Jump to ContentJump to Main Navigation
Show Summary Details

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Turk, Boris / Wittinghofer, Alfred

SCImago Journal Rank (SJR) 2015: 1.607
Source Normalized Impact per Paper (SNIP) 2015: 0.751
Impact per Publication (IPP) 2015: 2.609

249,00 € / $374.00 / £187.00*

See all formats and pricing



Select Volume and Issue


30,00 € / $42.00 / £23.00

Get Access to Full Text

Suppression of TNF-α production by S-adenosylmethionine in human mononuclear leukocytes is not mediated by polyamines

Jingling Yu1 / Senja Sauter2 / Alexandr Parlesak3




Corresponding author

Citation Information: Biological Chemistry. Volume 387, Issue 12, Pages 1619–1627, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: 10.1515/BC.2006.202, November 2006

Publication History

March 21, 2006
August 22, 2006
Published Online:


Endotoxin-induced cytokine production is an important mechanism in the development of several types of liver damage. Methionine, some of its precursors and metabolites were reported to have protective effects against such injury. The aim of this study was to investigate whether methionine, its precursors or metabolites [phosphatidylcholine, choline, betaine, S-adenosylmethionine (SAM)] have a modulating effect on tumor necrosis factor α (TNF-α) production by endotoxin-stimulated human mononuclear leukocytes and whether SAM-dependent polyamines (spermidine, spermine) are mediators of SAM-induced inhibition of TNF-α synthesis. Methionine and betaine had a moderate stimulatory effect on TNF-α production, whereas phosphatidylcholine (ID50 5.4 mM), SAM (ID50 131 μM), spermidine (ID50 4.5 μM) and spermine (ID50 3.9 μM) had a predominantly inhibitory effect. Putrescine did not alter TNF-α release. Inhibitors of polyamine synthesis that blocked either putrescine (difluoromethylornithine) or spermine (CGP48664A) production did not affect TNF-α synthesis. Endotoxin stimulation of leukocytes did not alter the intracellular levels of polyamines. In addition, supplementation with SAM did not change the intracellular concentration of either polyamine measured. We conclude that phosphatidylcholine-induced immunosuppression is not caused by methionine and polyamines are not involved in SAM-induced inhibition of TNF-α production. The limitation of TNF-α release by spermidine is specific and is not due to its conversion into spermine.

Keywords: inflammation; lipopolysaccharide; methionine; putrescine; spermidine; spermine

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Carlos R. Cámara-Lemarroy, Francisco J. Guzmán-de la Garza, Paula Cordero-Pérez, Gabriela Alarcón-Galván, Liliana Torres-Gonzalez, Linda E. Muñoz-Espinosa, and Nancy E. Fernández-Garza
The Scientific World JOURNAL, 2011, Volume 11, Page 1886
E. Merlin, N. Goncalves-Mendes, D. Hannani, A. de la Torre, M.C. Farges, H. Laroye, F. Demeocq, J. Kanold, and M.P. Vasson
Transplant Immunology, 2011, Volume 24, Number 2, Page 100

Comments (0)

Please log in or register to comment.