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Licensed Unlicensed Requires Authentication Published by De Gruyter July 7, 2005

Characterisation of human histone H1x

  • Nicole Happel , Ekkehard Schulze and Detlef Doenecke
From the journal Biological Chemistry

Abstract

The members of the H1 histone family can be classified into three groups, which are the main class subtypes expressed in somatic cells, the developmental- and tissue-specific subtypes, and the replacement subtype H1°. Until now, the subtype H1x was not classified, since it has not yet been thoroughly examined. The results of this study show that H1x shares similarities but also exhibits slight differences in its biochemical behaviour in comparison to the main class H1 histones. In HeLa cells it is located in the nucleus and partially associated with nucleosomes. Nevertheless, it is, like H1°, mainly located in chromatin regions that are not affected by micrococcal nuclease digestion. Further common features of H1x and the replacement histone H1° are that the genes of both subtypes are solitarily located and give rise to polyadenylated mRNA. However, comparison of the inducibility of their expression revealed that their genes are regulated differentially.

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Corresponding author

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Published Online: 2005-07-07
Published in Print: 2005-06-01

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