Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred

IMPACT FACTOR 2018: 3.014
5-year IMPACT FACTOR: 3.162

CiteScore 2018: 3.09

SCImago Journal Rank (SJR) 2018: 1.482
Source Normalized Impact per Paper (SNIP) 2018: 0.820

See all formats and pricing
More options …
Just Accepted


DNA-binding properties of the recombinant high-mobility-group-like AT-hook-containing region from human BRG1 protein

Mahavir Singh / Loyola D'Silva / Tad A. Holak
Published Online: 2006-11-02 | DOI: https://doi.org/10.1515/BC.2006.184


The hBRG1 protein, a central ATPase of the human switching/sucrose non-fermenting (SWI/SNF) remodeling complex, has a catalytic ATPase domain, an AT-hook motif and a bromodomain. Bromodomains, found in many chromatin-associated proteins, recognize N-acetyl-lysine in histones and other proteins. The AT-hook motif, first described in the high-mobility group of non-histone chromosomal proteins HMGA1/2, is a DNA-binding motif. The AT-hook binds to the AT-rich DNA sequences in the minor groove of B-DNA in a non-sequence specific manner. AT-hook motifs have been identified in many other DNA-binding proteins. In this study we cloned and purified a fragment of hBRG1 encompassing the AT-hook region and the bromodomain. Nuclear magnetic resonance (NMR) and circular dichroism (CD) analyses show that the recombinant domains are structured. The functionality of subdomains was checked by assessing their interactions with N-acetylated peptides from histones and with DNA. Isothermal titration calorimetric (ITC) analysis demonstrates that the primary micromolar interaction is through the AT-hook motif. The AT-hook region binds to linear DNA by unwinding it. These properties resemble the characteristics of the HMGA1/2 proteins and their interaction with DNA.

Keywords: AT-hook; AT-hook-DNA interaction; BRG1; bromodomain; chromatin remodeling; histone acetylation; NMR; SWI/SNF

About the article

Corresponding author

Received: April 24, 2006

Accepted: July 7, 2006

Published Online: 2006-11-02

Published in Print: 2006-10-01

Citation Information: Biological Chemistry, Volume 387, Issue 10/11, Pages 1469–1478, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2006.184.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Taiji Ito, Hirotaka Watanabe, Nobutake Yamamichi, Shunsuke Kondo, Toshio Tando, Takeshi Haraguchi, Taketoshi Mizutani, Kouhei Sakurai, Shuji Fujita, Tomonori Izumi, Toshiaki Isobe, and Hideo Iba
Biochemical Journal, 2008, Volume 411, Number 1, Page 201
Kevin W. Trotter and Trevor K. Archer
Nuclear Receptor Signaling, 2008, Volume 6, Number 1, Page nrs.06004
R. L. Chandler, J. Brennan, J. C. Schisler, D. Serber, C. Patterson, and T. Magnuson
Molecular and Cellular Biology, 2013, Volume 33, Number 2, Page 265
Emma A. Morrison, Julio C. Sanchez, Jehnna L. Ronan, Daniel P. Farrell, Katayoun Varzavand, Jenna K. Johnson, Brian X. Gu, Gerald R. Crabtree, and Catherine A. Musselman
Nature Communications, 2017, Volume 8, Page 16080
Ghia Euskirchen, Raymond K. Auerbach, and Michael Snyder
Journal of Biological Chemistry, 2012, Volume 287, Number 37, Page 30897
Supratik Das, Baisakhi Banerjee, Maidul Hossain, Muruganandan Thangamuniyandi, Saumya Dasgupta, Nipa Chongdar, Gopinatha Suresh Kumar, Gautam Basu, and Sujit Basu
PLoS ONE, 2013, Volume 8, Number 7, Page e66581
Blaine Bartholomew
Annual Review of Biochemistry, 2014, Volume 83, Number 1, Page 671
Vo Cam Quy, Sergio Pantano, Giulia Rossetti, Mauro Giacca, and Paolo Carloni
Biology, 2012, Volume 1, Number 3, Page 277
Rong Zhong, Li Liu, Yao Tian, Ying Wang, Jing Tian, Bei-bei Zhu, Wei Chen, Jia-ming Qian, Li Zou, Min Xiao, Na Shen, Hong Yang, Jiao Lou, Qian Qiu, Jun-tao Ke, Xing-hua Lu, Zhen-ling Wang, Wei Song, Ti Zhang, Hui Li, Li Wang, and Xiao-ping Miao
Scientific Reports, 2015, Volume 4, Number 1
Yifeng Xu, Eng-Seng Gan, Yuehui He, and Toshiro Ito
Nucleus, 2013, Volume 4, Number 4, Page 274
Milana Frenkel-Morgenstern and Alfonso Valencia
Bioinformatics, 2012, Volume 28, Number 12, Page i67
Chun-wa Chung and David F. Tough
Drug Discovery Today: Therapeutic Strategies, 2012, Volume 9, Number 2-3, Page e111
Sagar Darvekar, Sylvia Sagen Johnsen, Agnete Bratsberg Eriksen, Terje Johansen, and Eva Sjøttem
Biochemical Journal, 2012, Volume 442, Number 1, Page 65
Beverly Duncan and Keji Zhao
DNA and Cell Biology, 2007, Volume 26, Number 10, Page 745
Oksana Okhrimenko and Ilian Jelesarov
Journal of Molecular Recognition, 2008, Volume 21, Number 1, Page 1

Comments (0)

Please log in or register to comment.
Log in