Jump to ContentJump to Main Navigation
Show Summary Details
In This Section

Thomas, Douglas D.

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Turk, Boris / Wittinghofer, Alfred

12 Issues per year


IMPACT FACTOR 2016: 3.273

CiteScore 2016: 3.01

SCImago Journal Rank (SJR) 2015: 1.607
Source Normalized Impact per Paper (SNIP) 2015: 0.751

Online
ISSN
1437-4315
See all formats and pricing
In This Section
Volume 386, Issue 5 (May 2005)

Issues

An extracellular carboxylesterase from the basidiomycete Pleurotus sapidus hydrolyses xanthophyll esters

Holger Zorn
  • Zentrum Angewandte Chemie, Institut für Lebensmittelchemie der Universität Hannover, Wunstorfer Straße 14, D-30453 Hannover, Germany
/ Henning Bouws
  • Zentrum Angewandte Chemie, Institut für Lebensmittelchemie der Universität Hannover, Wunstorfer Straße 14, D-30453 Hannover, Germany
/ Meike Takenberg
  • Zentrum Angewandte Chemie, Institut für Lebensmittelchemie der Universität Hannover, Wunstorfer Straße 14, D-30453 Hannover, Germany
/ Manfred Nimtz
  • Department of Structural Biology GBF, German Research Centre for Biotechnology, Mascheroder Weg 1, D-38124 Braunschweig, Germany
/ Rita Getzlaff
  • Department of Structural Biology GBF, German Research Centre for Biotechnology, Mascheroder Weg 1, D-38124 Braunschweig, Germany
/ Dietmar E. Breithaupt
  • Universität Hohenheim, Institut für Lebensmittelchemie, Garbenstraße 28, D-70599 Stuttgart, Germany
/ Ralf G. Berger
  • Zentrum Angewandte Chemie, Institut für Lebensmittelchemie der Universität Hannover, Wunstorfer Straße 14, D-30453 Hannover, Germany
Published Online: 2005-07-05 | DOI: https://doi.org/10.1515/BC.2005.052

Abstract

An extracellular enzyme capable of efficient hydrolysis of xanthophyll esters was purified from culture supernatants of the basidiomycete Pleurotus sapidus. Under native conditions, the enzyme exhibited a molecular mass of 430 kDa, and SDS-PAGE data suggested a composition of eight identical subunits. Biochemical characterisation of the purified protein showed an isoelectric point of 4.5, and ideal hydrolysis conditions were observed at pH 5.8 and 40°C. Partial amino acid sequences were derived from N-terminal Edman degradation and from mass spectrometric ab initio sequencing of internal peptides. An 1861-bp cDNA containing an open reading frame of 1641 bp was cloned from a cDNA library that showed ca. 40% homology to Candida rugosa lipases. The P. sapidus carboxylesterase represents the first enzyme of the lipase/esterase family from a basidiomycetous fungus that has been characterised at the molecular level.

Keywords: carotenoids; cDNA library; fungi; lipase

References

About the article

Corresponding author


Received: February 9, 2005

Accepted: March 22, 2005

Published Online: 2005-07-05

Published in Print: 2005-05-01



Citation Information: Biological Chemistry, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2005.052. Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
M. Rusch, M. Rühl, H. Zorn, and G. Hamscher
Lebensmittelchemie, 2014, Volume 68, Number 5, Page 96
[2]
Katerina Zelena, Nadine Eisele, and Ralf G. Berger
Biotechnology Advances, 2014, Volume 32, Number 8, Page 1382
[3]
Pramvadee Tepwong, Anupam Giri, and Toshiaki Ohshima
Mycoscience, 2012, Volume 53, Number 2, Page 102
[4]
Sven Krügener, Kateryna Zelena, Holger Zorn, Manfred Nimtz, and Ralf G. Berger
Journal of Molecular Catalysis B: Enzymatic, 2009, Volume 57, Number 1-4, Page 16
[5]
Amber Vanden Wymelenberg, Patrick Minges, Grzegorz Sabat, Diego Martinez, Andrea Aerts, Asaf Salamov, Igor Grigoriev, Harris Shapiro, Nik Putnam, Paula Belinky, Carlos Dosoretz, Jill Gaskell, Phil Kersten, and Dan Cullen
Fungal Genetics and Biology, 2006, Volume 43, Number 5, Page 343
[6]
D. Linke and R.G. Berger
Journal of Biotechnology, 2011, Volume 152, Number 4, Page 125
[7]
AHMET COLAK, YASEMIN CAMEDAN, ÖZLEM FAIZ, ERTUGRUL SESLI, and YAKUP KOLCUOĞLU
Journal of Food Biochemistry, 2009, Volume 33, Number 4, Page 482
[8]
Diana Linke, Manfred Nimtz, Ralf G. Berger, and Holger Zorn
Journal of the American Oil Chemists' Society, 2009, Volume 86, Number 5, Page 437
[9]
Kateryna Zelena, Sven Krügener, Stefan Lunkenbein, Holger Zorn, and Ralf G. Berger
Biotechnology Letters, 2009, Volume 31, Number 3, Page 395
[10]
Henning Bouws, Andreas Wattenberg, and Holger Zorn
Applied Microbiology and Biotechnology, 2008, Volume 80, Number 3, Page 381
[11]
Shimin Wu, Holger Zorn, Ulrich Krings, and Ralf G. Berger
Flavour and Fragrance Journal, 2007, Volume 22, Number 1, Page 53

Comments (0)

Please log in or register to comment.
Log in