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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred


IMPACT FACTOR 2018: 3.014
5-year IMPACT FACTOR: 3.162

CiteScore 2018: 3.09

SCImago Journal Rank (SJR) 2018: 1.482
Source Normalized Impact per Paper (SNIP) 2018: 0.820

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ISSN
1437-4315
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Volume 387, Issue 3

Issues

Inhibition of mRNA deadenylation and degradation by different types of cell stress

Gayatri Gowrishankar
  • Institute of Biochemistry, Medical School Hannover, Carl-Neuberg-Strasse 1, D-30625 Hannover, Germany and Present address: Department of Radiology, Stanford University School of Medicine, 1201 Welch Road, Stanford, CA 94305-5484, USA.
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Reinhard Winzen
  • Institute of Biochemistry, Medical School Hannover, Carl-Neuberg-Strasse 1, D-30625 Hannover, Germany
  • Other articles by this author:
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/ Oliver Dittrich-Breiholz
  • Institute of Pharmacology, Medical School Hannover, Carl-Neuberg-Strasse 1, D-30625 Hannover, Germany
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  • De Gruyter OnlineGoogle Scholar
/ Natalie Redich
  • Institute of Biochemistry, Medical School Hannover, Carl-Neuberg-Strasse 1, D-30625 Hannover, Germany
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/ Michael Kracht
  • Institute of Pharmacology, Medical School Hannover, Carl-Neuberg-Strasse 1, D-30625 Hannover, Germany
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  • De Gruyter OnlineGoogle Scholar
/ Helmut Holtmann
  • Institute of Biochemistry, Medical School Hannover, Carl-Neuberg-Strasse 1, D-30625 Hannover, Germany
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Published Online: 2006-03-17 | DOI: https://doi.org/10.1515/BC.2006.043

Abstract

We have previously observed rapid and strong inhibition of mRNA deadenylation and degradation in response to UV-B light [Gowrishankar et al., Biol. Chem. 386 (2005), pp. 1287–1293]. Expression analysis using a microarray for inflammatory genes showed that UV-B light induces stabilization of all short-lived mRNAs assayed. Stabilization was observed in HeLa cells, as well as in the keratinocyte line HaCaT. It affected constitutively expressed mRNA species, as well as species induced by the inflammatory cytokine IL-1. Many of the latter encode proteins involved in inflammation, suggesting that stress-induced inhibition of mRNA deadenylation contributes to changes in inflammatory gene expression. Deadenylation and degradation of tet-off-expressed mRNAs were also inhibited upon exposure to H2O2. However, scavengers of reactive oxygen species did not interfere with UV-B-induced inhibition of degradation, arguing against the involvement of UV-induced H2O2 in these effects of UV-B light. Heat shock and hyperosmolarity also inhibited mRNA deadenylation and degradation, whereas γ-radiation did not. Thus, inhibition of mRNA deadenylation and degradation is a cellular response elicited by several but not all inducers of cell stress.

Keywords: cytokines; inflammation; mRNA stability; poly(A)-tail; stress; UV light

About the article

Corresponding author


Received: November 15, 2005

Accepted: December 19, 2005

Published Online: 2006-03-17

Published in Print: 2006-03-01


Citation Information: Biological Chemistry, Volume 387, Issue 3, Pages 323–327, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2006.043.

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