://doi.org/10.1515/BC.2005.033

Abstract

The metabolism of the flavonol quercetin in human leukaemia (HL-60) cells was investigated. The fluorescence that is elicited by quercetin upon binding to a target protein was quickly attenuated in vital cells, while apoptotic cells showed persistent fluorescence. The dynamics of induction and loss of fluorescence in the cells were quantified by flow cytometry. Several potential metabolites of quercetin, apart from isorhamnetin, had weak or no fluorogenic properties with test proteins. HPLC analysis showed that quercetin was metabolised to several substances, among them glycosylated metabolites. The loss of fluorescence in vital cells offers the unique opportunity to directly observe the metabolic conversion of quercetin in human cells.

Keywords: apoptosis; flavonol fluorescence; HL-60 cells; quercetin metabolism

About the article

Corresponding author


Received: October 29, 2004

Accepted: January 13, 2005

Published Online: 2005-07-05

Published in Print: 2005-03-01


Citation Information: Biological Chemistry, Volume 386, Issue 3, Pages 279–283, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2005.033.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Dharambir Kashyap, Sonam Mittal, Katrin Sak, Paavan Singhal, and Hardeep Singh Tuli
Tumor Biology, 2016, Volume 37, Number 10, Page 12927
[2]
Fabio Nicolini, Olga Burmistrova, María Teresa Marrero, Fernando Torres, Cristina Hernández, José Quintana, and Francisco Estévez
Molecular Carcinogenesis, 2013, Page n/a
[3]
Marie Stiborova, Martina Rupertova, Heinz H. Schmeiser, and Eva Frei
Biomedical Papers, 2006, Volume 150, Number 1, Page 13
[4]
Kelly Carolina Frauzino Araújo, Eula Maria de M.B. Costa, Francine Pazini, Marize Campos Valadares, and Valéria de Oliveira
Food and Chemical Toxicology, 2013, Volume 51, Page 93
[5]
M. Böhl, C. Czupalla, S.V. Tokalov, B. Hoflack, and H.O. Gutzeit
Analytical Biochemistry, 2005, Volume 346, Number 2, Page 295
[6]
M. Stiborová, M. Rupertová, and E. Frei
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2011, Volume 1814, Number 1, Page 175
[7]
Sergey V. Tokalov, Andrij M. Abramyuk, and Nasreddin D. Abolmaali
Nutrition and Cancer, 2010, Volume 62, Number 6, Page 795
[8]
SUSANNE PÄSOLD, INA SIEGEL, CLAUDIA SEIDEL, and JUTTA LUDWIG-MÜLLER
Molecular Plant Pathology, 2010, Volume 11, Number 4, Page 545
[9]
Jitka Poljaková, Martin Dračínský, Eva Frei, Jiří Hudeček, and Marie Stiborová
Collection of Czechoslovak Chemical Communications, 2006, Volume 71, Number 8, Page 1169
[10]
Marie Stiborová, Jitka Poljaková, Helena Ryšlavá, Martin Dračínský, Tomáš Eckschlager, and Eva Frei
International Journal of Cancer, 2007, Volume 120, Number 2, Page 243

Comments (0)

Please log in or register to comment.
Log in