Jump to ContentJump to Main Navigation
Show Summary Details
In This Section

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred

12 Issues per year


IMPACT FACTOR 2016: 3.273

CiteScore 2016: 3.01

SCImago Journal Rank (SJR) 2015: 1.607
Source Normalized Impact per Paper (SNIP) 2015: 0.751

Online
ISSN
1437-4315
See all formats and pricing
In This Section
Just Accepted

Issues

Incinerator fly ash provokes alteration of redox equilibrium and liberation of arachidonic acid in vitro

Susanne Fritsch
  • Forschungszentrum Karlsruhe, Institut für Toxikologie und Genetik, Abteilung für Molekulare Umwelttoxikologie, Hermann-von-Helmholtz-Platz 1, D-76344 Eggenstein-Leopoldshafen, Germany
/ Silvia Diabaté
  • Forschungszentrum Karlsruhe, Institut für Toxikologie und Genetik, Abteilung für Molekulare Umwelttoxikologie, Hermann-von-Helmholtz-Platz 1, D-76344 Eggenstein-Leopoldshafen, Germany
/ Harald F. Krug
  • Forschungszentrum Karlsruhe, Institut für Toxikologie und Genetik, Abteilung für Molekulare Umwelttoxikologie, Hermann-von-Helmholtz-Platz 1, D-76344 Eggenstein-Leopoldshafen, Germany
Published Online: 2006-11-02 | DOI: https://doi.org/10.1515/BC.2006.178

Abstract

Numerous epidemiological studies have associated exposure to ambient particulate matter (PM) with pulmonary and cardiovascular health effects. Macrophages as a part of the primary pulmonary defence system play a crucial role by generating pro- and anti-inflammatory mediators. The aim of the present study was to examine the effect of incinerator fly ash (MAF02) as a model of environmental particulate matter on the formation of reactive oxygen species (ROS) and their ability to induce oxidative stress in RAW264.7 macrophages. Furthermore, the liberation of arachidonic acid (AA) was observed. The interaction of MAF02 with macrophages caused increased mobilisation of AA, accompanied by enhanced expression of cyclooxygenase-2 (COX-2). The MAF02-induced AA liberation was found to depend on an increased intracellular calcium concentration. In addition, MAF02-induced liberation of AA was selectively blocked by an ERK1/2 pathway-specific inhibitor, while inhibition of the p38 MAPK activity had no effect. Fly ash was also observed to induce an increase in cellular glutathione (GSH) content and antioxidative enzyme haem oxygenase-1 (HO-1). In correlation, experiments with dichlorofluorescein demonstrated increased formation of ROS upon treatment with fly ash. In summary, incinerator fly ash induces oxidative stress to a certain extent, resulting in the onset of important mechanisms related to inflammation.

Keywords: cyclooxygenase-2; incinerator fly ash; macrophages; oxidative stress; particulate matter; reactive oxygen species

References

About the article

Corresponding author


Received: May 30, 2006

Accepted: August 2, 2006

Published Online: 2006-11-02

Published in Print: 2006-10-01



Citation Information: Biological Chemistry, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2006.178. Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Juciano Gasparotto, Nauana Somensi, Fernanda F. Caregnato, Thallita K. Rabelo, Kátia DaBoit, Marcos L.S. Oliveira, José C.F. Moreira, and Daniel P. Gelain
Science of The Total Environment, 2013, Volume 463-464, Page 743
[2]
Yutong Zhao, Peter V. Usatyuk, Irina A. Gorshkova, Donghong He, Ting Wang, Liliana Moreno-Vinasco, Alison S. Geyh, Patrick N. Breysse, Jonathan M. Samet, Ernst Wm. Spannhake, Joe G. N. Garcia, and Viswanathan Natarajan
American Journal of Respiratory Cell and Molecular Biology, 2009, Volume 40, Number 1, Page 19
[3]
Silvia Diabaté, Britta Bergfeldt, Diana Plaumann, Caroline Übel, and Carsten Weiss
Analytical and Bioanalytical Chemistry, 2011, Volume 401, Number 10, Page 3197
[4]
J.P. Stevens, J. Zahardis, M. MacPherson, B.T. Mossman, and G.A. Petrucci
Toxicology in Vitro, 2008, Volume 22, Number 7, Page 1768
[5]
Hanns-Rudolf Paur, Flemming R. Cassee, Justin Teeguarden, Heinz Fissan, Silvia Diabate, Michaela Aufderheide, Wolfgang G. Kreyling, Otto Hänninen, Gerhard Kasper, Michael Riediker, Barbara Rothen-Rutishauser, and Otmar Schmid
Journal of Aerosol Science, 2011, Volume 42, Number 10, Page 668
[6]
Susanne Fritsch-Decker, Tanja Both, Sonja Mülhopt, Hanns-Rudolf Paur, Carsten Weiss, and Silvia Diabaté
Particle and Fibre Toxicology, 2011, Volume 8, Number 1, Page 23
[7]
Yuh-Chin T. Huang, Edward D. Karoly, Lisa A. Dailey, Michael T. Schmitt, Robert Silbajoris, Donald W. Graff, and Robert B. Devlin
Journal of Toxicology and Environmental Health, Part A, 2011, Volume 74, Number 5, Page 296

Comments (0)

Please log in or register to comment.
Log in